Custom tRNA Synthesis

Transfer RNA (tRNA) is a dynamic molecule that is a key component of the translation apparatus of nearly all life forms. The typical length of tRNA is 76 to 90 nucleotides. tRNAs help decode messenger RNA (mRNA) sequences into proteins. During translation, tRNAs function at specific sites in the ribosome. tRNAs are involved in epigenetic regulation of germ cells, and modify enzyme mutations that are associated with human disease. Therefore, comprehensive understanding of the structure and functions of tRNA plays an important role in the human diseases research. As a leading bio-commercial company, BOC Sciences provides any length of tRNA according to different project requirements of customers.

Roles of tRNA in Gene Expression.Fig 1. Roles of tRNA in Gene Expression. (Raina M, 2014)

Our tRNA Synthesis Services

Custom tRNA Synthesis with Unnatural Amino Acids (UAAs)

UAAs incorporation can selectively activate, inhibit, or reversibly regulate target homologs. Genetically integrated UAAs can regulate protein function through translational, optical, or chemical control. Incorporation of UAA into tRNAs can endow novel structural and functional features on polypeptide sequences, thereby enabling ribosomes to synthesize highly modified drug-like peptides. BOC Sciences provides enzymatic aminoacylation service of tRNA, through site-specific incorporation of UAA into tRNA, enabling site-specific incorporation of many structurally and chemically distinct UAAs.

Custom Specially Modified tRNA Synthesis

tRNA modification plays an important role in many cellular processes, and chemically modified tRNAs are powerful tools for studying the field of RNA biology. Developing new methods and technologies to modify RNA could have profound impacts in the fields of animal health, plant science and therapeutics. BOC Sciences provides customized specially modified tRNA synthesis service, which can be used to generate stable modified tNRA. The first method is accomplished by utilizing an enzymatic reaction, and the second one is developed based on solid-phase synthesis and click chemistry. Furthermore, to verify the completeness of the modification, our experts offer a comprehensive mapping service of tRNA modifications using affinity chromatography techniques and high-throughput tRNA sequencing.

Available Chemical Modification

We offer you a wide range of chemical modifications to provide you with custom tRNA synthesis services. At BOC Sciences, available chemical modification options include: 1-methyladenosine, 5-methylcytidine, 1-methylpseudouridine, 5-methyluridine, 2'-O-methylguanosine, 5,6-di Hydrogen uridine, 2'-O-methylcytidine, inosine, 4-thiouridine, pseudouridine, etc.

BOC Sciences Synthesis Standard

All synthetic tRNA can be applied in many fields, thanks to the powerful advantages of BOC Sciences' synthesis technology:

  • High reproducibility.
  • Unparalleled purity without additional purification time and expense.
  • Enhanced maximum sequence fidelity and integrity.
  • Allows for the fastest and mildest deprotection conditions, facilitating the highest purity levels of synthetic tRNA on the market today.

Custom Synthesis Scales

Aiming to reduce costs and run discovery projects with smaller amounts of test material, BOC Sciences offers reduced-scale synthesis services. Each tRNA molecule has unique synthetic challenges depending on different application, compatibility, sequence, length, and modification position. You can rely on our experts to aid your custom tRNA designs and support your experimental goals.

Features of Our Custom tRNA Synthesis Services

  • Industry-leading experimental capabilities.
  • Expertise in synthetic chemistry.
  • Extensive chemical modification options for tRNA synthesis.
  • Synthesis capability of high-yield single strand RNA.
  • Delivery of high-performance tRNAs with proprietary modifications.


  1. Raina M; et al. tRNAs as regulators of biological processes. Front Genet. 2014 Jun 11; 5: 171.
* Only for research. Not suitable for any diagnostic or therapeutic use.
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