What are CPGs?
Controlled Pore Glasses (CPGs) are made of silica and are widely used in solid phase synthesis reactions of oligonucleotides. CPG spheres have many irregular pore channels and are pore size stable. For spatial reasons, large pore sizes are suitable for the synthesis of long fragments, and short pore sizes are suitable for the synthesis of short fragments. BOC Sciences offers a wide range of CPGs for oligonucleotide synthesis to choose from, with pore sizes ranging from 300 Å-5000 Å. Groups linked to CPGs include TAMRA, DMT-Phosphate, Fluorescein, Aminopropyl, etc.
Different pore size CPGs can be used to synthesize oligonucleotide products with different loadings:
(1) 500 Å/600 Å pore size CPG products are suitable for oligonucleotide synthesis of < 35 mers. For example, therapeutic oligonucleotides can be synthesized at loads up to 100 μmol/g.
(2) 1000 Å pore size CPG products for >35 mers or highly modified oligonucleotide synthesis.
(3) 2000 Å/3000 Å pore size CPG products, suitable for oligonucleotide synthesis on a scale of 80 mers or more, with synthetic loads typically ranging from 10 to 20 μmol/g.
Examples of CPGs
Because tetramethylrhodamine (TAMRA) is not base-stable, procedures for cleaving and deprotecting labeled oligonucleotides must be carefully considered. For example, to simplify the preparation of TAMRA oligonucleotides, 3'-TAMRA CPG for 3' labeling and TAMRA-dT for in-sequence labeling are available.
Fluorescein CPG is traditionally used to add fluorescein labeling to the 3'-end. For example, 3'-(6-FAM) CPG effectively blocks polymerase extension and exonuclease digestion at the 3'-end.
The phosphate functional group on the surface of DMT-Phosphate-CPG usually carries a DMT protecting group. DMT is a commonly used protecting group that protects the phosphate group from non-specific reactions during nucleotide synthesis. The DMT protecting group can be easily removed at the end of the synthesis to expose the phosphate group for further modification. CPGs have a pore size and pore structure that can be tuned similar to that of conventional CPGs. This controllable pore size facilitates efficient solid-phase synthesis of oligonucleotides and ensures good attachment and stability of the phosphate functional groups.
Aminopropyl-CPG is a CPG modified by an aminopropyl functional group, and we can provide products with pore sizes ranging from 300 Å-2000 Å. The products can be used for the amination modification of nucleotides, and by introducing an amino functional group, the chemistry and reactivity of nucleotides can be extended.
Features of CPGs
- Adjustable Pore Size and Pore Structure
The pore size and pore structure of CPGs can be tuned as needed, typically in the range of 3-30 nanometers. This controllable pore size and pore structure helps ensure efficient solid phase synthesis of oligonucleotides.
CPGs have a large amount of surface activity, providing enough binding sites to allow nucleotide immobilization and chemical reactions to take place during the synthesis process.
CPGs have good chemical stability and can be used under common nucleotide synthesis reaction conditions. They can tolerate synthesis conditions such as alkaline conditions, organic solvents, and high temperatures, thus ensuring high-quality oligonucleotide synthesis.
CPGs can be scaled and adjusted as needed for both small-scale research and large-scale industrial production.
After years of development, BOC Sciences has the expertise and experience to help you synthesize high-quality CPGs for oligo synthesis and accelerate the achievement of your research goals.
If you have any special requirements for CPGs products, please feel free to contact us. We look forward to working with you on attractive projects.
* Only for research. Not suitable for any diagnostic or therapeutic use.
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