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BOC RNA provides custom DNA synthesis services to help customers save time and money. All customized DNA oligonucleotides are produced under strict quality control standards, and all synthetic fragments are verified to ensure 100% sequence identity.
Introduction to RNA Oligonucleotides
What are RNA Oligos
RNA Oligonucleotides are short nucleotide sequences of RNA that have gained immense popularity in the scientific community due to their ability to modulate gene expression, silence genes, and even target viral infections.
How to Synthesize RNA Oligos
The synthesis of RNA oligonucleotides is a crucial step that determines the purity, length, and stability of the oligonucleotides. RNA oligonucleotides are usually synthesized using chemical or enzymatic methods. The chemical synthesis method involves building the RNA sequence one nucleotide at a time, typically in a solid-phase synthesis format. Enzymatic methods, on the other hand, use RNA polymerases to incorporate nucleotides onto the growing RNA oligonucleotide chain.
What are the Applications of RNA Oligos
| Single-stranded RNA can be used for | Custom microRNA inhibitors Aptamer Ribozyme Ribonucleic acid FISH / ISH probe FRET probe RNA/DNA hybrid Custom RNA Duplex Dicer substrate Antisense oligonucleotide Enzyme substrate |
| Long RNA oligonucleotides can be used for | Single guide RNA for CRISPR genome editing RNA-based diagnosis and treatment method development |
Delivery and Stability of RNA Oligos
One of the primary challenges of using RNA oligonucleotides is their delivery and stability in vivo. RNA oligonucleotides are vulnerable to degradation by nucleases and face additional challenges when attempting to cross cell membranes. Different delivery strategies, such as encapsulation in nanoparticles or chemical modifications, such as phosphorothioate modification, have been explored to enhance the delivery and stability of RNA oligonucleotides in vivo.
Custom Synthesis of RNA Oligos
BOC Sciences provides a wide range of RNA synthesis according to specific requirements. To ensure the highest quality of RNA oligonucleotides, they are synthesised using the latest nucleic acid technology and identified by mass spectrometry to determine the final sequence and purity.
Types of RNA Oligos Synthesis
| Service Category | Description | |
| Custom miRNA mimics Synthesis | miRNA mimics are the mimics of endogenous miRNA. miRNA mimics are chemically synthesized mature miRNA duplexes that enhance the function of endogenous miRNA. | Inquiry |
| Custom miRNA Inhibitor Synthesis | miRNA inhibitors are chemically modified to target miRNA in cells specifically. | Inquiry |
| Custom miRNA Agomir Synthesis | miRNA agomir is a specially labeled and chemically modified double-stranded small RNA (miRNA agonist) that regulates the biological functions of target genes by mimicking endogenous miRNA into the miRiSC complex. | Inquiry |
| Custom miRNA Antagomir Synthesis | miRNA antagomir is a small single-stranded RNA (miRNA antagonist) designed according to the mature microRNA sequence and chemically prepared with special labeling and chemical modifications. | Inquiry |
| Custom microRNA Lentivirus Packaging Service | At BOC Sciences, we offer a comprehensive Custom MicroRNA Lentivirus Packaging Service to support researchers in their gene modulation studies. Our service includes the construction of microRNA overexpression/inhibition lentivector clones and the packaging of lentivirus for transduction experiments. | Inquiry |
| Custom miRNA Sponges Synthesis | miRNA sponges, also known as competitive endogenous RNA (ceRNA) molecules, are synthetic transcripts containing multiple binding sites for a specific miRNA.These sponges function as competitive inhibitors by sequestering miRNAs and preventing them from binding to their target mRNAs, thus derepressing the expression of miRNA-regulated genes. | Inquiry |
| Custom ssRNA Synthesis | Single-stranded RNA (ssRNA) is an RNA molecule containing only one strand. Unlike double-stranded DNA, most RNAs in organisms are single-stranded molecules. | Inquiry |
| Custom dsRNA Synthesis | Double-stranded RNA (dsRNA) is a unique form of RNA. dsRNAs are usually shorter, possess enhanced structural instability due to uncertainties, and are highly influenced by environments. | Inquiry |
Optional Service - Modifications/Labeling of RNA Oligos
BOC RNA provides various modifications, includes regular modification, label modification, and backbone modification.
| Classification | Modification | Scale |
| Phosphorothioates | SPO3 |
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| Digoxigenin | 3' or 5' Digoxigenin | |
| Quenchers | 3' DABCYL | |
| Electrochemical | Ferrocene | |
| Modified Bases |
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| Phosphorylation |
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| Disulfide Linkers |
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| Amino Linkers |
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| Spacers |
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| Biotin |
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| Fluorophores |
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Please contact us for purification advice or quotation.
* Only for research. Not suitable for any diagnostic or therapeutic use.
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GMP Oligonucleotide Manufacturing Service
For more than 15 years, BOC Sciences has been manufacturing oligonucleotides for pre-clinical, pharmaceutical, food safety, and animal health industries. We provide customized and flexible oligonucleotide GMP or non-GMP production services to meet different production needs.
Lipid Nanoparticle(LNP) for RNA Delivery
BOC Sciences offers comprehensive LNP- mRNA delivery services tailored to meet the specific needs of mRNA vaccine development. Our expertise in nanoparticle formulation and mRNA chemistry enables us to design custom LNP formulations optimized for stability, efficacy, and safety.
BOC Sciences promises to offer you with GalNAc-siRNA conjugation services to help you conduct further research on GalNAc-siRNA conjugates and explore their mores omnics capabilities, the working mechanism as well as their potential therapeutic profiles.
BOC Sciences offers aptamer customization services to generate high-quality aptamers tailored to your goals, delivering excellent results even for the most difficult target molecules.
Peptide-Oligonucleotide Conjugation
BOC Sciences is committed to providing our customers with comprehensive modification and labeling, offering affordable custom oligonucleotides or peptide-oligonucleotide conjugates.
