Cell-Penetrating Peptide-Oligonucleotide Conjugation

BOC Sciences focuses on advancing drug delivery development through bioconjugation. Relying on our state-of-the-art manufacturing facilities and comprehensive experience in conjugation technologies, BOC Sciences offers conjugation services to combine oligonucleotides with cell-penetrating peptides(CPPs). We have the capability and confidence to meet your needs for novel conjugates.

Introduction of Cell-Penetrating Peptide(CPP)-Oligonucleotide Conjugation

• Overview of CPP

Cell-penetrating peptide (CPP), also known as protein transduction domain (PTD), is a short peptide that plays an important role in intercellular material transport. CPP is capable of entering cells and transporting various types of molecules, such as proteins, nucleic acids and nanoparticles across cell membranes.

Figure 1. Glycosylated cell-penetrating peptides(GCPPs).( Gallego, I; et al, 2019)

• Application of CPP-Oligonucleotide Conjugation

The main role of CPP in CPP-oligonucleotide conjugation is to enhance the cellular uptake and intracellular delivery of oligonucleotides, thus improving their bioavailability and efficacy, ultimately leading to enhanced therapeutic or imaging effects.

• Drug Delivery: Promotion of target cell uptake
• Gene Therapy: Enhanced gene silencing effect
• Antimicrobial Therapy: Improved bacterial penetration
• Targeted Therapy: Selective and precise treatment
• Diagnostics & Imaging: Real-time imaging

Introduction of CPP-Oligonucleotide Conjugation Services

• What Are Our CPP-Oligonucleotide Conjugation Services?

1. CPP-Oligonucleotide Conjugation Synthesis: The preparation of CPP-oligonucleotide conjugates usually involves two main steps. The first is the synthesis or acquisition of CPP and oligonucleotides, followed by the selection of a suitable chemical coupling strategy to couple them together. BOC Sciences offers the following coupling strategies for the synthesis of CPP-Oligonucleotide Conjugation.

• Covalent Coupling: Reactive groups on CPPs (e.g. lysine residues) and activating groups on oligonucleotides (e.g. maleimide) can be used for coupling.
• Disulfide Bond Formation: CPP containing cysteine residues can be affixed to thiol group-modified oligonucleotides via disulfide bond formation. Eventually the release of the oligonucleotide within the cell can be achieved by using a reducing agent to break the disulfide bond.
• Non-Covalent Complexation: Positively charged CPPs can interact with negatively charged oligonucleotides through ion pairing or complexation.

2. Conjugation Purification & Identification: BOC Sciences has optimized purification and evaluation methods to ensure the quality of the conjugates.

3. Detailed Analytical Reports: BOC Sciences will provide you with a detailed conjugate-based identification report at the end to ensure high quality delivery.

Figure 2. Service flow of CPP-oligonucleotide conjugation.

• Why Choose Our CPP-Oligonucleotide Conjugation Services?

Ultra-high purity

BOC Sciences' unique separation, purification and characterisation solutions ensure high purity of conjugation products.

Efficient Delivery Times

BOC Sciences' large-scale synthesis capabilities are recognised by many customers, ensuring on-time or even early delivery.

Technical Support from Experienced Experts

With decades of experience in the synthesis of oligonucleotides, BOC Sciences' experts provide expert technical support and advice.

On-demand Customisation Available

Based on a proprietary oligonucleotide coupling customisation solution, BOC Sciences offers a flexible on-demand customisation service.

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Cell-Penetrating Peptide-Oligonucleotide Conjugation

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