Isotope labeling is the introduction of isotopes into the structure of a molecule for the purpose of tracing, studying, or labeling specific properties of the molecule. Isotopes are similar elements with the same number of nuclei in an atom but different masses, and have almost identical chemical properties. By introducing isotopes, it is possible to gain a deeper understanding of a molecule's behavior by determining its involvement in a reaction. Common isotopes include hydrogen isotopes (deuterium and hydrogen), carbon isotopes (12C and 13C), oxygen isotopes (16O and 18O), nitrogen isotopes (14N and 15N), and phosphorus isotopes (31P and 32P).
A class of atoms with the same number of protons and different numbers of neutrons in the nucleus are called isotopes, which are in the same position on the periodic table and can be categorized into stable and radioactive isotopes. The nucleus of a radioactive isotope is unstable, and it decays into another isotope by spontaneously emitting particles. The isotopes that are not radioactive are called stable isotopes, part of which are stable products formed by the decay of radioactive isotopes, called radiogenic isotopes, and part of which are natural stable isotopes that have remained stable since nuclear synthesis. Stable isotopes are safer, non-polluting and easier to control than radioisotopes, and are widely used in research in geology, ecology, medicine, agriculture and other fields.
Isotope labeling is a technique commonly used in molecular research involving the introduction of isotopes into the structure of molecules in order to trace, label, or study the properties, metabolic pathways, and interactions of those molecules. In nucleic acid research, isotope labeling is commonly used to track the biological behavior of DNA or RNA molecules.
Figure 1. Stable isotope labeling methods for DNA. (H, T, Frank.; et al, 2016)
BOC Sciences is at the forefront of providing highly specialized and customizable isotope-labeled oligonucleotide services to meet the diverse needs of researchers and industries engaged in cutting-edge molecular research. Our isotope-labeled oligonucleotides are meticulously designed and synthesized to be doped with stable isotopes, enabling researchers to achieve unparalleled precision in their studies.
BOC Sciences offers the following isotope labeling services.
By choosing BOC Sciences, you gain a reliable and innovative partner dedicated to advancing your research through high-quality isotope-labeled oligonucleotides. Based on industry-leading oligonucleotide synthesis technology platforms and state-of-the-art equipment, more tailor-made services not mentioned are available at BOC Sciences. Please relax and contact us, we always look forward to partnering with you.
Isotope labeling involves incorporating isotopes, such as 13C, 15N, or 32P, into oligonucleotides. This technique is used to track molecular interactions, metabolic pathways, and structural characteristics in DNA or RNA studies, providing deeper insights into biological and chemical processes.
Stable isotopes have a stable nucleus and do not decay, making them safer and easier to handle than radioactive isotopes. Stable isotopes, like 13C and 15N, are commonly used in molecular studies for NMR analysis and other research applications because they are non-radioactive and easier to control.
Isotope-labeled oligonucleotides are used in metabolomics, protein interaction studies, and molecular structure research. They enable precise tracking of DNA, RNA, and protein behaviors in various biological processes.
Isotope labeling improves the clarity of NMR spectra by replacing natural isotopes with stable ones like 13C or 15N. This helps researchers analyze molecular structures and interactions with greater precision.
Yes, isotope labeling allows for the tracing of nucleic acid synthesis by incorporating isotopes like 3H thymine into DNA. This helps researchers monitor the formation and degradation of nucleic acids over time.
BOC Sciences offers a range of isotopes including 32P, 33P, 15N, and 13C for oligonucleotide labeling. Each isotope serves specific applications like NMR, protein interaction studies, and mass spectrometry.
Isotope labeling allows researchers to track protein interactions with nucleic acids or other molecules. This enhances the study of binding affinities and molecular mechanisms involved in cellular processes.
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