We specialize in Custom DNA Oligonucleotides Synthesis, offering high-quality, tailored DNA oligos for research and diagnostic applications. Our services cater to a wide range of needs, from basic research to complex therapeutic developments. We provide precise oligo synthesis with flexible scales, including standard, modified, and labeled sequences. With advanced technology and a commitment to quality, we ensure fast turnaround times, excellent purity, and cost-effective solutions. Whether you're conducting PCR, gene synthesis, or siRNA research, our Custom DNA Oligonucleotides Synthesis service is designed to meet your specific needs with accuracy and reliability. Contact us today for the best in oligo synthesis.
We can customize unmodified DNA in multiple scales, from small scale (customized DNA for research) to mass production of GMP DNA oligomers, at very competitive prices. Guaranteed yields for purified, unmodified DNA 20-50 bases in length:
| 100 nmol | 250 nmol | 1 µmol | 5 µmol | 10 µmol | |
| PAGE Purification | 0.5-1 OD | 2 OD | 10 OD | 50 OD | 100 OD |
| HPLC Purification | 1-2 OD | 4 OD | 20 OD | 100 OD | 200 OD |
| IE-HPLC Purification | 1-2 OD | 4 OD | 20 OD | 100 OD | 200 OD |
| RNase-Free HPLC Purification | 1-2 OD | 4 OD | 20 OD | 100 OD | 200 OD |
| Dual HPLC Purification | 0.5-1 OD | 2 OD | 10 OD | 50 OD | 100 OD |
| Dual PAGE & HPLC | 0.5 OD | 1 OD | 5 OD | 25 OD | 50 OD |
| Purity and yield may vary due to oligo length, modifications, or sequence composition. | |||||
We also provide thousands of custom DNA modifications, including DNA backbone modifications, modified bases, and fluorescent dye labels. These modified oligonucleotides meet the highest quality specifications for research, diagnostics, OEM, and therapeutic drugs.
Modifications include:
| 5' modification | 3' modification | Inner modification |
| 5' Phosphorylation | 3' Phosphorylation | dT-Dabcyl |
| 5' Biotin | 3' Biotin | dT-Bitoin |
| 5' Digoxigenin | 3' Digoxigenin | dT-Digoxigenin |
| 5' Amino linker C6 | 3' Amino linker C6 | dT C6-Aminolinker |
| 5' Amino linker C12 | 3' Thiol C6 | Thiol-C6 S-S |
| 5' Thiol C6 | 3' spacer C3 | Spacer 18 |
| 5' FITCA | 3' FITC | Phospothioate |
| 5' FAM | 3' FAM | dU |
| 5' TET | 3' TET | dI |
| 5' JOE | 3' JOE | |
| 5' HEX | 3' HEX | |
| 5' TAMRA | 3' TAMRA | |
| 5' ROX | 3' ROX | |
| 5' Cy-3 | 3' DABCYL | |
| 5' Cy-5 | 3' BHQ1 | |
| 5' Cy-3.5 | 3' BHQ2 | |
| 5' Thiol-C6 S-S | 3' Thiol-C6 S-S |
To meet different experimental requirements, you can choose from the following different purification methods:
| Gel Filtration | Reverse-phase Cartridge Purification | HPLC Purification | IEX HPLC Purification | PAGE Purification | Double Purifications | |
| Gene amplification | Good | Good | Good | Good | Good | Excellent |
| Sequencing primers | Not recommended | Good | Good | Good | Good | Excellent |
| Gene amplification for subcloning | Not recommended | Good/I | Good | Good | Good | Excellent |
| Degenerated DNA (with mixed base insertion) | Not recommended | Good | Inquiry required (availability depends on sequence and modification) | Not recommended | Not recommended | Not recommended |
| Mutagenesis | Not recommended | Not recommended | Good | Good | Excellent | Excellent |
| Gene synthesis | Not recommended | Not recommended | Good | Inquiry required (availability depends on sequence and modification) | Excellent | Inquiry required (availability depends on sequence and modification) |
| Oligo longer than 51mers | Not recommended | Good | Excellent | Not recommended | Excellent | Not recommended |
| Oligo longer than 71mers | Not recommended | Not recommended | Good | Not recommended | Excellent | Not recommended |
| Modification (terminal) | Not recommended | Not recommended | Good | Inquiry required (availability depends on sequence and modification) | Inquiry required (availability depends on sequence and modification) | Not recommended |
| Modification (insertion) | Not recommended | Inquiry required (availability depends on sequence and modification) | Good | Inquiry required (availability depends on sequence and modification) | Inquiry required (availability depends on sequence and modification) | Not recommended |
| Modification (Fluorescent) | Not recommended | Not recommended | Good | Inquiry required (availability depends on sequence and modification) | Not recommended | Not recommended |
| Phosphorothioate Oligo (S-oligo) | Not recommended | Not recommended | Good | Not recommended | Not recommended | Not recommended |
We specialize in the synthesis of long DNA oligonucleotides (up to 200 bases or more). These are particularly useful for gene synthesis, cloning, and other applications requiring longer sequences.
Our technology allows us to handle high GC content oligos with improved yield and performance. This is especially important for applications like PCR or primer design where GC-rich sequences are often involved.
We offer a variety of purification options, from desalted oligos for routine applications to HPLC-purified or PAGE-purified oligos for high-quality or sensitive experiments requiring the highest purity.
If your oligos degrade or need modifications after initial synthesis, we can assist with resynthesis or re-amplification to ensure your research progresses smoothly.
In addition to DNA oligos, we also provide custom RNA oligonucleotides for applications like siRNA, mRNA synthesis, or RNA-based assays, enabling advanced studies in gene silencing and expression.
For commercial projects or large research studies, we provide bulk oligonucleotide synthesis in large quantities. Our scale-up capabilities ensure that you receive consistent, high-quality oligos for your entire project.
Start by providing your desired DNA or RNA sequence, including any specific modifications, labels, or purification requirements. Our intuitive online order form makes it easy to submit your custom oligo request, ensuring all necessary details are captured for accurate synthesis.
Our scientific team reviews your sequence to verify feasibility and suggest any optimizations if necessary. This step ensures that even complex sequences with high GC content or unusual modifications are suitable for synthesis, maximizing the success of your experiments.
You will receive a transparent quote based on oligo length, modifications, purification, and quantity. Once approved, we confirm your order and schedule it for synthesis, keeping you informed every step of the way.
Using state-of-the-art DNA/RNA synthesis technology, we produce your oligos with high accuracy and reproducibility. Our process supports a wide range of modifications, including fluorescent tags, biotin labels, phosphorothioate linkages, and more.
After synthesis, oligos undergo purification according to your selected method (desalted, HPLC, or PAGE) and stringent quality control to ensure high purity, correct sequence, and functional integrity. Each batch is tested to meet the standards required for reliable experimental results.
Your oligos are packaged safely for shipment and accompanied by a Certificate of Analysis (COA), detailing sequence, purity, and other critical specifications. This documentation ensures traceability and confidence in your research materials.
We offer fast, reliable shipping worldwide, including expedited options for urgent projects. Customers receive tracking information and estimated delivery times, ensuring that your oligos arrive promptly and in perfect condition.
Our Custom DNA Oligonucleotides Synthesis is ideal for PCR, gene cloning, sequencing, and other molecular biology applications. Whether you need primers for PCR or probes for sequencing, we can provide high-quality oligos that ensure accuracy and efficiency in your research.
In genomics and gene synthesis, precise oligos are essential. Our service supports applications like gene assembly, CRISPR gene editing, and genome sequencing, providing high-quality oligos tailored for genetic research and innovation.
For RNA interference (RNAi) studies, including siRNA and shRNA applications, we provide custom RNA oligos that are essential for efficient gene silencing. These oligos are carefully designed to ensure optimal performance in gene knockdown experiments.
Our oligos are ideal for diagnostic assay development, including real-time PCR, microarrays, and DNA probes. We support applications that require specific sequences for disease detection and biomarker validation, ensuring high accuracy and sensitivity.
In drug discovery and gene therapy, oligonucleotides play a critical role in developing therapeutic agents. Our oligo synthesis service supports the development of antisense oligonucleotides, RNA-based therapeutics, and vaccine research, providing high-quality oligos for your pharmaceutical projects.
Synthetic biology requires custom DNA oligos for creating genetic circuits, optimizing metabolic pathways, and more. Our service supports these applications by providing accurate and reliable oligo synthesis, helping to advance industrial biotechnology, biofuel production, and other commercial ventures.
Custom DNA Oligonucleotide Synthesis is a service where oligonucleotides (short DNA or RNA sequences) are synthesized according to specific customer requirements. These sequences can be tailored for various applications such as PCR, gene synthesis, RNA interference, and diagnostic assays.
We can synthesize a wide range of DNA oligos, including:
Standard DNA oligos for PCR, sequencing, and cloning.
Modified oligos with chemical modifications (e.g., phosphorothioates, amino acids).
Fluorescent and biotinylated oligos for sensitive detection applications.
Long oligos for gene synthesis or complex cloning applications.
RNA oligos for RNAi or other RNA-based experiments.
Yes, we offer a variety of modifications, such as:
5' or 3' labeling with fluorescent dyes or biotin.
Internal modifications such as LNA (Locked Nucleic Acid) or methylations.
Phosphorothioate linkages for enhanced stability.
Dual-labeled probes for quantitative applications.
We offer several oligo purification methods:
Desalted: Suitable for routine applications where high purity is not a primary concern.
HPLC-purified: For high-quality oligos where minimal contaminants are required.
PAGE-purified: For the highest level of purity, especially in sensitive applications like probe design or critical experiments.
To place an order, simply fill out our online order form, specifying your required sequence, modifications, and purification preferences. If you have any questions or need assistance with the order, our customer service team is available to help.
Yes, we specialize in synthesizing complex sequences, including those with high GC content, secondary structures, and unusual modifications. Our technology ensures that even the most challenging sequences are synthesized with precision.
Yes, we offer bulk oligonucleotide synthesis for large-scale orders. Whether you need hundreds or thousands of oligos, we can ensure consistent quality and reliable delivery for your large projects or commercial applications.
Yes, we offer custom RNA oligonucleotides synthesis for various applications such as RNA interference (siRNA, shRNA), mRNA synthesis, and other RNA-based research needs.
Ready to Accelerate Your Research with High-Quality Custom DNA Oligos?
Unlock precise, reliable, and fully customized DNA oligonucleotides for your molecular biology, genomics, or therapeutic projects. Order now or contact our experts to discuss your sequence design, modifications, and purification options. With fast turnaround times, competitive pricing, and expert support, we make it easy to get the oligos you need-exactly when you need them. Don't let oligo limitations slow your research—start your custom DNA synthesis today!
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