RNA, as an important research tool, is widely used in the analysis of gene function and the development of novel therapeutic strategies. BOC RNA has advanced international high-throughput DNA synthesizers, professional technicians and mature synthetic purification methods to provide you with high-quality, multi-kind RNA synthesis services in a timely manner.
RNA modification refers to covalent modification on RNA, such as m6A (methylation of position 6 N on adenine). In addition, various complex covalent modifications on tRNA, or cap sequence m7G on mRNA (methylation of position 7 N on guanine) are also RNA modifications. RNA modification can regulate RNA translation and change RNA stability.
2′-O-methylation (2′-O-M) is a chemical modification of ribose RNA methylation at the 2' position under the action of RNA methylase or fibrinase. 2′-O-methylation modifications are common in rRNAs and snRNAs associated with translational and RNA shear functions, and also in mRNA, tRNA, snoRNA and siRNA, and in the four bases of A, U, C and G in both three-domain organisms and viral RNA. Studies have shown that 2′-O-methylation affects mRNA binding to protein, regulates rRNA translation efficiency and participates in tRNA recognition. More than 1200 2'-O-methylation sites have been identified in mammals and yeast.
Fig. 1 2'-O-2'-O-methylated adenosine (2'-O-MA, Am)
2'-Omethyl bases are classified as 2'-Omethyl RNA monomers. When no specific 2'-OH is required, 2'-Omethyl nucleotides are most commonly used to give nuclease resistance to oligonucleic acids designed for antisense, siRNA, or aptamer-based research, diagnosis, or treatment purposes. Nuclease resistance can be further enhanced by phosphothiolation of appropriate inter-nucleotide linkages within the oligonucleotide.
2'-Omethyl modification is a form of natural modification that occurs after transcription of small RNA (e.g. tRNA), which increases RNA: RNA double-stranded Tm, but causes only minor changes in RNA: DNA hybrid strand stability. It is typically 5 to 10 times less sensitive to DNA enzymes than DNA and can be partially resistant to a variety of ribonucleases and deoxyribonucleases. It is commonly used in antisense experiments to increase stability as well as binding affinity to the target.
| 2'-Omethyl RNA Bases | Short Code | Price |
| 2'-OMe RNA Bases (A) | mA | Inquiry |
| 2'-OMe RNA Bases (C) | mC | Inquiry |
| 2'-OMe RNA Bases (G) | mG | Inquiry |
| 2'-OMe RNA Bases (U) | mU | Inquiry |
The expert team at BOC RNA has extensive experience in the field of custom oligonucleotide synthesis and modification. We are committed to providing end-to-end customized DNA/RNA modification services ranging from consulting, DNA/RNA oligonucleotide design, production and purification, to quality control and delivery. Please contact us for more details.
RNA modification involves covalent changes to RNA molecules, such as methylation, which can regulate RNA translation and stability. These modifications are critical for understanding gene expression and RNA function in various biological processes.
2'-O-methylation is a modification where a methyl group is added to the 2'position of the ribose sugar in RNA. This modification enhances RNA stability, resistance to nucleases, and improves binding affinity, especially in applications like antisense and RNA interference (RNAi).
2'-O-methylation provides nuclease resistance, making RNA molecules more stable in biological environments. This modification is particularly useful in RNA-based research, such as antisense oligonucleotides and siRNA, by preventing degradation.
2'-O-methyl RNA bases are widely used in antisense, RNAi, aptamer research, and CRISPR technology. They help enhance the stability, specificity, and efficiency of RNA-based experiments and therapeutic approaches.
2'-O-methylation improves RNA stability and binding efficiency, which is beneficial for RNA-based therapies such as gene silencing and CRISPR-based gene editing. It also increases the resistance of RNA to degradation, enhancing its therapeutic potential.
2'-O-methylation increases RNA:RNA duplex stability by raising the melting temperature (Tm), while having only minor effects on RNA:DNA hybrid stability. This makes it ideal for applications that require stable RNA duplex formation.
