DNP Labeling of Oligonucleotides

DNP (2,4-dinitrophenyl) labeling can be achieved by covalent binding to specific functional groups in the molecule. BOC Sciences offers labeling oligonucleotides services for a wide range of molecule types, which are coupled to each other via amino, carboxyl, and sulfhydryl groups to form labeled oligonucleotide compounds for a wide range of application scenarios.

Why choose DNP Labeled Oligonucleotides?

DNP, as a chemical labeling reagent, is highly electrophilic and can provide several advantages when labeling oligonucleotides.

• High sensitivity - DNP labeling is highly sensitive and provides a strong detection signal.
• Stability - DNP labeling has a high stability of the reaction product and can maintain labeling stability under a variety of experimental conditions.
• Versatility - DNP labeling can be applied to a wide range of biomolecules, including oligonucleotides, proteins and peptides.
• Traceability - Because DNP labeling has a specific chemical structure, it can be traced and detected using DNP-specific antibodies or other DNP probes.
• Compatibility - DNP labeling can be compatible with other labeling methods and detection systems, such as fluorescent labeling, enzyme labeling, and so on.

DNP Labeling Oligonucleotide Service

• DNP labeling pre-treatment service

In order to make the labeled oligonucleotides have rich and diversified functions, DNP can be pre-treated before labeling. Pre-treatment includes DNP activation and modification, and DNP activation can be carried out by choosing suitable activators to make it better bind to oligonucleotides. And DNP modification can usually be used as follows.

• Amino modification - If the molecule to be labeled contains a free amino (-NH2) functional group, the DNP activated ester can be used to react with the amino group. Under basic conditions, the DNP-activated ester will form an amide bond with the amino group, thus introducing DNP into the molecule.
• Hydroxyl modification - For compounds containing a free hydroxyl group (-OH), DNP-activated esters or other reactive DNP reagents can be used to esterify with the hydroxyl group.
• Sulfhydryl modification - For molecules containing sulfhydryl groups (-SH), the sulfhydryl group can be reacted with the sulfhydryl group using DNP-activated esters or other reactive DNP reagents.

• Custom synthesis of DNP-labeled oligonucleotides

Based on the pretreatment of DNP molecules and our customizable oligonucleotides, BOC Sciences offers custom synthesis of DNP-labeled oligonucleotides. During the preparation process, the chemical properties of DNP reagents and oligonucleotides need to be considered. Typically, the DNP labeling reaction is carried out under alkaline conditions to promote the reaction of the DNP reagent with specific functional groups in the oligonucleotide.

• Quality monitoring of DNP-labeled oligonucleotides

When quality monitoring is performed, comparisons should be made with unlabeled oligonucleotides and it should be ensured that appropriate control samples are used. This allows the presence, purity, and quality of the label to be determined and the effectiveness and stability of the label to be assessed. BOC Sciences can typically perform quality monitoring of DNP-labeled oligonucleotides using UV-Vis absorption spectroscopy, gel electrophoresis techniques, fluorescence measurements, etc. BOC Sciences has the capability to perform quality monitoring of DNP-labeled oligonucleotides using a wide range of technologies.

BOC Sciences has advanced oligonucleotide separation and purification systems, testing equipment, many years of experience in oligonucleotide synthesis or chemical synthesis, and an advanced service system to provide high-quality services to research users around the world. In addition, we also provide scale-up production to meet customer needs at competitive prices. If you are interested in our services, please feel free to contact us.

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DNP Labeling of Oligonucleotides

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