The advancement of RNA-based technologies has necessitated sophisticated chemical modifications to enhance RNA stability, functionality, and biological activity. Among these, the incorporation of acp3U (3-(3-Amino-3-carboxypropyl)uridine) into RNA molecules represents a cutting-edge modification designed to improve RNA performance in various research and development settings. BOC Sciences offers a highly specialized custom Acp3U modified RNA synthesis service, tailored to meet the stringent demands of scientific research, molecular biology, and therapeutic development. Our expertise ensures that researchers receive high-purity, precisely modified RNA constructs optimized for their experimental needs.
The Acp3U modification introduces a 3-aminopropyl group at the 3-position of uridine, profoundly influencing RNA's biochemical and biophysical properties:
Recognizing the unique demands of each research project, BOC Sciences delivers highly customizable Acp3U modified RNA synthesis services. Clients can specify RNA length, sequence composition, and exact positions for Acp3U incorporation, enabling precise molecular engineering. Purity requirements are adjustable, with options ranging from standard research-grade to ultra-pure preparations suitable for sensitive assays. Additionally, modifications can be combined with other chemical groups, including terminal labels or backbone alterations, to fulfill complex experimental needs. Delivery formats are flexible, offering lyophilized powder or solution-phase RNA, tailored to storage and application preferences. Throughout the customization process, expert consultation ensures optimal design and synthesis strategies that align with project objectives and timelines.
BOC Sciences specializes in providing a comprehensive range of Acp3U modifications designed to meet the specific functional and structural demands of advanced RNA research. The primary form is the canonical 3-(3-Amino-3-carboxypropyl)uridine (acp3U), which introduces a dual-functional group featuring both amino and carboxypropyl moieties at the 3-position of uridine. This modification enhances hydrogen bonding potential and overall RNA conformational stability, improving resistance to enzymatic degradation. Beyond this standard form, we offer specialized Acp3U derivatives that include additional functional groups for targeted applications:
Each Acp3U modification is synthesized with stringent control to maintain RNA integrity and precise positioning within the sequence, ensuring reproducible biochemical properties and compatibility with downstream applications. This diversity in modification types empowers researchers to tailor RNA molecules precisely to their experimental or preclinical development requirements.
BOC Sciences supports the incorporation of Acp3U modifications across a wide spectrum of RNA molecule types, addressing diverse research and development needs with precision and flexibility. Our capabilities encompass:
By accommodating these RNA types with custom Acp3U modifications, BOC Sciences ensures that researchers have access to optimized RNA molecules tailored to their experimental and developmental requirements.
Achieving precise control over the placement and number of Acp3U modifications is critical for tailoring RNA molecules to specific research objectives. At BOC Sciences, we offer both site-specific and multiple Acp3U incorporation options, each serving distinct experimental purposes:
| Site-specific or multiple Acp3U Modification | Description | Price |
| Site-Specific Incorporation | This approach involves the targeted insertion of a single Acp3U modification at a predefined nucleotide position within the RNA sequence. Such precision allows researchers to probe the structural and functional consequences of modification at specific loci, enabling detailed mechanistic studies. Site-specific Acp3U incorporation is particularly valuable when investigating RNA-protein interactions, fine-tuning RNA stability, or modulating local folding dynamics without altering the overall molecular framework. Our synthesis platform ensures high coupling efficiency and minimal by-products, preserving the integrity of the RNA strand with exact placement of the modification. | Inquiry |
| Site-Specific Incorporation | Incorporating multiple Acp3U residues throughout an RNA strand amplifies the functional benefits conferred by the modification. This strategy enhances overall RNA stability by increasing resistance to enzymatic degradation and improving structural robustness. Additionally, multiple Acp3U sites can serve as conjugation anchors for attaching diverse functional groups—such as fluorophores, affinity tags, or crosslinkers—enabling multifunctional RNA constructs for advanced biochemical assays or therapeutic development. Our optimized synthetic protocols accommodate complex modification patterns, maintaining high fidelity even in longer or heavily modified RNA molecules. | Inquiry |
By providing flexible incorporation strategies, BOC Sciences empowers clients to customize RNA constructs that precisely match their experimental design and performance requirements. Whether requiring pinpoint accuracy with single-site modifications or broad enhancement via multiple insertions, our Acp3U synthesis services deliver exceptional quality and reliability.
At BOC Sciences, the synthesis of Acp3U modified RNA follows a rigorously controlled, technically refined workflow designed to ensure precision, reproducibility, and chemical integrity. Each stage is handled by experienced chemists using advanced instrumentation and quality standards tailored for high-fidelity RNA modification.
Clients provide RNA sequences and define desired Acp3U incorporation sites. Our team conducts a comprehensive assessment, offering strategic input on modification feasibility, sequence optimization, and potential synthesis constraints.
Acp3U phosphoramidites—along with any additional chemical moieties—are synthesized or sourced, verified for purity, and prepared for incorporation under optimized reaction conditions.
Using state-of-the-art synthesizers, RNA strands are assembled base by base, with Acp3U residues introduced at designated positions through precision coupling protocols that preserve base integrity and structural fidelity.
The RNA is released from the solid support and chemically deprotected using conditions that maintain Acp3U's functional groups while minimizing structural degradation or side reactions.
Multiple chromatographic techniques—such as reverse-phase and ion-exchange HPLC—are employed to isolate the target product, removing truncated sequences, side products, and unreacted reagents.
Each batch undergoes rigorous analysis by MALDI-TOF or ESI-MS and analytical HPLC to verify molecular weight, chemical integrity, and sequence fidelity. Documentation is generated to support full traceability.
Final products are aliquoted under RNase-free conditions, lyophilized or formulated per customer request, and shipped with detailed Certificates of Analysis (CoA) and handling instructions.
This systematic workflow ensures BOC Sciences delivers Acp3U modified RNA with exceptional purity, reproducibility, and experimental reliability.
Decades of experience in RNA chemical synthesis ensure the highest quality and reliability in producing complex modified oligonucleotides.
Integration of advanced synthesis platforms and purification systems guarantees superior batch-to-batch consistency and product integrity.
Our team can accommodate diverse RNA lengths, modification patterns, and scale requirements to meet unique research demands.
Clients receive professional guidance from RNA synthesis experts throughout project execution, facilitating smooth workflows and successful outcomes.
Strict adherence to industry standards and confidentiality agreements protects intellectual property and research integrity.
Acp3U-modified RNA is not a general-purpose modification—it serves highly specific, strategic roles in advancing RNA-based research. At BOC Sciences, our clients utilize Acp3U modified RNA in precise applications where the introduction of the 3-(3-amino-3-carboxypropyl)uridine confers structural, functional, or chemical advantages.
Acp3U's unique side chain provides a reactive handle and enhanced hydrogen bonding capabilities, making it ideal for probing secondary and tertiary RNA structures. Researchers incorporate acp3U into functionally significant RNA regions to stabilize specific conformations or to investigate folding dynamics. Furthermore, acp3U serves as a site-specific label or crosslinking anchor in footprinting assays and CLIP-seq workflows for identifying protein-binding sites at nucleotide resolution.
The amino and carboxyl groups of acp3U offer orthogonal reactivity for chemical conjugation. Scientists exploit this for tethering biotin, fluorophores, photo-reactive groups, or affinity tags directly to RNA without disrupting base pairing or enzymatic recognition. Such functionalization is critical for pull-down assays, single-molecule imaging, biosensor development, and RNA tracking in live-cell systems.
Acp3U is a naturally occurring post-transcriptional modification found in the anticodon loop of certain tRNAs. Synthetic incorporation of acp3U enables mechanistic studies into translational fidelity, codon-anticodon recognition, and the biological impact of hypo- or hyper-modified tRNAs. By mimicking endogenous modifications in synthetic systems, researchers can dissect the functional relevance of acp3U in decoding accuracy and ribosomal interaction.
While not as extensively stabilizing as certain sugar modifications, acp3U can subtly influence nuclease resistance and improve RNA persistence in cellular or enzymatic assays. This is especially useful when acp3U is deployed in conjunction with other stabilizing modifications in structured RNA motifs or aptamers used in synthetic biology platforms.
Acp3U's side chain can be chemically activated or modified to serve as a site-specific crosslinking site. This has enabled advanced studies in structural proteomics, where RNA-protein complexes are stabilized via UV or chemical crosslinking at defined positions, allowing detailed structural elucidation by mass spectrometry or NMR.
In emerging synthetic biology and nanotechnology applications, acp3U-modified sites serve as anchoring points for attaching catalytic moieties, small-molecule ligands, or structural elements. This modular functionality allows the design of programmable RNA-based devices for molecular computation, gene regulation, or smart drug delivery systems.
BOC Sciences supports these advanced applications by offering highly controlled, position-specific Acp3U incorporation into a wide range of RNA formats, ensuring compatibility with demanding experimental systems in structural biology, synthetic biology, and molecular diagnostics.
Acp3U modifications enhance RNA thermal and enzymatic stability, reduce innate immune recognition, and enable versatile chemical conjugation. These improvements make the RNA more durable, bioactive, and functionally adaptable in downstream assays.
We support multiple site-specific Acp3U incorporations, depending on sequence length and design feasibility. Our chemists will evaluate structural compatibility to ensure synthesis success.
Absolutely. Our scientific team offers free consultation to evaluate sequence context, functional goals, and experimental applications to recommend optimal modification sites.
Yes. Acp3U contains a functional side chain that can serve as a chemically reactive site for fluorophore, biotin, or drug conjugation using standard coupling chemistries.
While Acp3U-modified RNA is typically synthesized chemically, its structural integrity does not interfere with most downstream enzymatic manipulations. For critical enzymatic steps, we recommend validating compatibility experimentally or consulting our experts.
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