Oligo Modifications

BOC RNA can customize a full range of high-quality oligonucleotide modifications and obtain artificial modifications including backbones, bases, sugars, and internucleotide bonds oligonucleotides.

Using our synthesis platform can reduce experiment costs for our customers by improving coupling efficiency. We can meet the needs of various synthesis scales, purification options, and modifications and allow large-scale production in a short time.

BOC Sciences' Oligonucleotide Modifications

• Oligo fluorescent labeling

Fluorescent labeling is by far the most commonly used labeling for oligonucleotide synthesis, and it is possible to transduce analyte-bioreceptor binding into analytical signals by attaching one or more fluorophore groups or fluorescent compounds to oligonucleotide chains. Oligo fluorescent labeling is extensively used in gene sequencing, forensic and genetic analysis.

• Oligo backbone modification

The oligonucleotide backbone consists of phosphodiester linkages and sugar moieties, which can be properly modified to improve the properties of various oligonucleotides, including improved stability to nuclease and uptake into cells, increased affinity for complementary strands, enhanced kinetics and base pairing specificity and sensitivity to binding to nucleic acid targets.

• Oligo base modification

Base modifications can alter the quality and affect the structure of oligonucleotides, sometimes changing UV absorbance, molecular weight and melting temperature (T_m), base mismatch detection and oligonucleotide repair. Diverse base modifications provide many improvements for efficient, large-scale genome engineering and the application of nucleic acid therapeutics, such as antisense oligonucleotides (ASO), and small interfering RNA (siRNA).

• Oligo splicing and chemical modification

Splicing refers to the editing of newly transcribed pre-messenger RNA (pre-mRNA) by removing introns (non-coding regions of the gene) and adding or joining exons (coding regions). Mature mRNA is thus created, which is then used as a template for the synthesis of specific proteins.

• Oligo spacer modification

During oligonucleotide synthesis, spacer arms are introduced into the sequence using spacer phosphoramidites. Multiple additions of different spacer molecules allow control of the exact length of the spacer arm.

• Oligo phosphorylation modification

Phosphorylation, a vital modification in biochemistry and molecular biology, is a chemical reaction that adds a phosphate group (PO^3-) to an organic molecule. Oligonucleotide phosphorylation plays an important role in oligonucleotide modification, facilitating researchers to further investigate the structure and function of DNA and RNA oligonucleotides and apply nucleic acid molecules in the fields of medicine and genetic engineering.