Circular oligonucleotides are essentially covalently closed strands of DNA or RNA which are being heavily investigated as a new class of potential therapeutic agents. As a professional oligonucleotide synthesis company, BOC RNA provides circular oligonucleotide synthesis services to meet the diverse needs of our customers.
Circular oligonucleotides (CON) are covalently closed strands of DNA or RNA in essence. They usually have nucleotide loops at both ends with high melting transition temperature values. This strong secondary structure makes such circles easier to construct than those lacking a strong internal structure, since linear circular precursors fold themselves into a dumbbell shape with a cut in solution. Compared with the linear structures, these circular oligonucleotides have higher affinity, sequence selectivity, and are more resistant to nucleases, endowing them resistant to degradation by many cellular DNA and RNA decay mechanisms.
Fig. 1 Circular oligonucleotides (trimer through hexamer). (Smietana M, 2002)
Circular DNA or RNA oligonucleotides can be chemically synthesized from partially protected linear precursors using solution and solid-phase synthesis methods. After removal of linear DNA, circular oligonucleotides were purified by denaturing polyacrylamide gel electrophoresis (PAGE). Circularity was determined by exonuclease cleavage. Finally, an independent QA procedure was used to ensure the highest quality of each cyclic oligonucleotide.
Fig. 2 Synthesis of circular oligodinucleotides. (Smietana M, 2002)
For additional information regarding custom circular oligonucleotides synthesis, please feel free to contact us.
| Type |
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| Length | 35 to 200 mers |
| Quantities |
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| Purification | HPLC or PAGE |
| Validation | MS and PAGE analysis plus additional QA procedures |
| Price | Inquiry |
Circular oligonucleotides are covalently closed strands of DNA or RNA that have nucleotide loops at both ends. These unique structures offer higher affinity, sequence selectivity, and increased resistance to nuclease degradation compared to linear oligonucleotides.
Circular DNA or RNA oligonucleotides are synthesized from partially protected linear precursors using either solution or solid-phase synthesis methods. The linear precursors are cyclized, and the circular structures are then purified using denaturing PAGE and validated through exonuclease cleavage.
Circular oligonucleotides have various applications, including molecular cloning, phage therapy, nanotechnology, miRNA delivery, diagnostics, DNA polymerase inhibition, and rolling circle amplification (RCA), among others.
BOC Sciences offers custom synthesis of both circular DNA and RNA oligonucleotides, with lengths ranging from 35 to 200 mers. These oligonucleotides can be synthesized for a wide range of applications and can be tailored to meet specific research needs.
Yes, BOC Sciences works closely with customers to customize the synthesis of circular oligonucleotides, including factors like nucleotide sequence, loop size, and pairing methods, to meet the specific requirements of each project.
The process involves confirming the customer's requirements, designing the synthesis protocol, synthesizing nucleotide monomers, performing cyclization reactions to form the circular structure, and purifying and analyzing the final product to ensure high quality.
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