BOC RNA provides custom DNA synthesis services to help customers save time and money. All customized DNA oligonucleotides are produced under strict quality control standards, and all synthetic fragments are verified to ensure 100% sequence identity.
DNA oligonucleotides are short fragments of DNA composed of a few nucleotide units. These units are linked together via phosphodiester bonds. Oligonucleotides may range from fewer than 10 nucleotides up to 100 nucleotides in length.
There are two major types of DNA oligonucleotides: synthetic and natural. Synthetic oligonucleotides are created in the laboratory, while natural oligonucleotides are produced by organisms.
DNA oligonucleotides have a broad range of applications in various fields. In the medical field, they are used for gene therapy and diagnostics. In molecular biology, they are used for sequencing, gene synthesis, and genetic engineering. In nanotechnology, DNA oligonucleotides are used as building blocks for creating nano-structures and devices.
The synthesis of DNA oligonucleotides is a complex process that requires specialized equipment and expertise. It can be achieved by automated synthesis or solid-phase synthesis, both of which utilize chemically modified nucleotides and various reagents.
We can customize unmodified DNA in multiple scales, from small scale (customized DNA for research) to mass production of GMP DNA oligomers, at very competitive prices. Guaranteed yields for purified, unmodified DNA 20-50 bases in length:
| 100 nmol | 250 nmol | 1 µmol | 5 µmol | 10 µmol | |
| PAGE Purification | 0.5-1 OD | 2 OD | 10 OD | 50 OD | 100 OD |
| HPLC Purification | 1-2 OD | 4 OD | 20 OD | 100 OD | 200 OD |
| IE-HPLC Purification | 1-2 OD | 4 OD | 20 OD | 100 OD | 200 OD |
| RNase-Free HPLC Purification | 1-2 OD | 4 OD | 20 OD | 100 OD | 200 OD |
| Dual HPLC Purification | 0.5-1 OD | 2 OD | 10 OD | 50 OD | 100 OD |
| Dual PAGE & HPLC | 0.5 OD | 1 OD | 5 OD | 25 OD | 50 OD |
| Purity and yield may vary due to oligo length, modifications, or sequence composition. | |||||
We also provide thousands of custom DNA modifications, including DNA backbone modifications, modified bases, and fluorescent dye labels. These modified oligonucleotides meet the highest quality specifications for research, diagnostics, OEM, and therapeutic drugs.
Modifications include:
| 5' modification | 3' modification | Inner modification |
| 5' Phosphorylation | 3' Phosphorylation | dT-Dabcyl |
| 5' Biotin | 3' Biotin | dT-Bitoin |
| 5' Digoxigenin | 3' Digoxigenin | dT-Digoxigenin |
| 5' Amino linker C6 | 3' Amino linker C6 | dT C6-Aminolinker |
| 5' Amino linker C12 | 3' Thiol C6 | Thiol-C6 S-S |
| 5' Thiol C6 | 3' spacer C3 | Spacer 18 |
| 5' FITCA | 3' FITC | Phospothioate |
| 5' FAM | 3' FAM | dU |
| 5' TET | 3' TET | dI |
| 5' JOE | 3' JOE | |
| 5' HEX | 3' HEX | |
| 5' TAMRA | 3' TAMRA | |
| 5' ROX | 3' ROX | |
| 5' Cy-3 | 3' DABCYL | |
| 5' Cy-5 | 3' BHQ1 | |
| 5' Cy-3.5 | 3' BHQ2 | |
| 5' Thiol-C6 S-S | 3' Thiol-C6 S-S |
To meet different experimental requirements, you can choose from the following different purification methods:
| Gel Filtration | Reverse-phase Cartridge Purification | HPLC Purification | IEX HPLC Purification | PAGE Purification | Double Purifications | |
| Gene amplification | Good | Good | Good | Good | Good | Excellent |
| Sequencing primers | Not recommended | Good | Good | Good | Good | Excellent |
| Gene amplification for subcloning | Not recommended | Good/I | Good | Good | Good | Excellent |
| Degenerated DNA (with mixed base insertion) | Not recommended | Good | Inquiry required (availability depends on sequence and modification) | Not recommended | Not recommended | Not recommended |
| Mutagenesis | Not recommended | Not recommended | Good | Good | Excellent | Excellent |
| Gene synthesis | Not recommended | Not recommended | Good | Inquiry required (availability depends on sequence and modification) | Excellent | Inquiry required (availability depends on sequence and modification) |
| Oligo longer than 51mers | Not recommended | Good | Excellent | Not recommended | Excellent | Not recommended |
| Oligo longer than 71mers | Not recommended | Not recommended | Good | Not recommended | Excellent | Not recommended |
| Modification (terminal) | Not recommended | Not recommended | Good | Inquiry required (availability depends on sequence and modification) | Inquiry required (availability depends on sequence and modification) | Not recommended |
| Modification (insertion) | Not recommended | Inquiry required (availability depends on sequence and modification) | Good | Inquiry required (availability depends on sequence and modification) | Inquiry required (availability depends on sequence and modification) | Not recommended |
| Modification (Fluorescent) | Not recommended | Not recommended | Good | Inquiry required (availability depends on sequence and modification) | Not recommended | Not recommended |
| Phosphorothioate Oligo (S-oligo) | Not recommended | Not recommended | Good | Not recommended | Not recommended | Not recommended |
If you have any questions or requirements, please contact us.
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