BOC Sciences is always working on providing the highest quality conjugation services to combine oligonucleotides with fluorescent molecules. With comprehensive experience in conjugation technology and state-of-the-art manufacturing facilities, we are well qualified to meet your needs for novel conjugates.
The conjugation of fluorescent molecules and oligonucleotides allows the visualisation and detection of oligonucleotides by fluorescence microscopy or other fluorescence-based techniques widely applied in basic biological and biomedical research.
Figure 1. Fluorescent molecule-oligonucleotide conjugation.
The process of conjugation usually consists of two main steps, including the labelling of the oligonucleotide with the reactive group and the attachment of the fluorescent molecule to the reactive group.
| Service | Fluorescent Molecule-Oligonucleotide Conjugation |
| Customer provides | Oligonucleotide sequence |
| Purification | QC analysis by MS, HPLC, or PAGE |
| Delivery | Typical delivery is lyophilized samples in individual vials that are fully labeled |
| Request a Quote | Inquiry |
1.Design & synthesis of oligonucleotide sequences to meet customer requirements.
2.Selection & preparation of fluorescent molecules to meet customer requirements.
3. Covalent binding of modified oligonucleotides to fluorescent molecules.
4. Purification and characterisation of products.
5. Delivery of high quality products and detailed analytical reports.
Figure 2. Service flow of fluorescent molecule-oligonucleotide conjugation.
Fluorescent molecule-oligonucleotide conjugation involves attaching fluorescent molecules to oligonucleotides to enable visualisation using fluorescence-based techniques. This process is widely used in biological and biomedical research for detecting and imaging nucleic acids.
The process involves modifying the oligonucleotide to introduce a reactive group, followed by the attachment of the fluorescent molecule via chemical reactions such as amidation or thiolation. This results in a stable conjugate that can be used for imaging.
Common fluorescent molecules include fluorescein, rhodamine, BODIPY dyes, Alexa Fluor dyes, DAPI, and SYBR Green, among others. These dyes are selected based on the specific application and desired fluorescence characteristics.
These conjugates are used in a variety of applications such as fluorescence in situ hybridisation (FISH), real-time PCR detection, fluorescence imaging, and gene expression profiling. They are also essential for DNA sequencing and microarray analysis.
