Oligo Modifications

BOC RNA can customize a full range of high-quality oligonucleotide modifications and obtain artificial modifications including backbones, bases, sugars, and internucleotide bonds oligonucleotides.

Using our synthesis platform can reduce experiment costs for our customers by improving coupling efficiency. We can meet the needs of various synthesis scales, purification options, and modifications and allow large-scale production in a short time.

Modification Options

Fluorescent labeling is by far the most commonly used labeling for oligonucleotide synthesis, and it is possible to transduce analyte-bioreceptor binding into analytical signals by attaching one or more fluorophore groups or fluorescent compounds to oligonucleotide chains. Oligo fluorescent labeling is extensively used in gene sequencing, forensic and genetic analysis.

ClassificationModificationsPurification
Fluorescent labeling6-FAM (NHS ester)HPLC/PAGE
6-FAM (Fluorescein)
Fluorescein dT
Cy3
Cy3.5
TAMRA
JOE (NHS ester)
Cy5
TAMRA (NHS ester)
MAX (NHS ester)
TET
Cy5.5
ROX (NHS ester)
TYE 563
HEX
TEX 615
TYE 665
TYE 705
SUN

The oligonucleotide backbone consists of phosphodiester linkages and sugar moieties, which can be properly modified to improve the properties of various oligonucleotides, including improved stability to nuclease and uptake into cells, increased affinity for complementary strands, enhanced kinetics and base pairing specificity and sensitivity to binding to nucleic acid targets.

ClassificationModificationsPurification
Backbone modificationBridged Nucleic Acids (BNA)HPLC/PAGE
2' Fluoro RNA
2' O-Methyl RNA (2'OMe)
2'-F-ANA
L-DNA
L-RNA
Phosphorothioate DNA
Phosphorothioate RNA
Phosphonoacetate (PACE)
Methylphosphonate linkages
ZNA Spermine

Base modifications can alter the quality and affect the structure of oligonucleotides, sometimes changing UV absorbance, molecular weight and melting temperature (Tm), base mismatch detection and oligonucleotide repair. Diverse base modifications provide many improvements for efficient, large-scale genome engineering and the application of nucleic acid therapeutics, such as antisense oligonucleotides (ASO), and small interfering RNA (siRNA).

ClassificationModificationsPurification
Base modification2,6-Diaminopurine-2'-deoxyriboside (DAPdR)HPLC/PAGE
2-Amino Purine deoxyribose
2-Amino Purine ribose
4-Thio-Uridine
5-bromo deoxycytosine (Br dC)
EDTA 2'- deoxythymidine
N3-Methyl deoxy Cytidine
7-deaza-2'-deoxyguanosine
8-Oxo-Guanosine

Splicing refers to the editing of newly transcribed pre-messenger RNA (pre-mRNA) by removing introns (non-coding regions of the gene) and adding or joining exons (coding regions). Mature mRNA is thus created, which is then used as a template for the synthesis of specific proteins.

ClassificationModificationsPurification
Splicing and chemical modificationPMOHPLC/PAGE
2'-OMePS

During oligonucleotide synthesis, spacer arms are introduced into the sequence using spacer phosphoramidites. Multiple additions of different spacer molecules allow control of the exact length of the spacer arm.

ClassificationModificationsPurification
SpacerSpC3HPLC/PAGE
SpC6
SpC12
Sp9
Sp18
dSp
rSp
PLC

Phosphorylation, a vital modification in biochemistry and molecular biology, is a chemical reaction that adds a phosphate group (PO3-) to an organic molecule. Oligonucleotide phosphorylation plays an important role in oligonucleotide modification, facilitating researchers to further investigate the structure and function of DNA and RNA oligonucleotides and apply nucleic acid molecules in the fields of medicine and genetic engineering.

ClassificationModificationsPurification
Phosphorylation3-PhosHPLC/PAGE
5'-P
Tri-Phos

Applications

  • Modified oligonucleotides help to understand the mechanism of many biochemical reactions and processes.
  • Modified oligonucleotides have been used as probes in various diagnostic and therapeutic applications.
  • Used in drug discovery and disease treatment (nucleic acid vaccines and nucleic acid drugs) research.

Services Process

Oligo Modifications

Our Ability

  • Flexible production scale: 20 nmol, 50 nmol, 100 nmol, 200 nmol, 1 µmol, 5 µmol, and 10 µmol. Bulk orders can be provided upon request.
  • Purity: Desalted, PAGE, HPLC, dual HPLC purification process is adopted, which is 3%~5% higher than the industry average.
  • Physical isolation of synthesis, ammonolysis, purification, and concentration to prevent cross-contamination.
  • Complete variety: BOC RNA provides comprehensive modified oligonucleotide synthesis services. You might be interested in our DNA/RNA modifications services.

Contact us now to get technical advice and more information. Our technical support team will respond to your questions or provide a quotation as soon as possible.

* Only for research. Not suitable for any diagnostic or therapeutic use.
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