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Fluorescent Molecule-Oligonucleotide Conjugation

BOC Sciences is always working on providing the highest quality conjugation services to combine oligonucleotides with fluorescent molecules. With comprehensive experience in conjugation technology and state-of-the-art manufacturing facilities, we are well qualified to meet your needs for novel conjugates.

What is Fluorescent Molecule-Oligonucleotide Conjugation?

The conjugation of fluorescent molecules and oligonucleotides allows the visualisation and detection of oligonucleotides by fluorescence microscopy or other fluorescence-based techniques widely applied in basic biological and biomedical research.

Fluorescent molecule-oligonucleotide conjugation.Figure 1. Fluorescent molecule-oligonucleotide conjugation.

The process of conjugation usually consists of two main steps, including the labelling of the oligonucleotide with the reactive group and the attachment of the fluorescent molecule to the reactive group.

Introduction of Fluorescent Molecule-Oligonucleotide Conjugation Services

ServiceFluorescent Molecule-Oligonucleotide Conjugation
Customer providesOligonucleotide sequence
PurificationQC analysis by MS, HPLC, or PAGE
DeliveryTypical delivery is lyophilized samples in individual vials that are fully labeled
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1.Design & synthesis of oligonucleotide sequences to meet customer requirements.

2.Selection & preparation of fluorescent molecules to meet customer requirements.

  • Fluorescein
  • BODIPY dyes
  • Rhodamine
  • Oregon Green
  • Cyanine dyes
  • Alexa Fluor 488
  • Alexa Fluor dyes
  • DAPI
  • Texas Red
  • SYBR Green
  • Atto dyes
  • Cascade Blue

3. Covalent binding of modified oligonucleotides to fluorescent molecules.

4. Purification and characterisation of products.

5. Delivery of high quality products and detailed analytical reports.

Service flow of fluorescent molecule-oligonucleotide conjugation.- BOC SciencesFigure 2. Service flow of fluorescent molecule-oligonucleotide conjugation.

* Only for research. Not suitable for any diagnostic or therapeutic use.
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