BOC RNA can customize a full range of high-quality oligonucleotide modification and obtain artificial modifications including backbones, bases, sugars, and internucleotide bonds oligonucleotides.
Using our synthesis platform can reduce experiment costs for our customers by improving coupling efficiency. We can meet the needs of various synthesis scales, purification options, and modifications and allow large-scale production in a short time.
Modified oligonucleotides not only can enhance affinity but also increase the resistance to endonuclease and exonuclease, thereby having high stability in vitro and in vivo.
|Modifications||Nuclease Resistance||RNase H Activation|
|Bridged Nucleic Acids (BNA)|
|2' Fluoro RNA|
|2' O-Methyl RNA (2'OMe)|
We have many useful modified base analogs to be incorporated during oligonucleotide synthesis. The use of modified bases can increase the melting temperature, thereby improving specificity and sensitivity, and is suitable for detecting small or highly similar DNA or RNA targets.
Base modifications are divided into the following categories:
Oligonucleotides containing reverse base RNA or DNA linkages can be used for antisense research or for synthesizing oligonucleotide fragments with the opposite meaning to normal synthesis for structural research. BOC RNA synthesizes base inversion oligonucleotides, which can be used to produce oligonucleotides with 5'-5' or 3'-3' linkages or to combine them in the same oligonucleotide.
It is recommended to purify anti-base modified oligonucleotides. The quality of each synthesized oligonucleotide can be strictly controlled through mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis.
According to the position where the modification is introduced, the linker and the chemical modification of the oligonucleotide can be divided into three categories
BOC RNA provides a variety of spacer modified oligonucleotides. These spacers have different atomic numbers. They are usually used between the functional part of the oligonucleotide and the hybridization region as the steric hindrance.
|C3 Spacer||SpC3||Introduce linker arm, prevent enzymatic degradation, a DNA abasic site, an effective chain terminator|
|C6 Spacer||SpC6||Introduce linker arm|
|C12 Spacer||SpC12||Introduce linker arm|
|Spacer 9||Sp9||Introduce linker arm, hydrophilic spacer|
|Spacer 18 (hexaethyleneglycol)||Sp18||Introduce linker arm, hydrophilic spacer|
|dSpacer (Abasic furan)||dSp||DNA abasic site, prevent enzymatic degradation|
|ribospacer rSpacer||rSp||RNA abasic site, prevent enzymatic degradation|
|Photocleavable PC Spacer||PLC||photocleavable spacer|
Fluorescence labeled oligonucleotides can be used as hybridization probes for various in vivo and in vitro research/diagnostic applications, as well as structural and functional studies of DNA, RNA, and protein-oligonucleotide complexes. Contact BOC RNA for a spacer fluorescently labeled oligonucleotide synthesis service.
|6-FAM (NHS ester)||496/516||Yellow-green|
|JOE (NHS ester)||529/555||Yellow|
|TAMRA (NHS ester)||559/583||Yellow-orange|
|MAX (NHS ester)||531/556||Yellow|
|ROX (NHS ester)||588/608||Orange|
3'and 5'-phosphorylated oligonucleotides can be used for further chemical modification or as substrates in DNA repair research. 5'phosphorylated oligonucleotides can be directly connected to the vector without using T4 polynucleotide kinase in advance. 3'phosphorylation can inhibit the degradation of some 3'-exonucleases. In addition, chemical phosphorylation allows a large number of labeling and is more reproducible than enzymatic methods.
BOC RNA provides phosphorylated oligonucleotide synthesis of various scales and purity to meet different needs.
Contact us now to get technical advice and more information. Our technical support team will respond to your questions or provide a quotation as soon as possible.