Circular oligonucleotides are essentially covalently closed strands of DNA or RNA which are being heavily investigated as a new class of potential therapeutic agents. As a professional oligonucleotide synthesis company, BOC RNA provides circular oligonucleotide synthesis services to meet the diverse needs of our customers.
Overview of Circular oligos
Circular oligonucleotides (CON) are covalently closed strands of DNA or RNA in essence. They usually have nucleotide loops at both ends with high melting transition temperature values. This strong secondary structure makes such circles easier to construct than those lacking a strong internal structure, since linear circular precursors fold themselves into a dumbbell shape with a cut in solution. Compared with the linear structures, these circular oligonucleotides have higher affinity, sequence selectivity, and are more resistant to nucleases, endowing them resistant to degradation by many cellular DNA and RNA decay mechanisms.
Fig. 1 Circular oligonucleotides (trimer through hexamer). (Smietana M, 2002)
Circular DNA or RNA oligonucleotides can be chemically synthesized from partially protected linear precursors using solution and solid-phase synthesis methods. After removal of linear DNA, circular oligonucleotides were purified by denaturing polyacrylamide gel electrophoresis (PAGE). Circularity was determined by exonuclease cleavage. Finally, an independent QA procedure was used to ensure the highest quality of each cyclic oligonucleotide.
Fig. 2 Synthesis of circular oligodinucleotides. (Smietana M, 2002)
Content of Custom CON Synthesis
For additional information regarding custom circular oligonucleotides synthesis, please feel free to contact us.
Type |
|
Length | 35 to 200 mers |
Quantities |
|
Purification | HPLC or PAGE |
Validation | MS and PAGE analysis plus additional QA procedures |
Price | Inquiry |
References