Biotin Labeling of Oligonucleotides

What is Biotin Labeling?

Biotin labeling is an analytical tool to label target molecules (e.g., proteins, nucleic acids, small molecules, etc.) by introducing biotin into these molecules for subsequent study for experimental purposes of detection, purification, or visualization. Biotin is a water-soluble derivative of vitamin B that binds with high affinity and specificity to avidin or streptavidin. Thus, by introducing biotin into a target molecule, the detection and isolation of labeled molecules can be achieved by exploiting the high affinity and specificity of biotin binding to avidin or streptavidin.

Figure 1. Biotin as a reactive handle to selectively label proteins and dna with small molecules. (A, D, Cotton.; et al, 2022)

The steps of biotin labeling usually include:

• Introduction of the biotin marker: attachment of the biotin to the target molecule. This can be achieved by various methods, such as chemical conjugation of biotin compounds and genetic engineering techniques.
• Visualization of the assay: The biotin-introduced marker molecule binds with high affinity to affinity proteins or streptavidin. The marker can then be used for detection, purification or other experimental purposes. Common assays include enzyme-linked immunosorbent assay (ELISA), fluorescence microscopy, and Western blotting. All of these methods utilize the highly specific binding between biotin and affinities.

What is Biotin Oligonucleotide?

Biotin oligonucleotide is a biotin-labeled oligonucleotide molecule. Oligonucleotides are molecules consisting of shorter sequences of nucleotides, and in Biotin Oligonucleotide, one or more positions of these oligonucleotides are labeled with biotin. Biotin Oligonucleotide is commonly used in molecular biology and biochemistry experiments where the affinity of biotin can be used to label and detect these molecules. This labeling is highly specific and sensitive and is therefore widely used in a variety of applications.

Figure 2. Site-specific attachment of DNA handles to a protein is necessary for force spectroscopy using a double-dumbbell optical tweezers set up. (S, Marie.; et al, 2019)

Applications of Biotin Oligonucleotide

• Affinity Purification: The powerful binding between Biotin and Biotin can be used for affinity purification of oligonucleotides, resulting in rapid and efficient isolation and purification of target oligonucleotides.
• In Situ Hybridization: Biotin oligonucleotide can be used in in situ hybridization experiments where the oligonucleotide binds to a complementary nucleic acid in a biological sample, which is detected by the affinity of the biotin.
• Northern Blot: Biotin oligonucleotide is used as a labeling probe in Northern blot experiments to detect and analyze specific nucleotide sequences in RNA samples.
• Real-time PCR: In real-time PCR, biotin oligonucleotide can be combined with a fluorescent marker to quantify the presence of the target nucleotide by monitoring the fluorescent signal during the PCR process in real-time.
• Enzyme-linked immunosorbent assay (ELISA): Biotin oligonucleotide can be used in ELISA assays to detect target molecules through the binding of biotin to enzyme-labeled affinity factors.

BOC Sciences Offers Biotin Labeling Oligonucleotides

BOC Sciences provides you with biotin-based labeled oligonucleotides to meet your customization needs in a variety of biological and laboratory applications in assays, imaging, and quantification that require nucleic acid analysis. We offer the following types of biotin labeling customization needs for you to choose from, in addition to oligonucleotides, which are equally suitable for labeling of nucleotides, primers, PCR amplicons, and aptamers.

Advantages of BOC Sciences' Biotin Labeling Oligonucleotides

• Highly flexible and customizable: BOC Sciences can design and synthesize oligonucleotides with specific sequences and modifications according to customers' needs.
• Technical support and consulting services: BOC Sciences provides technical support and consulting services on the use of biotin-modified oligonucleotides to help researchers select appropriate labeling strategies and experimental designs.
• Oligonucleotides market competitive price: BOC Sciences focuses on providing you with high performance products and services.
• Quality control, cGMP service: Oligonucleotides from BOC Sciences have high purity and high quality, which is the reason why we are widely praised.

Based on industry-leading oligonucleotide synthesis technology platforms and state-of-the-art equipment, more tailor-made services not mentioned are available at BOC Sciences. Please relax and contact us, we always look forward to partnering with you.

References

1. A, D, Cotton.; et al. Biotin as a Reactive Handle to Selectively Label Proteins and DNA with Small Molecules. ACS Chemical Biology. 2022, 17(12), 3270-3275.
2. S, Marie.; et al. Bioorthogonal Protein-DNA Conjugation Methods for Force Spectroscopy. Scientific Reports. 2019, 9: 13820.

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Biotin Labeling of Oligonucleotides

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