Psoralen labeling of oligonucleotides is a common experimental technique used to introduce psoralen derivatives into oligonucleotide molecules. BOC Sciences offers a wide range of psoralen derivative labeling oligonucleotides and is constantly expanding its development in this area.
Fig 1. Unique crosslinking properties of Psoralen-conjugated oligonucleotides. (Nakao et al., 2023)
Psoralen is a photosensitive compound that can be activated by UV light irradiation to activate its cross-linking and labeling capabilities. This photosensitivity makes psoralen labeling a controlled and localizable labeling method. By precisely controlling the light conditions, DNA or RNA can be labeled or crosslinked within a specific time and space frame.
Because psoralens form covalent crosslinks with purine bases in nucleic acids (usually thymine), labeling occurs on nucleic acid molecules with a specific sequence or structure. This specific labeling makes psoralens very useful in studying DNA structure, protein-DNA interactions, etc.
An important advantage of psoralen labeling is that crosslinks can be reversed under the right conditions. By using the property of guanine acids in DNA or RNA to cross-link with psoralen, the cross-links can be undone by short-wave UV irradiation or other methods, allowing the effects of labeling or cross-linking to be undone.
BOC Sciences has the experience and technology to label oligonucleotides with many types of molecules, providing oligonucleotides with a variety of advantages in application and development. We currently provide our customers with 8-Aminopsoralen, 4'-Aminomethyltrioxsalen and other psoralen derivatives labeling oligonucleotide services, and provide subsequent validation and application development for the customized products.
BOC Sciences provides 8-Aminopsoralen labeling oligonucleotide services, which enable oligonucleotides to be used in a variety of applications. Our researchers prepare labeled oligonucleotides by customizing the oligonucleotide, selecting the oligonucleotide of the appropriate length and sequence, and then combining the two to obtain the target marker molecule.
4'-Aminomethyltrioxsalen (AMT) is a commonly used trioxylated psoralen derivative. BOC Sciences provides AMT labeling oligonucleotide services, including protocol design, development, and optimization of the reaction process, as well as analysis and validation of the product after completion of these procedures to ensure the reliability of the experimental results.
In addition to the above services, we can also customize the labeling of other types of Psoralen derivatives according to the requirements of our customers, which will bring more convenience to the development of your project.
We can provide many types of Psoralens, including 8-Aminopsoralen, 4'-Aminomethyltrioxsalen, and others. The selection of Psoralen can be based on customer's requirement and application.
BOC Sciences has products related to oligonucleotides, and if what the customer needs is not in our product list, we can customize it for you.
First, in a test tube containing an alkaline buffer, the Psoralen and oligonucleotide are mixed. Then, the mixture in the test tube is exposed to a UV lamp at room temperature to activate the photocrosslinking ability of the Psoralen. Finally, an appropriate amount of stopper is added to neutralize the reaction, resulting in a Psoralen-labeled oligonucleotide.
BOC Sciences has the skills and capabilities in oligonucleotide modification to efficiently fulfill customer requirements within the delivery time. If you are interested in our services, feel free to contact us.
Psoralen labeling involves attaching psoralen derivatives to oligonucleotides, allowing for controlled cross-linking and specific sequence targeting. This technique enhances studies on DNA structure and protein-DNA interactions.
Psoralen offers photosensitivity, enabling controlled cross-linking upon UV light exposure. It also provides high specificity for purine bases and can reversibly cross-link nucleic acids under certain conditions.
BOC Sciences offers several psoralen derivatives, including 8-Aminopsoralen and 4'-Aminomethyltrioxsalen (AMT). These derivatives can be customized for different applications based on customer needs.
Psoralen and oligonucleotides are mixed in an alkaline buffer, then exposed to UV light, activating the psoralen's cross-linking ability. The reaction is neutralized, yielding the labeled oligonucleotides.
Psoralen's photosensitivity allows researchers to control the timing and location of cross-linking through UV exposure, offering greater precision and flexibility in experimental conditions and data interpretation.
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