Custom DNA Oligonucleotides Synthesis

Custom DNA Oligonucleotides Synthesis

BOC RNA provides custom DNA synthesis services to help customers save time and money. All customized DNA oligonucleotides are produced under strict quality control standards, and all synthetic fragments are verified to ensure 100% sequence identity.

Introduction of DNA Oligos

  • What are DNA Oligos

DNA oligonucleotides are short fragments of DNA composed of a few nucleotide units. These units are linked together via phosphodiester bonds. Oligonucleotides may range from fewer than 10 nucleotides up to 100 nucleotides in length.

  • Types of DNA Oligos

There are two major types of DNA oligonucleotides: synthetic and natural. Synthetic oligonucleotides are created in the laboratory, while natural oligonucleotides are produced by organisms.

  • Applications of DNA Oligos

DNA oligonucleotides have a broad range of applications in various fields. In the medical field, they are used for gene therapy and diagnostics. In molecular biology, they are used for sequencing, gene synthesis, and genetic engineering. In nanotechnology, DNA oligonucleotides are used as building blocks for creating nano-structures and devices.

  • Synthesis of DNA Oligos

The synthesis of DNA oligonucleotides is a complex process that requires specialized equipment and expertise. It can be achieved by automated synthesis or solid-phase synthesis, both of which utilize chemically modified nucleotides and various reagents.

BOC Sciences' Custom DNA Oligos Synthesis Service

  • Unmodified DNA Oligos Synthesis

We can customize unmodified DNA in multiple scales, from small scale (customized DNA for research) to mass production of GMP DNA oligomers, at very competitive prices. Guaranteed yields for purified, unmodified DNA 20-50 bases in length:

100 nmol250 nmol1 µmol5 µmol10 µmol
PAGE Purification0.5-1 OD2 OD10 OD50 OD100 OD
HPLC Purification1-2 OD4 OD20 OD100 OD200 OD
IE-HPLC Purification1-2 OD4 OD20 OD100 OD200 OD
RNase-Free HPLC Purification1-2 OD4 OD20 OD100 OD200 OD
Dual HPLC Purification0.5-1 OD2 OD10 OD50 OD100 OD
Dual PAGE & HPLC0.5 OD1 OD5 OD25 OD50 OD
Purity and yield may vary due to oligo length, modifications, or sequence composition.
  • DNA Oligos Modification

We also provide thousands of custom DNA modifications, including DNA backbone modifications, modified bases, and fluorescent dye labels. These modified oligonucleotides meet the highest quality specifications for research, diagnostics, OEM, and therapeutic drugs.

Modifications include:

5' modification3' modificationInner modification
5' Phosphorylation3' PhosphorylationdT-Dabcyl
5' Biotin3' BiotindT-Bitoin
5' Digoxigenin3' DigoxigenindT-Digoxigenin
5' Amino linker C63' Amino linker C6dT C6-Aminolinker
5' Amino linker C123' Thiol C6Thiol-C6 S-S
5' Thiol C63' spacer C3Spacer 18
5' FITCA3' FITCPhospothioate
5' FAM3' FAMdU
5' TET3' TETdI
5' JOE3' JOE
5' HEX3' HEX
5' TAMRA3' TAMRA
5' ROX3' ROX
5' Cy-33' DABCYL
5' Cy-53' BHQ1
5' Cy-3.53' BHQ2
5' Thiol-C6 S-S3' Thiol-C6 S-S
5' Fluorescein3' Fluorescein
  • Optional DNA Synthesis Services

  1. Custom oligo synthesis and analytical services
  2. DNA qPCR probes synthesis
  3. Long oligonucleotide synthesis for gene expression
  4. Antisense oligonucleotide synthesis
  5. Large scale oligonucleotide productions
  • Quality Control

  1. Use SEC chromatography to deprotect and desalt DNA oligonucleotides to remove impurities caused by trace molecules.
  2. Quantify olionucleotide twice by ultraviolet spectrophotometry to provide an accurate OD yeid measurement.

To meet different experimental requirements, you can choose from the following different purification methods:

Gel FiltrationReverse-phase Cartridge PurificationHPLC PurificationIEX HPLC PurificationPAGE PurificationDouble Purifications
Gene amplificationGoodGoodGoodGoodGoodExcellent
Sequencing primersNot recommendedGoodGoodGoodGoodExcellent
Gene amplification for subcloningNot recommendedGood/IGoodGoodGoodExcellent
Degenerated DNA (with mixed base insertion)Not recommendedGoodInquiry required (availability depends on sequence and modification)Not recommendedNot recommendedNot recommended
MutagenesisNot recommendedNot recommendedGoodGoodExcellentExcellent
Gene synthesisNot recommendedNot recommendedGoodInquiry required (availability depends on sequence and modification)ExcellentInquiry required (availability depends on sequence and modification)
Oligo longer than 51mersNot recommendedGoodExcellentNot recommendedExcellentNot recommended
Oligo longer than 71mersNot recommendedNot recommendedGoodNot recommendedExcellentNot recommended
Modification (terminal)Not recommendedNot recommendedGoodInquiry required (availability depends on sequence and modification)Inquiry required (availability depends on sequence and modification)Not recommended
Modification (insertion)Not recommendedInquiry required (availability depends on sequence and modification)GoodInquiry required (availability depends on sequence and modification)Inquiry required (availability depends on sequence and modification)Not recommended
Modification (Fluorescent)Not recommendedNot recommendedGoodInquiry required (availability depends on sequence and modification)Not recommendedNot recommended
Phosphorothioate Oligo (S-oligo)Not recommendedNot recommendedGoodNot recommendedNot recommendedNot recommended

Custom DNA Oligonucleotides Synthesis

If you have any questions or requirements, please contact us.

* Only for research. Not suitable for any diagnostic or therapeutic use.
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