At BOC Sciences, we specialize in providing high-quality mRNA capping services to accelerate your research and development processes. Our cutting-edge 5' capping technology ensures enhanced mRNA stability, improved translation efficiency, and seamless nuclear export, all of which are essential for successful gene expression, mRNA vaccine development, and gene therapy applications. With our expertise in RNA modifications, we help researchers achieve more reliable, consistent, and efficient results, supporting breakthroughs in biotechnology and pharmaceutical research. Trust us to optimize your mRNA-based projects and advance your scientific goals.
Messenger RNA (mRNA) is the template that guides the synthesis of proteins and is the messenger that transmits genetic information from DNA to proteins. The structure of mature mRNA has five main parts, from 5' to 3' including 5' cap, 5' UTR, open reading frame (ORF), 3' UTR and a PolyA tail.
| Structure of mature mRNA | Description |
| 5' cap | m7GPPPN structure, also called methylguanosine cap. Formed by RNA triphosphatase, mRNA guanyltransferase, mRNA (guanine-7) methyltransferase and mRNA (nucleoside-2'-)-methyltransferase. |
| 5' UTR | Refers to the region of mature mRNA located upstream of the coding region (CDS) that is not translated into protein. |
| ORF | The normal nucleotide sequence of a structural gene, the reading frame from the start codon to the stop codon encodes the complete polypeptide chain without the presence of a stop codon that interrupts translation. |
| 3' UTR | An untranslated sequence downstream of the coding region of mature messenger ribonucleic acid (mRNA). |
| PolyA tail | The role of the polyA tail is to maintain the activity of the mRNA as a translation template and to increase the stability of the mRNA itself. |
The cap structure is critical for mRNA. Existing studies have found that the 5' cap structure can regulate mRNA splicing and maturation, and help RNA transcripts pass through selective pores in the nuclear membrane and enter the cytoplasm. In addition, the 5' cap structure protects mRNA from exonuclease degradation, cooperates with translation initiation factor proteins, recruits ribosomes, and assists ribosome binding to mRNA, enabling translation to start from AUG.
Depending on the degree of methylation, mRNAs in nature can form 3 types of caps.
Cap 0: Guanosine is attached to the 5' end of the primary transcript by a 5'-5' pyrophosphate bond. When the 7th carbon atom of G is methylated to form m7GPPPN, the cap at this time is called Cap 0 and it exists in single cells.
Cap 1: If the 2'-O position of the first nucleotide of the transcript is also methylated, it forms m7GPPPNm, called Cap 1, which is the majority form of cap except for unicellular organisms. The main difference between Cap 0 and Cap 1 is whether the hydroxyl group at the 2'-O position of the first nucleotide is methylated or not.
Cap 2: If both the first and second nucleotide of the transcript are methylated at the 2'-O position, becoming m7G-PPPNmNm, it is called Cap2. Cap 2 is present in 10%-15% of eukaryotic cells.
Fig. 1 The 5′-end cap structure of eukaryotic mRNA (Ogino T,2011)
mRNA Capping is a vital service in RNA research and biotechnology, specifically focusing on the addition of a 5' cap structure to mRNA molecules. This modification plays a crucial role in protecting the mRNA from degradation, improving its stability, and enhancing translation efficiency for successful gene expression.
Improved mRNA Stability: mRNA capping significantly boosts the stability of mRNA by preventing its degradation by exonucleases. This ensures that mRNA remains intact and functional for longer durations, which is crucial in research and therapeutic applications like gene therapy.
Enhanced Translation Efficiency: The 5' cap structure enhances translation efficiency by facilitating the binding of ribosomes to the mRNA. This leads to higher protein yield in cellular processes, making it ideal for protein expression and vaccine development.
Facilitates mRNA Nuclear Export: Capped mRNA can efficiently exit the nucleus, which is essential for mRNA processing and translation. Without a proper cap, mRNA cannot be exported from the nucleus to the cytoplasm, hindering gene expression.
Vaccine and Gene Therapy Research: mRNA capping services are indispensable in the development of mRNA vaccines and gene therapies. By ensuring the stability and efficiency of the mRNA, these services maximize the effectiveness of mRNA-based therapeutic solutions.
At BOC Sciences, we offer a comprehensive suite of mRNA capping services designed to enhance the stability, translation efficiency, and overall functionality of your RNA-based projects. Our services include:
We provide custom 5' capping solutions to add the essential 7-methylguanosine (m7G) cap structure to your mRNA sequences. This modification is critical for preventing degradation and improving stability in both in vitro and in vivo environments.
For advanced applications, we offer Cap-1 and Cap-2 modifications, adding extra layers of capping for increased mRNA translation efficiency and enhanced immune evasion. These caps are commonly used in vaccines and therapeutic mRNA applications.
We provide full-length mRNA synthesis services with integrated capping, ensuring that your mRNA is properly modified from the 5' end to the 3' end. This service is ideal for clients working on protein expression or gene editing applications.
We offer comprehensive stability testing for capped mRNA molecules, ensuring that your modified mRNA maintains its integrity and function under different storage conditions. This service is crucial for applications that require long-term mRNA stability, such as gene therapy and vaccine development.
Our quality control (QC) processes ensure that the capped mRNA meets the highest standards of purity, functionality, and integrity. We offer detailed analytical testing, including gel electrophoresis, sequencing, and quantification to validate the quality of your mRNA product.
For researchers requiring large-scale mRNA production, we provide in vitro transcription (IVT) services with capping. This enables the generation of high-quality, capped mRNA for use in vaccines, therapeutic treatments, and research applications.
Our team of experts provides personalized consultation services for mRNA modification strategies, including capping, polyadenylation, and RNA purification. We help optimize your experimental protocols for maximum efficiency and reproducibility.
We ensure a streamlined and transparent process for delivering high-quality mRNA capping services. Our process is designed to meet the needs of researchers and developers at every stage of their projects.
We begin with an in-depth consultation to understand your project requirements. Whether you're developing mRNA vaccines, gene therapies, or protein expression systems, our team will tailor a capping strategy that aligns with your goals.
Once we understand your needs, we proceed with custom mRNA synthesis. Our experts design and synthesize the mRNA sequence, followed by the incorporation of the appropriate 5' cap structure (m7G, Cap-1, or Cap-2) based on your specific application.
We use in vitro transcription (IVT) technology to produce large quantities of capped mRNA. Our state-of-the-art transcription systems ensure that your mRNA is synthesized with high efficiency and quality, ready for downstream applications.
Before delivery, all capped mRNA undergoes rigorous quality control testing. This includes purity analysis, quantification, and functional validation to confirm that the capping process has been successfully completed and the mRNA is suitable for your research or therapeutic application.
Once testing is complete, we deliver the capped mRNA to your lab or facility. Our team provides continuous support throughout the usage phase, offering troubleshooting, optimization advice, and additional modifications as needed.
Our mRNA capping services offer several key advantages that set us apart in the industry, ensuring high-quality results for your research and development needs.
mRNA capping technology plays a crucial role in various cutting-edge fields. Our services help researchers and developers optimize their mRNA-based projects across a range of applications.
Capping is essential for mRNA vaccine development, as it enhances the stability and translation efficiency of the mRNA. This ensures effective antigen expression, leading to better immune responses in COVID-19 vaccines, cancer immunotherapies, and other infectious disease vaccines.
In gene therapy, mRNA capping improves the delivery and expression of therapeutic genes. Capped mRNA is more stable, efficiently translated, and able to enter the cell for targeted gene delivery, making it vital for treating genetic disorders like Cystic Fibrosis and muscular dystrophy.
mRNA capping boosts protein expression by ensuring that the mRNA remains stable and is efficiently translated in cells. This is widely used in research and industrial applications where high-quality recombinant proteins are required for drug discovery and biopharmaceutical production.
The high stability and translation efficiency provided by mRNA capping are critical for the development of mRNA-based diagnostic tests. These diagnostics are used in detecting viral infections, genetic diseases, and other medical conditions.
For CRISPR-Cas9 and other gene editing applications, mRNA capping ensures that the guide RNA and Cas9 proteins are properly expressed, improving the accuracy and efficiency of gene editing in therapeutic and research contexts.
mRNA capping technology is essential for cellular reprogramming and tissue engineering, where the mRNA guides the expression of specific genes required for tissue repair, stem cell differentiation, and organ regeneration.
mRNA capping is the addition of a 5' cap structure to the mRNA molecule, which protects the RNA from degradation, enhances stability, and promotes efficient translation. It is crucial for mRNA vaccines, gene therapy, and any application requiring robust mRNA function.
We offer various capping options, including the standard 7-methylguanosine (m7G) cap, as well as Cap-1 and Cap-2 modifications. These caps are selected based on the specific needs of your project, whether it's for mRNA vaccine development or gene therapy.
We conduct extensive quality control to ensure the capped mRNA meets the highest standards. This includes purity tests, functional assays, and quantification to confirm that the mRNA is ready for use in biotech applications and therapeutic research.
Yes, we offer high-scale mRNA production with capping included. Our state-of-the-art in vitro transcription systems ensure that we can produce large quantities of high-quality capped mRNA for applications like gene therapy and vaccines.
Absolutely! We offer custom mRNA capping services tailored to your specific research needs, whether you're working on gene therapy, protein expression, or vaccine development. We can help determine the best capping strategy for your project.
Yes, we offer mRNA purification services in addition to capping. Our purification protocols ensure that the capped mRNA is free from contaminants, allowing for optimal performance in research and therapeutic applications.
Get Started with Our mRNA Capping Services Today
Ready to enhance the stability and translation efficiency of your mRNA projects? Our expert team is here to support you with high-quality mRNA capping services tailored to your specific needs. Whether you're working on mRNA vaccines, gene therapy, or protein expression, we provide the solutions you need for successful outcomes.
Contact us today to schedule a consultation or get a custom quote for your project. Let's bring your mRNA research to the next level!
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