In vitro optimization and in vivo regulation of the properties of siRNAs are often inseparable from chemical modifications. Chemically modified siRNAs exhibit better stability, specificity and cellular uptake, and furthermore will yield better gene silencing effects. BOC Sciences is dedicated to provide you with professional base-modified siRNA, and also includes custom modified siRNA on demand.
Modified bases, also known as rare bases, are derivatives of bases other than the major base (ATCGU). tRNAs tend to contain a high number of rare bases, with some tRNAs containing up to 10% rare bases. Similar to the functions of other chemical modifications, base modifications have an important role in tuning and optimizing siRNA properties, including stability, nuclease resistance, cellular uptake, targeting, cytotoxicity, immunogenicity and gene silencing efficiency. For example, pseudouridine modification is a hot and very critical step in RNA vaccine development today, and is important in determining the efficiency of vaccine action.
Figure 1. Base modifications of siRNA.
More new ideas for base modifications are also being developed to meet various scientific research needs, and the diversity of nucleic acid molecule structures and functions meet this need exactly. Customers have full access to custom properties and functions of siRNAs at BOC Sciences based on the different modifications explored by our expert team.
| Modification | Short Code | Purification | Details | Price |
| 1-Methyl-guanosine | m1G | HPLC | 1-methyl-guanosine is guanosine substituted with a methyl group at position N-1. | Inquiry |
| 2,6-Diaminopurine | DAP | HPLC | It is a purine nucleobase, structurally similar to adenine, but with two amino groups (-NH2). | Inquiry |
| 2-Methyl-adenosine | m2A | HPLC | A modified nucleoside derived from adenosine with the methyl(-CH3) attached to the nitrogen atom at the 2-position of the adenine base. | Inquiry |
| 2-Aminopurine | 2AP | HPLC | Modified base derivative derived from adenine, which has an amino group (-NH2) located at the 2-position of the purine ring, replacing the ketone group in adenine. | Inquiry |
| 4-Thio-uridine | 4-S-U | HPLC | A modified nucleoside obtained from uridine in which the sulfur atom (-S) replaces the oxygen atom at the 4-position of the uracil base. | Inquiry |
| 5-Bromo-Uridine | U[5Br] | HPLC | 5-Bromouridine is a modified nucleoside of uridine in which a bromine atom (-Br) replaces the hydrogen atom at the 5-position of the uracil base. | Inquiry |
| 5-Fluoro-cytidine | C[5F] | HPLC | A fluorine atom (-F) is substituted for the hydrogen atom at the 5-position of the cytidine base. | Inquiry |
| 5-Fluoro-uridine | U[5F] | HPLC | A fluorine atom (-F) is substituted for the hydrogen atom at the 5-position of the uracil base. | Inquiry |
| 5-Iodo-uridine | U[5I] | HPLC | An iodine atom (-I) is substituted for the hydrogen atom at position 5 of the uracil base. | Inquiry |
| 5-Methyl-cytidine | 5-M-C | HPLC | A methyl group (-CH3) replaces the H atom at the 5-position of the cytidine base. | Inquiry |
| 5-Methyl-Deoxycytidine | 5-M-dC | HPLC | A methyl group (-CH3) replaces the H atom at the 5-position of the cytosine base. | Inquiry |
| 5-Methyl-uridine | rT | HPLC | A methyl group (-CH3) is attached to the 5-position of the uracil base. | Inquiry |
| Inosine | I | HPLC | Inosine is a naturally occurring adenine nucleoside that does not require artificial modification. | Inquiry |
| N2-Methyl-guanosine | m2G | HPLC | A methyl group (-CH3) is attached to the nitrogen atom at the 2 position of the guanine base. | Inquiry |
| N3-Methyl-uridine | 3-M-U | HPLC | In N3-methyluridine, a methyl group (-CH3) is affixed to the nitrogen atom at the 3-position of the uracil base. | Inquiry |
| N6,N6-Dimethyl-adenosine | DMA | HPLC | Two methyl groups (-CH3) are attached to the nitrogen atom at the 6 position of the adenine base. | Inquiry |
| N6-Methyl-adenosine | m6A | HPLC | A methyl group (-CH3) is linked to the nitrogen atom at the 6-position of the adenine base. | Inquiry |
| O6-Methyl-guanosine | O6mG | HPLC | Methyl (-CH3) is attached to the oxygen atom at the 6 position of the guanine base. | Inquiry |
| Pseudouridine | Ψ | HPLC | Uridines are isomerized to form pseudouridine. | Inquiry |
| Purine ribonucleoside | Pu | HPLC | A group of nucleosides consisting of a purine base attached to a ribose molecule. | Inquiry |
| Pyrrolo-cytidine | pC | HPLC | Modified RNA base. | Inquiry |
| Ribavirin | RBV | HPLC | Ribavirin is a 1-ribosyltriazole that is the 1-ribofuranosyl derivative of 1,2,4-triazole-3-carboxamide. | Inquiry |
BOC Sciences has a team of experts with a decade of experience in RNA synthesis and advanced equipment to ensure high quality and competitive price for base modified siRNA. For more custom base modified siRNA, please feel free to contact us.
Base modifications enhance siRNA stability against nucleases, improve target specificity, reduce off-target effects, and optimize thermal properties while maintaining high gene silencing efficiency.
We recommend modifications including 5-methylcytidine, pseudouridine, 2-aminopurine, and inosine, which have demonstrated significant improvements in siRNA performance and stability.
Yes, we specialize in creating hybrid modification strategies that integrate base modifications with ribose and backbone modifications for optimal siRNA performance.
Strategic base modifications enhance binding specificity to target sequences, reduce mismatched hybridization, and significantly decrease off-target effects through improved molecular recognition.
All base-modified siRNAs undergo comprehensive analysis including HPLC purity verification, mass spectrometry confirmation, and functional validation in gene silencing assays.
Our team can develop specialized base modifications tailored to unique experimental requirements, with consultation available for novel modification strategies and applications.
