Custom DNA Synthesis

Custom DNA Synthesis

BOC RNA provides custom DNA synthesis services to help customers save time and money. All customized DNA oligonucleotides are produced under strict quality control standards, and all synthetic fragments are verified to ensure 100% sequence identity.

DNA synthesis service

  • Unmodified DNA synthesis

We can customize unmodified DNA in multiple scales, from small scale (customized DNA for research) to mass production of GMP DNA oligomers, at very competitive prices. Guaranteed yields for purified, unmodified DNA 20-50 bases in length:

100 nmol250 nmol1 µmol5 µmol10 µmol
PAGE Purification0.5-1 OD2 ODs10 ODs50 ODs100 ODs
HPLC Purification1-2OD4 ODs20 ODs100 ODs200 ODs
IE-HPLC Purification1-2OD4 ODs20 ODs100 ODs200 ODs
RNase-Free HPLC Purification1-2OD4 ODs20 ODs100 ODs200 ODs
Dual HPLC Purification0.5-1OD2 ODs10 ODs50 ODs100 ODs
Dual PAGE & HPLC0.5 OD1 OD5 ODs25 ODs50 ODs
  • Purity and yield may vary due to oligo length, modifications, or sequence composition.
  • DNA oligonucleotide modification

We also provide thousands of custom DNA modifications, including DNA backbone modifications, modified bases, and fluorescent dye labels. These modified oligonucleotides meet the highest quality specifications for research, diagnostics, OEM, and therapeutic drugs.

Modifications include:

5’ modification3' modificationInner modification
5' Phosphorylation3' PhosphorylationdT-Dabcyl
5' Biotin3' BiotindT-Bitoin
5' Digoxigenin3' DigoxigenindT-Digoxigenin
5' Amino linker C63' Amino linker C6dT C6-Aminolinker
5' Amino linker C123' Thiol C6Thiol-C6 S-S
5' Thiol C63' spacer C3Spacer 18
5' FITCA3' FITCPhospothioate
5' FAM3' FAMdU
5' TET3' TETdI
5' JOE3' JOE 
5' HEX3' HEX 
5' TAMRA3' TAMRA 
5' ROX3' ROX 
5' Cy-33' DABCYL
5' Cy-53' BHQ1
5' Cy-3.53' BHQ2
5' Thiol-C6 S-S3' Thiol-C6 S-S
5’ Fluorescein3’ Fluorescein

More modifications

Optional DNA synthesis services

  • Large scale oligonucleotide productions

Quality control

  • Use SEC chromatography to deprotect and desalt DNA oligonucleotides to remove impurities caused by trace molecules.
  • Quantify olionucleotide twice by ultraviolet spectrophotometry to provide an accurate OD yeid measurement.

To meet different experimental requirements, you can choose from the following different purification methods:

Gel FiltrationReverse-phase Cartridge PurificationHPLC PurificationIEX HPLC PurificationPAGE PurificationDouble Purifications
Gene amplificationGoodGoodGoodGoodGoodExcellent
Sequencing primersNot recommendedGoodGoodGoodGoodExcellent
Gene amplification for subcloningNot recommendedGood/IGoodGoodGoodExcellent
Degenerated DNA (with mixed base insertion)Not recommendedGoodInquiry required (availability depends on sequence and modification)Not recommendedNot recommendedNot recommended
MutagenesisNot recommendedNot recommendedGoodGoodExcellentExcellent
Gene synthesisNot recommendedNot recommendedGoodInquiry required (availability depends on sequence and modification)ExcellentInquiry required (availability depends on sequence and modification)
Oligo longer than 51mersNot recommendedGoodExcellentNot recommendedExcellentNot recommended
Oligo longer than 71mersNot recommendedNot recommendedGoodNot recommendedExcellentNot recommended
Modification (terminal)Not recommendedNot recommendedGoodInquiry required (availability depends on sequence and modification)Inquiry required (availability depends on sequence and modification)Not recommended
Modification (insertion)Not recommendedInquiry required (availability depends on sequence and modification)GoodInquiry required (availability depends on sequence and modification)Inquiry required (availability depends on sequence and modification)Not recommended
Modification (Fluorescent)Not recommendedNot recommendedGoodInquiry required (availability depends on sequence and modification)Not recommendedNot recommended
Phosphorothioate Oligo (S-oligo)Not recommendedNot recommendedGoodNot recommendedNot recommendedNot recommended

Service process

Custom DNA Synthesis

Competitive advantages

  • Flexible synthesis scales: 20nmol, 50 nmol, 100 nmol, 200 nmol, 1 µmol, 5 µmol, and 10 µmol. Bulk orders are available upon request
  • Experienced team: scientists and staff with over 15 years of experience in oligo synthesis and modifications
  • High purity: purification by PAGE or RP-HPLC upon request
  • High quality: QC analysis by MS, HPLC, or PAGE
  • Competitive prices
  • Fast delivery

If you have any questions or requirements, please contact us.

* Only for research. Not suitable for any diagnostic or therapeutic use.
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