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BOC Sciences has the expertise and capability to provide oligonucleotide modification services to clients worldwide. BOC Sciences' state-of-the-art facilities, advanced technology and experienced staff can provide you with a full range of splicing and chemical modifications to meet your biological, diagnostic and drug discovery needs.
What is Oligo Splicing and Chemical Modification?
Splicing
Splicing refers to the editing of newly transcribed pre-messenger RNA (pre-mRNA) by removing introns (non-coding regions of the gene) and adding or joining exons (coding regions). Mature mRNA is thus created, which is then used as a template for the synthesis of specific proteins.
Splice-switching oligonucleotides (SSOs)
SSOs are short, synthetic, antisense, modified nucleic acids that base pair with pre-mRNA and disrupt the normal splicing repertoire of transcripts by blocking protein-RNA binding interactions between RNA-RNA base pairing or splicing units and pre-mRNA. SSOs support an effective and specific way to therapeutically target and alter splicing. Modifications in SSOs involve altering the phosphate backbone and/or sugar component of the oligonucleotide and adding chemical modifications aimed at altering splicing rather than causing degradation of the bound pre-mRNA.
Our Oligo Splicing and Chemical Modification Services Specification
| Modification | Description | Price |
| Phosphorodiamidate morpholino (PMO) oligo modification | PMO has a morpholine ring to replace the furanose ring in native nucleic acids and a neutral phosphodiester backbone to replace the negatively charged phosphodiester backbone. The neutral charge of PMO results in low binding to plasma proteins, which improves tolerance in vivo. | Inquiry |
| 2'-O-methyl-phosphorothioate (2'-OMePS) oligo modification | The 2'-hydroxyl group of the 2'-OMePS ribose ring is replaced by a 2'-O-2-methoxyethyl and phosphate linkage. This modification makes it more stable in vivo, less susceptible to breakdown by nucleases, and still maintains its base-pairing function. | Inquiry |
| LNA/ Amido-bridged nucleic acid (AmNA)/ Guanidine-bridged nucleic acid (GuNA) modification | LNA is a nucleotide analogue carrying an altered ribose in which a methylene bridge links the 2'-O to the 4'-C atom in the furanose ring. This bridge allows LNA to form a strict N-type conformation, thus promoting binding affinity to complementary RNAs. Both AmNA-modified SSO and GuNA-modified SSO exhibited higher exon skipping activity and efficient splicing regulation. | Inquiry |
Why Choose Oligo Splicing and Chemical Modification Service from BOC Sciences?
- High-quality products
- competitive price
- Data analysis, detailed reporting including results and discussion
- Guaranteed total yield per oligonucleotide is a fixed final yield of OD units
- All samples are carefully monitored for stability and characterized to ensure batch-to-batch consistency
References
- Roberts, T.C.; et al. Advances in oligonucleotide drug delivery. Nat Rev Drug Discov. 2020. 19(10): p. 673-694.
- Nan, Y; et al. Antisense Phosphorodiamidate Morpholino Oligomers as Novel Antiviral Compounds. Front Microbiol. 2018. 9: p. 750.
- Pires, V.B.; et al. Short (16-mer) locked nucleic acid splice-switching oligonucleotides restore dystrophin production in duchenne muscular dystrophy myotubes. PLoS One. 2017. 12(7): p. e0181065.
- Shimo, T.; et al. Design and in vitro evaluation of splice-switching oligonucleotides bearing locked nucleic acids, amido-bridged nucleic acids, and guanidine-bridged nucleic acids. Int J Mol Sci. 2021. 22(7).
* Only for research. Not suitable for any diagnostic or therapeutic use.
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