Psoralen Labeling of Oligonucleotides

Psoralen labeling of oligonucleotides is a common experimental technique used to introduce psoralen derivatives into oligonucleotide molecules. BOC Sciences offers a wide range of psoralen derivative labeling oligonucleotides and is constantly expanding its development in this area.

Fig 1. Unique crosslinking properties of Psoralen-conjugated oligonucleotides. (Nakao et al., 2023)

Advantages of Psoralen Labeling

• Photosensitivity

Psoralen is a photosensitive compound that can be activated by UV light irradiation to activate its cross-linking and labeling capabilities. This photosensitivity makes psoralen labeling a controlled and localizable labeling method. By precisely controlling the light conditions, DNA or RNA can be labeled or crosslinked within a specific time and space frame.

• High specificity

Because psoralens form covalent crosslinks with purine bases in nucleic acids (usually thymine), labeling occurs on nucleic acid molecules with a specific sequence or structure. This specific labeling makes psoralens very useful in studying DNA structure, protein-DNA interactions, etc.

• Reversibility

An important advantage of psoralen labeling is that crosslinks can be reversed under the right conditions. By using the property of guanine acids in DNA or RNA to cross-link with psoralen, the cross-links can be undone by short-wave UV irradiation or other methods, allowing the effects of labeling or cross-linking to be undone.

BOC Sciences' Psoralen-Labeled Oligonucleotide Services

BOC Sciences has the experience and technology to label oligonucleotides with many types of molecules, providing oligonucleotides with a variety of advantages in application and development. We currently provide our customers with 8-Aminopsoralen, 4'-Aminomethyltrioxsalen and other psoralen derivatives labeling oligonucleotide services, and provide subsequent validation and application development for the customized products.

• 8-Aminopsoralen labeling oligonucleotide service

BOC Sciences provides 8-Aminopsoralen labeling oligonucleotide services, which enable oligonucleotides to be used in a variety of applications. Our researchers prepare labeled oligonucleotides by customizing the oligonucleotide, selecting the oligonucleotide of the appropriate length and sequence, and then combining the two to obtain the target marker molecule.

• 4'-Aminomethyltrioxsalen labeling oligonucleotide service

4'-Aminomethyltrioxsalen (AMT) is a commonly used trioxylated psoralen derivative. BOC Sciences provides AMT labeling oligonucleotide services, including protocol design, development, and optimization of the reaction process, as well as analysis and validation of the product after completion of these procedures to ensure the reliability of the experimental results.

In addition to the above services, we can also customize the labeling of other types of Psoralen derivatives according to the requirements of our customers, which will bring more convenience to the development of your project.

Steps for Psoralen Labeling Oligonucleotide

• Preparation of Psoralen

We can provide many types of Psoralens, including 8-Aminopsoralen, 4'-Aminomethyltrioxsalen, and others. The selection of Psoralen can be based on customer's requirement and application.

• Preparation of oligonucleotides

BOC Sciences has products related to oligonucleotides, and if what the customer needs is not in our product list, we can customize it for you.

• Labeling of oligonucleotides

First, in a test tube containing an alkaline buffer, the Psoralen and oligonucleotide are mixed. Then, the mixture in the test tube is exposed to a UV lamp at room temperature to activate the photocrosslinking ability of the Psoralen. Finally, an appropriate amount of stopper is added to neutralize the reaction, resulting in a Psoralen-labeled oligonucleotide.

BOC Sciences has the skills and capabilities in oligonucleotide modification to efficiently fulfill customer requirements within the delivery time. If you are interested in our services, feel free to contact us.

Reference

1. Nakao J, et al. Unique Crosslinking Properties of Psoralen‐Conjugated Oligonucleotides Developed by Novel Psoralen N‐Hydroxysuccinimide Esters[J]. ChemBioChem, 2023: e202200789.

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Psoralen Labeling of Oligonucleotides

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