Custom RNA Oligonucleotides Synthesis

Custom RNA Oligonucleotides Synthesis

BOC RNA provides custom DNA synthesis services to help customers save time and money. All customized DNA oligonucleotides are produced under strict quality control standards, and all synthetic fragments are verified to ensure 100% sequence identity.

Introduction to RNA Oligonucleotides

  • What are RNA Oligos

RNA Oligonucleotides are short nucleotide sequences of RNA that have gained immense popularity in the scientific community due to their ability to modulate gene expression, silence genes, and even target viral infections.

  • How to Synthesize RNA Oligos

The synthesis of RNA oligonucleotides is a crucial step that determines the purity, length, and stability of the oligonucleotides. RNA oligonucleotides are usually synthesized using chemical or enzymatic methods. The chemical synthesis method involves building the RNA sequence one nucleotide at a time, typically in a solid-phase synthesis format. Enzymatic methods, on the other hand, use RNA polymerases to incorporate nucleotides onto the growing RNA oligonucleotide chain.

  • What are the Applications of RNA Oligos

Single-stranded RNA can be used forCustom microRNA inhibitors
Aptamer
Ribozyme
Ribonucleic acid
FISH / ISH probe
FRET probe
RNA/DNA hybrid Custom RNA Duplex Dicer substrate
Antisense oligonucleotide Enzyme substrate
Long RNA oligonucleotides can be used forSingle guide RNA for CRISPR genome editing
RNA-based diagnosis and treatment method development
  • Delivery and Stability of RNA Oligos

One of the primary challenges of using RNA oligonucleotides is their delivery and stability in vivo. RNA oligonucleotides are vulnerable to degradation by nucleases and face additional challenges when attempting to cross cell membranes. Different delivery strategies, such as encapsulation in nanoparticles or chemical modifications, such as phosphorothioate modification, have been explored to enhance the delivery and stability of RNA oligonucleotides in vivo.

Custom Synthesis of RNA Oligos

BOC Sciences provides a wide range of RNA synthesis according to specific requirements. To ensure the highest quality of RNA oligonucleotides, they are synthesised using the latest nucleic acid technology and identified by mass spectrometry to determine the final sequence and purity.

  • Types of RNA Oligos Synthesis

Service CategoryDescription
Custom miRNA mimics SynthesismiRNA mimics are the mimics of endogenous miRNA. miRNA mimics are chemically synthesized mature miRNA duplexes that enhance the function of endogenous miRNA.Inquiry
Custom miRNA Inhibitor SynthesismiRNA inhibitors are chemically modified to target miRNA in cells specifically.Inquiry
Custom miRNA Agomir SynthesismiRNA agomir is a specially labeled and chemically modified double-stranded small RNA (miRNA agonist) that regulates the biological functions of target genes by mimicking endogenous miRNA into the miRiSC complex.Inquiry
Custom miRNA Antagomir SynthesismiRNA antagomir is a small single-stranded RNA (miRNA antagonist) designed according to the mature microRNA sequence and chemically prepared with special labeling and chemical modifications.Inquiry
Custom microRNA Lentivirus Packaging ServiceAt BOC Sciences, we offer a comprehensive Custom MicroRNA Lentivirus Packaging Service to support researchers in their gene modulation studies. Our service includes the construction of microRNA overexpression/inhibition lentivector clones and the packaging of lentivirus for transduction experiments.Inquiry
Custom miRNA Sponges SynthesismiRNA sponges, also known as competitive endogenous RNA (ceRNA) molecules, are synthetic transcripts containing multiple binding sites for a specific miRNA.These sponges function as competitive inhibitors by sequestering miRNAs and preventing them from binding to their target mRNAs, thus derepressing the expression of miRNA-regulated genes.Inquiry
Custom ssRNA SynthesisSingle-stranded RNA (ssRNA) is an RNA molecule containing only one strand. Unlike double-stranded DNA, most RNAs in organisms are single-stranded molecules.Inquiry
Custom dsRNA SynthesisDouble-stranded RNA (dsRNA) is a unique form of RNA. dsRNAs are usually shorter, possess enhanced structural instability due to uncertainties, and are highly influenced by environments.Inquiry
  • Optional Service - Modifications/Labeling of RNA Oligos

BOC RNA provides various modifications, includes regular modification, label modification, and backbone modification.

ClassificationModificationScale
PhosphorothioatesSPO3
  • 20 nmol
  • 50 nmol
  • 100 nmol
  • 200 nmol
  • 1 µmol
  • 5 µmol
  • 10 µmol
  • more
Digoxin3' or 5' Digoxin
Quenchers3' DABCYL
ElectrochemicalFerrocene
Modified Bases
  • dI
  • dU
Phosphorylation
  • 5' PO4
  • 3' PO4
Disulfide Linkers
  • 3' C3 S-S
  • 5' C6 S-S
Amino Linkers
  • 3' or 5' NH2 C3
  • 5' NH2 C6
  • 3' NH2 C7
  • 5' NH2 C12
  • NH2 C6 dT
Spacers
  • Spacer C3
  • Spacer C6
  • Spacer 9
  • Spacer 18
  • dSpacer
Biotin
  • 5' Biotin
  • 3' Biotin
  • 5' Biotin TEG
  • 3' Biotin TEG
  • 5' Dual Biotin
  • 5' or Internal Biotin dT
  • 5' PC Biotin
Fluorophores
  • 5' Cy3
  • 5' Cy5
  • 5' FAM
  • 3' FAM
  • 5' HEX
  • 3' TET
  • 3' or 5' TAMRA
  • Internal TAMRA

Custom RNA Oligonucleotides Synthesis

Please contact us for purification advice or quotation.

* Only for research. Not suitable for any diagnostic or therapeutic use.
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