BOC Sciences' RNAi transfection optimization services include siRNA delivery optimization and plasmid transfection optimization. As both siRNA and RNA interference vectors need to be transferred into cells in order to function. The efficient transfer of RNA interference products into cells is therefore a critical step for successful RNA interference experiments. Professional siRNA and plasmid transfection techniques can be used to optimize transfection efficiency and enhance the effectiveness of RNA interference.
The phenomenon of RNA interference (RNAi), which is frequently present in living organisms, is a sequence-specific post-transcriptional silencing process of genes that is sparked by double-stranded RNA homologous to the sequence of the target gene. The cytoplasmic enzyme Dicer breaks down dsRNA into siRNAs, which include 21-25 nucleotides. The RNA-induced silencing complex (RISC), which specifically binds to the homologous area of exogenous gene expression mRNAs and cleaves the mRNAs at the binding site, is created in vivo when the siRNAs attach to proteins. The degraded, damaged mRNAs are then used to prevent the associated gene's post-transcriptional mechanism for silencing from working.
RNAi has emerged as a crucial tool for understanding gene function and will be crucial in the treatment of viral diseases, genetic disorders, and oncological diseases by specifically inhibiting the overexpression of genes like HIV, hepatitis, oncogenes, and other related genes and keeping them in a silent or dormant state.
The application of RNAi technology is limited by transfection. The most common and crucial transfection for RNAi technology is siRNA transfection. siRNA transfection experiments involve the following important parameters:
|293 cell interference efficiency verification service||The constructed RNA interference plasmids are transfected with 293 cells and the interference efficiency is verified by fluorescence quantitative RT-PCR or Western-blot.|
|Customer cell line transfection optimization and interference efficiency verification service||Transfection efficiency is a key step for the success of RNA interference. Experiments are performed to improve RNA interference efficiency by optimizing transfection efficiency and validating RNA interference efficiency in customer-specified cells.|
|siRNA interference efficiency verification service||Pre-transfection experiments are performed using siRNA transfection reagents to optimize transfection efficiency, and interference efficiency is verified by fluorescence quantitative RT-PCR or Western blot.|