BOC Sciences has the expertise and capability to provide oligonucleotide modification services to clients worldwide. BOC Sciences' state-of-the-art facilities, advanced technology and experienced staff can provide you with a full range of splicing and chemical modifications to meet your biological, diagnostic and drug discovery needs.
Splicing refers to the editing of newly transcribed pre-messenger RNA (pre-mRNA) by removing introns (non-coding regions of the gene) and adding or joining exons (coding regions). Mature mRNA is thus created, which is then used as a template for the synthesis of specific proteins.
SSOs are short, synthetic, antisense, modified nucleic acids that base pair with pre-mRNA and disrupt the normal splicing repertoire of transcripts by blocking protein-RNA binding interactions between RNA-RNA base pairing or splicing units and pre-mRNA. SSOs support an effective and specific way to therapeutically target and alter splicing. Modifications in SSOs involve altering the phosphate backbone and/or sugar component of the oligonucleotide and adding chemical modifications aimed at altering splicing rather than causing degradation of the bound pre-mRNA.
|Phosphorodiamidate morpholino (PMO) oligo modification||PMO has a morpholine ring to replace the furanose ring in native nucleic acids and a neutral phosphodiester backbone to replace the negatively charged phosphodiester backbone. The neutral charge of PMO results in low binding to plasma proteins, which improves tolerance in vivo.||Inquiry|
|2'-O-methyl-phosphorothioate (2'-OMePS) oligo modification||The 2'-hydroxyl group of the 2'-OMePS ribose ring is replaced by a 2'-O-2-methoxyethyl and phosphate linkage. This modification makes it more stable in vivo, less susceptible to breakdown by nucleases, and still maintains its base-pairing function.||Inquiry|
|LNA/ Amido-bridged nucleic acid (AmNA)/ Guanidine-bridged nucleic acid (GuNA) modification||LNA is a nucleotide analogue carrying an altered ribose in which a methylene bridge links the 2'-O to the 4'-C atom in the furanose ring. This bridge allows LNA to form a strict N-type conformation, thus promoting binding affinity to complementary RNAs. Both AmNA-modified SSO and GuNA-modified SSO exhibited higher exon skipping activity and efficient splicing regulation.||Inquiry|