Custom Minor Groove Binder (MGB) Probe Synthesis

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BOC Sciences draws on our expertise and technology to offer a wide range of high-quality diagnostic probes to meet the needs of research and industry from R&D to production.

What are Minor Groove Binder (MGB) Probes?

MGB (Minor Groove Binder) probes are specialized probes for nucleic acid detection and are widely used in nucleic acid amplification techniques such as real-time polymerase chain reaction (real-time PCR). MGB probes are commonly used to detect and measure the presence of target DNA or RNA sequences and are useful for enhancing the specificity and sensitivity of PCR.

Structure and mechanism of action of MGB-probesFig 1. Structure and mechanism of action of MGB-probes. (Navarro et al., 2015)

Advantages of MGB Probes

BOC Sciences' MGB Probe Customization Service

The design and synthesis of MGB probes are based on the DNA hybridization chain reaction (HCR), a signal amplification technique based on DNA's catalytic mechanism, in which two DNA single strands of a probe are designed and synthesized to hybridize with each other to form a stem-and-loop structure that triggers the subsequent amplification of the signal. MGB probes, on the other hand, are based on HCR, which adopts a four-stranded structure for the stem of the single strand of probe DNA, thus improving the stability and specificity of the probes.

However, the following factors need to be considered when performing the design process of MGB probes.

With our unique supply capabilities, BOC Sciences' laboratories can provide oligonucleotide synthesis with consistent quality at every stage of development. By manufacturing our dye phosphoramidites, CPGs, linker phosphoramidites, etc., we can ensure that projects are scaled up from inception to commercial scale.

Reference

  1. Navarro E, et al. Real-time PCR detection chemistry[J]. Clinica chimica acta, 2015, 439: 231-250.
* Only for research. Not suitable for any diagnostic or therapeutic use.
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