Relying on professional technology and advanced equipment, BOC Sciences focuses on the development of LNA probes to provide high-quality nucleic acid probe synthesis services and related product development. Our team has optimized the synthesis process, improved the production flow, and has the ability to produce from OD grade to gram scale to best meet our customers' needs.
LNA (locked nucleic acid) has a strong affinity for DNA, RNA and increased Tm value, thus allowing the design of probes with shorter sequences, increased sensitivity to single base mismatches, and application to SNP genotyping for the identification of 1-base differences. Compared to traditional nucleic acid probes, LNA probes have high specificity and sensitivity and are commonly used for the detection and analysis of DNA or RNA sequences. It can bind to target-specific sequences to improve the specificity of hybridization. This high specificity and sensitivity make LNA probes highly accurate in biological experiments such as detection of gene mutations, gene expression, and nucleic acid sequence analysis.
Fig 1. Main principle of DNA detection by short LNA/DNA capture probes. (Miotke et al., 2015)
When creating LNA probes, BOC Sciences researchers select nucleic acid sequences with high specificity and sensitivity, and then bind them to the lock nucleic acid. This binding allows the LNA probe to more accurately recognize the target nucleic acid sequence and better bind to it. We can provide customized oligo synthesis services according to the customer's design sequence or LNA modification requirements, such as modification sites, quantity, and phosphorylation needs.
The LNA probe parameters we can provide include the following.
| Item | Specifications |
| LNA bases | Multiple LNA bases can be added to a single sequence. |
| Dyes available | FAM, TET, CAL Fluor Gold 540, CIV-550, JOE, HEX, CAL Fluor Orange 560, Quasar 570, Cy3, TAMRA, CAL Fluor Red 590, ROX, CAL Fluor Red 610, CAL Fluor Red 635, Cy5, Quasar 670, Quasar 705 |
| Yield | 5, 20 or 50 nmols delivered |
| Delivery format | Dry or solution |
| Purity | HPLC test purity ≥95%, customers can also customize to set higher purity standards. |
| Turnaround time | The estimated time for you to get your target product is 5 business days. |
If you have a request for oligonucleotide synthesis, please feel free to contact us.
The locked ribose structure in LNA probes provides enhanced binding affinity and thermal stability, allowing for shorter probe sequences with improved mismatch discrimination capabilities.
Optimal LNA placement depends on sequence composition, target accessibility, and desired melting temperature, with strategic positioning typically at probe ends or surrounding critical bases for maximum specificity enhancement.
Yes, LNA technology enables design of multiple probes with distinct melting temperatures, making them ideal for multiplex assays where precise temperature control and specific hybridization are required.
All LNA probes undergo rigorous HPLC purification to achieve ≥95% purity, with mass spectrometry verification and functional validation to guarantee optimal hybridization performance.
LNA incorporation accelerates hybridization rates while maintaining stringent specificity, enabling faster assay times and improved signal-to-noise ratios in detection applications.
Reference
