BOC Sciences is always working on providing the highest quality conjugation services to combine oligonucleotides with fluorescent molecules. With comprehensive experience in conjugation technology and state-of-the-art manufacturing facilities, we are well qualified to meet your needs for novel conjugates.
What is Fluorescent Molecule-Oligonucleotide Conjugation?
The conjugation of fluorescent molecules and oligonucleotides allows the visualisation and detection of oligonucleotides by fluorescence microscopy or other fluorescence-based techniques widely applied in basic biological and biomedical research.
Figure 1. Fluorescent molecule-oligonucleotide conjugation.
Synthesis Steps for Fluorescent Molecule-Oligonucleotide Conjugation
The process of conjugation usually consists of two main steps, including the labelling of the oligonucleotide with the reactive group and the attachment of the fluorescent molecule to the reactive group.
- Firstly, the oligonucleotide is modified to introduce the reactive group, which can be achieved by adding the modified nucleotide during oligonucleotide synthesis or by post-synthetic modification. Common reactive groups for conjugation include amino groups (-NH2), thiol groups (-SH) or carboxyl groups (-COOH).
- Subsequently, attachment of the fluorescent molecule to the reactive group on the oligonucleotide is achieved by various chemical reactions, such as amidation, thiolation or click chemistry.
Applicationof Fluorescent Molecule-Oligonucleotide Conjugation
- Fluorescence in situ Hybridisation (FISH)
- Real Time PCR Detection
- Fluorescence Imaging
- Microarray Analysis
- Gene Expression Profiling
- DNA Sequencing
- Immunofluorescence Analysis
Introduction of Fluorescent Molecule-Oligonucleotide Conjugation Services
| Service | Fluorescent Molecule-Oligonucleotide Conjugation |
| Customer provides | Oligonucleotide sequence |
| Purification | QC analysis by MS, HPLC, or PAGE |
| Delivery | Typical delivery is lyophilized samples in individual vials that are fully labeled |
| Request a Quote | Inquiry |
What Are Our Fluorescent Molecule-Oligonucleotide ConjugationServices?
1.Design & synthesis of oligonucleotide sequences to meet customer requirements.
2.Selection & preparation of fluorescent molecules to meet customer requirements.
- Fluorescein
- BODIPY dyes
- Rhodamine
- Oregon Green
- Cyanine dyes
- Alexa Fluor 488
- Alexa Fluor dyes
- DAPI
- Texas Red
- SYBR Green
- Atto dyes
- Cascade Blue
3. Covalent binding of modified oligonucleotides to fluorescent molecules.
4. Purification and characterisation of products.
5. Delivery of high quality products and detailed analytical reports.
Figure 2. Service flow of fluorescent molecule-oligonucleotide conjugation.
Why Choose Our Fluorescent Molecule-Oligonucleotide Conjugation Services?
- Extensive Experience & Technology: BOC Sciences has extensive experience and technology in the synthesis, chemical modification, purification and preparation of oligonucleotides.
- Professional Technical Support: BOC Sciences' scientists and technicians have the experience and knowledge to provide our customers with fast, professional and effective technical support.
- Customised Design: BOC Sciences is able to provide a customised design service based on customer needs and experimental objectives to ensure optimal experimental results.
- Ultra-high Purity Products: BOC Sciences uses high quality raw materials and employs optimal reaction conditions and purification procedures to ensure high purity and stability of products.
* Only for research. Not suitable for any diagnostic or therapeutic use.
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