Oligo Phosphorylation Modification

With advanced oligonucleotide modification technology and experienced staff, BOC Sciences provides high-quality phosphorylation-modified oligonucleotide synthesis services. We aspire to be your trusted preferred service provider.

Introduction

Phosphorylation, a vital modification in biochemistry and molecular biology, is a chemical reaction that adds a phosphate group (PO^3-) to an organic molecule. Oligonucleotide phosphorylation plays an important role in oligonucleotide modification, facilitating researchers to further investigate the structure and function of DNA and RNA oligonucleotides and apply nucleic acid molecules in the fields of medicine and genetic engineering.

• 3' Phosphate

PCR extension of oligonucleotides requires the presence of a 3'-hydroxyl group (3'-OH). Any stable chemical modification of the 3'-terminus will prevent PCR amplification (i.e., act as a PCR blocker), and the addition of a phosphate group at the 3'-terminus is often selected. This modification inhibits the degradation of certain 3'-exons and can be used to block the extension of DNA polymerase.

Installing a 3'-phosphate group as a protecting group has many advantages because it is not a bulky hydrophilic group, is hydrolytically stable during storage and synthesis, ensures recognition of the substrate by polymerase without affecting its incorporation into DNA, and can be easily removed in the presence of phosphatase. Moreover, considering that phosphorylated oligonucleotides can be used to modify the sensitivity of the sequence to exonuclease hydrolysis, the 3' phosphate is required for fluorescence resonance energy transfer (FRET) probes labeled with receptor fluorophores.

• 5' Phosphate

Oligonucleotides require 5' phosphate for ligation. Oligonucleotides containing 5'-phosphate groups have been used for various purposes in molecular biology: e.g., as linkers and adapters, for cloning and gene construction, and ligase chain reactions.

• 5' Triphosphate

5'-triphosphate (5'-PPP) modifications of DNA or RNA oligonucleotides have become biologically important for biochemical and therapeutic applications. They have potential antiviral and anticancer properties and can lead to stimulation of immune responses. They are substrates for polymerase chain reactions, nucleic acid ligation reactions for structural and mechanistic studies, and intermediates for m7G-enzymatic synthesis, where 5'-PPP-capped RNAs are common materials for mRNA maturation.

• 2',3'-cyclic phosphate oligos

2',3'-cyclic phospho-oligonucleotides are intermediates of ribonuclease-catalyzed ribonuclease hydrolysis, and they are ribonuclease substrates. It may play an important role in regulating biological processes and thus has been studied as a model substrate for various ribonuclease kinetic analyses.

• Diphosphosphate

Diphosphorylated RNA, a novel discovered modified state naturally occurring in Escherichia coli, is the preferred substrate for the RNA pyrophosphohydrolase RppH and has a dramatic impact on mRNA lifespan. Recently, 5'-fluorodiphosphate-labeled oligonucleotide probes have been developed for the study of nucleic acid secondary structure and DNA-protein interactions.

Our Oligo Phosphorylation Modification Services Specification

Why choose BOC Sciences?

BOC Sciences' advanced technology and experienced staff are dedicated to providing phosphorylation-modified oligonucleotide synthesis to improve your process and reduce overall development time.

• All operations are carried out under strict manufacturing and QC & QA standards
• A wide range of purification methods, specifications and delivery formats are available, and flexible synthesis scales are available upon customer request
• Provide a one-stop service from design to purification and delivery to customers
• Discussions with our partner customers to determine their specific requirements and expectations to meet partner requirements
• Using appropriate techniques, diverse therapeutic expertise, makes studies shorter and more efficient