Transfer RNA-derived small RNAs (tsRNAs) have emerged as crucial regulators in gene expression, stress response, and intercellular communication. These small RNA fragments, generated through specific cleavage of mature or precursor tRNAs, exhibit diverse biological activities—from modulating translation and ribosome biogenesis to influencing epigenetic control and disease development. The increasing recognition of tsRNAs as key molecular players in cancer, neurodegenerative disorders, and metabolic diseases has created an urgent demand for high-quality synthetic tsRNAs that can be precisely designed, chemically modified, and functionally validated. At BOC Sciences, we offer comprehensive tsRNA Synthesis Services to meet the most demanding research needs. Our expertise in oligo synthesis and RNA chemistry enables us to deliver tailor-made tsRNAs with exceptional sequence accuracy, structural integrity, and modification flexibility—empowering researchers to confidently explore tsRNA function and mechanism in diverse biological systems.
Research on transfer RNA-derived small RNAs (tsRNAs) often faces technical barriers that limit data quality and reproducibility. BOC Sciences' tsRNA Synthesis Service is designed to overcome these challenges by delivering highly precise, modification-accurate, and reproducible synthetic tsRNAs.
Reliable Reproducibility: Consistent synthesis protocols eliminate batch-to-batch variation and ensure dependable experimental outcomes.
Authentic Structural and Chemical Fidelity: Incorporation of natural modifications (e.g., m1A, m5C, Ψ) ensures that synthetic tsRNAs accurately mimic native molecules.
Enhanced RNA Stability and Function: Chemically stabilized tsRNAs maintain integrity during transfection, hybridization, and in vitro assays.
High Purity and Precision: Advanced HPLC purification and LC-MS validation remove truncated or impure fragments that could distort data.
Custom Flexibility: Tailored synthesis options—including length, modification, labeling, and scale—meet diverse project and assay requirements.
By resolving these key issues, BOC Sciences provides researchers with reliable, high-quality tsRNAs that support accurate functional studies, biomarker discovery, and mechanistic exploration with full experimental confidence.
At BOC Sciences, our tsRNA Synthesis Service is not a general oligonucleotide production platform — it is a dedicated, application-driven synthesis system developed to meet the distinct molecular and biochemical requirements of transfer RNA-derived small RNAs (tsRNAs). We offer precisely engineered tsRNA molecules that accurately reflect endogenous variants in sequence, length, and modification profile. Each tsRNA we synthesize is tailored to support high-level research on tsRNA-mediated regulation, biomarker validation, and RNA-protein interactions.
BOC Sciences offers comprehensive synthesis of all major tsRNA categories, providing researchers with the versatility to explore the full regulatory spectrum of tRNA-derived fragments:
| tsRNA Categories | Description | Price |
| tRF-5 Series | Generated from the 5′ end of mature tRNAs (typically 14–30 nt). These fragments are often involved in translational repression, stress adaptation, and intercellular signaling. | Inquiry |
| tRF-3 Series | Originating from the 3′ end and frequently containing a CCA tail, these tsRNAs are widely used in microRNA-like studies due to their participation in Argonaute-mediated gene silencing. | Inquiry |
| tRF-1 Series | Derived from pre-tRNA trailers, tRF-1 fragments are increasingly recognized for their roles in cell differentiation and proliferation regulation. | Inquiry |
| tiRNAs (tRNA Halves) | Longer fragments (30–35 nt) formed by angiogenin-mediated cleavage under stress; key regulators of global translation shutdown and stress granule formation. | Inquiry |
| Chemically Stabilized tsRNA Mimics | Engineered for gain-of-function studies, these tsRNAs incorporate stabilizing modifications such as 2′-O-methyl or phosphorothioate linkages, extending half-life and preserving native secondary structures—ideal for cell-based or translation assays. | Inquiry |
| tsRNA Antagonists/Antagomirs | Designed for loss-of-function experiments, these antisense inhibitors bind specifically to endogenous tsRNAs, blocking target interactions and enabling pathway-level functional dissection. | Inquiry |
| Reporter-Linked tsRNAs | Conjugation with fluorescent or luciferase tags enables visualization of intracellular localization, processing dynamics, and turnover in live-cell systems. | Inquiry |
| Custom or Chimeric tsRNAs | Specially designed hybrids or artificial constructs that mimic cleavage variants or engineered fragments for structure–function investigation. | Inquiry |
Whether for mechanistic analysis or assay standardization, each tsRNA type can be synthesized in both naturally modified and synthetic unmodified forms to meet specific experimental goals.
BOC Sciences provides end-to-end custom sequence design and optimization to ensure each tsRNA exhibits both chemical fidelity and biological relevance. Our team collaborates closely with researchers to reconstruct sequences that reflect authentic cleavage patterns and conformational stability.
Through this integrative approach, BOC Sciences delivers synthetic tsRNAs that not only match the native sequence but also retain the correct biochemical behavior in experimental systems.
Because tsRNAs are defined by their rich modification landscape, accurate functional reproduction requires precise incorporation of post-transcriptional marks. BOC Sciences provides one of the most extensive tsRNA modification portfolios available, applying state-of-the-art phosphoramidite chemistry to ensure authentic molecular mimicry. Supported modifications include:
Clients may request fully modified tsRNAs that precisely reproduce endogenous species, partially modified analogs to probe biological relevance, or synthetic controls for comparative mechanistic studies.
Precise control over fragment length and terminal composition is fundamental for maintaining the structural authenticity and biological relevance of tsRNAs. Even subtle variations in nucleotide length or terminal chemistry can significantly alter tsRNA function, target affinity, and cellular stability. To ensure complete experimental flexibility, BOC Sciences offers fine-tuned customization of both tsRNA fragment size and end configuration to match natural cleavage or designed functional requirements.
Our synthesis system supports a full range of fragment sizes:
In addition, we enable precise end-group control to reflect natural enzymatic cleavage signatures or experimental designs:
By reproducing both the sequence-specific architecture and terminal chemistry of native tsRNAs, BOC Sciences ensures that each synthetic molecule behaves predictably in downstream assays—whether for mechanistic research, binding assays, or cellular functional validation.
BOC Sciences employs a rigorously optimized tsRNA synthesis workflow that integrates advanced chemical techniques with stringent analytical validation. Each step is engineered for precision, reproducibility, and authenticity, ensuring that every tsRNA faithfully mimics its natural counterpart.
Each project begins with an in-depth consultation to understand the biological objectives, target tsRNA type (5′ or 3′ fragment), and desired modifications. Our scientific team uses advanced design algorithms and tRNA cleavage mapping data to develop optimized synthetic blueprints that mimic endogenous tsRNAs.
Before synthesis begins, each tsRNA sequence undergoes predictive modeling to assess folding stability, base pairing, and modification feasibility. This proprietary computational screening minimizes synthesis errors and ensures optimal yield for complex or heavily modified sequences.
Using next-generation phosphoramidite chemistry optimized for short RNA fragments, tsRNAs are synthesized on automated high-precision platforms. Reaction conditions are fine-tuned in real-time to maintain coupling efficiency, enabling accurate incorporation of natural and modified nucleotides.
After synthesis, the tsRNA is carefully deprotected and cleaved from the solid support. BOC Sciences employs multi-dimensional HPLC and PAGE purification to remove truncated sequences, side products, and residual reagents, resulting in a high product purity.
Every synthesized tsRNA undergoes rigorous multi-parameter characterization, including LC-MS for mass validation, CE or PAGE for size verification, and UV spectroscopy for concentration and purity assessment. This ensures structural integrity, modification accuracy, and experimental consistency.
The finalized tsRNA is delivered lyophilized with complete QC documentation and analytical data. Our team provides continued technical support—offering guidance on resuspension, storage, and downstream applications to ensure immediate usability and reliable experimental outcomes.
At BOC Sciences, we combine scientific rigor with advanced technology to deliver tsRNA molecules that meet the highest research standards. Our expertise in RNA chemistry allows us to synthesize even the most complex tsRNA sequences with exceptional accuracy and purity.
Expertise in Small RNA Chemistry: Our team of seasoned scientists possesses extensive experience in the synthesis of small non-coding RNAs, ensuring each tsRNA reflects natural sequence fidelity and functionality.
Comprehensive Modification Capabilities: We provide a broad spectrum of chemical and structural modifications—methylations, pseudouridylation, 2′-O-methylation, and more—to faithfully mimic endogenous tsRNAs.
High Purity and Analytical Assurance: Every batch is rigorously purified (HPLC/PAGE) and verified by LC-MS and UV spectroscopy to ensure exceptional quality and reproducibility.
Flexible Customization: From sequence design to labeling and scale-up production, all synthesis parameters are tailored to meet your specific research needs.
Dedicated Technical Support: Our project managers and RNA experts guide clients through design, synthesis, and validation, offering professional insight at every step.
Global Trust and Reliability: Researchers worldwide rely on BOC Sciences for consistent, high-performance oligonucleotide products that accelerate scientific discovery.
With BOC Sciences, you gain not only a synthesis provider but a committed scientific partner devoted to advancing your tsRNA research with accuracy, reliability, and innovation.
The ability to access synthetically engineered tsRNAs—precisely mimicking endogenous tRNA-derived small RNAs—opens up a unique experimental landscape for decoding their diverse biological roles. Synthetic tsRNAs are not simply substitutes for natural molecules; they are indispensable analytical standards and experimental tools that allow researchers to dissect sequence-dependent effects, structural modifications, and cellular mechanisms with unprecedented control and reproducibility.
Synthetic tsRNAs serve as powerful molecular probes to decipher their regulatory mechanisms in gene expression and translation control. Researchers use custom tsRNAs to:
Through the availability of structurally verified synthetic tsRNAs, mechanistic insights can be achieved without confounding variables present in cell-extracted RNA samples.
Because tsRNAs carry a high density of post-transcriptional modifications, they are valuable tools for epitranscriptomic profiling. Chemically defined synthetic tsRNAs enable:
BOC Sciences' modification-accurate synthesis allows researchers to replicate native tsRNA chemistry—something not possible with standard siRNA or miRNA synthesis methods.
Quantitative and reproducible detection of tsRNAs in biological samples relies on synthetic reference standards. Our high-purity tsRNAs support:
In biomarker discovery pipelines, synthetic tsRNAs from BOC Sciences ensure consistent measurement accuracy across platforms and laboratories.
Custom tsRNAs enable systematic screening of their regulatory potential on cellular pathways, including:
By providing site-specifically modified and structurally stable tsRNAs, BOC Sciences empowers clients to move from exploratory studies to target validation and lead discovery more efficiently.
Labeled tsRNAs synthesized with biotin or fluorescent tags are essential for characterizing tsRNA–protein interactomes. These molecules can be used to:
Such approaches are transforming our understanding of tsRNAs as active participants in post-transcriptional regulation rather than passive by-products of tRNA cleavage.
BOC Sciences can produce tsRNA variant libraries to enable large-scale functional screens that assess:
These systematic assays provide a foundation for identifying therapeutically relevant tsRNAs and for mapping tsRNA regulatory networks at genome-wide resolution.
Custom tsRNA synthesis transforms the way scientists interrogate this emerging RNA class. Whether used for functional dissection, biomarker validation, or epitranscriptomic calibration, synthetic tsRNAs from BOC Sciences offer the purity, customization, and modification precision required for credible, reproducible, and mechanistically insightful research outcomes.
tsRNA synthesis refers to the artificial production of transfer RNA-derived small RNAs (tsRNAs), which are small fragments generated from tRNA cleavage. Synthetic tsRNAs enable researchers to study their biological roles, validate biomarkers, and explore therapeutic targets in controlled experimental settings.
Most tsRNAs range from 15 to 35 nucleotides, depending on their origin (5′-derived or 3′-derived fragments). Our synthesis platform supports custom lengths beyond this range if required for mechanistic studies, enabling researchers to test truncated or extended variants for structure–function analysis.
Yes. Our synthesis technology enables site-specific incorporation of natural modifications such as N¹-methyladenosine (m1A), 5-methylcytidine (m5C), pseudouridine (Ψ), 2′-O-methylation, and thiolation. These modifications are introduced using pre-modified phosphoramidite monomers or selective post-synthetic derivatization, allowing faithful recreation of endogenous tsRNA structures and improved biological stability.
Each synthesized tsRNA undergoes mass spectrometric and chromatographic characterization. LC-MS confirms molecular weight and verifies that all modifications are correctly incorporated, while HPLC and PAGE analyses assess purity and identify potential truncated products. For modified tsRNAs, additional UV or CE profiling is performed to validate base modification patterns.
Lyophilized tsRNAs should be stored at −20°C or below in RNase-free tubes. Upon reconstitution in RNase-free water or TE buffer (pH 7.5–8.0), samples should be aliquoted to avoid repeated freeze–thaw cycles. For long-term stability, storing in buffers containing RNase inhibitors or low ionic strength is recommended.
Yes. We provide incorporation of modified backbones (e.g., phosphorothioate, boranophosphate) and base analogs (e.g., 2-aminopurine, 5-bromouracil). These analogs are valuable tools for structural probing, fluorescence-based studies, and investigations into RNA–protein recognition dynamics.
Our service covers a broad range—from nanomole quantities for discovery research to gram-scale production for preclinical testing or screening campaigns. Process scalability is fully validated, maintaining identical synthesis conditions to ensure uniformity between small- and large-scale batches.
Partner with BOC Sciences today to receive expert consultation and a customized quote for your tsRNA synthesis project.
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