Custom PNA Oligonucleotides Synthesis

Peptide Nucleic Acids (PNAs) represent a transformative class of synthetic molecules that have revolutionized molecular biology and biotechnology. Developed as analogs to natural nucleic acids, PNAs feature a unique backbone structure that combines the nucleobases of DNA or RNA with a peptide-like backbone. BOC Sciences, with over two decades of expertise in the biotechnology industry, stands at the forefront of custom PNA oligonucleotide synthesis. Our commitment to excellence and innovation enables us to offer tailored PNA solutions that meet the precise needs of researchers and industry professionals worldwide. From consultation and sequence design to synthesis, purification, and delivery, BOC Sciences ensures the highest quality and reliability in every aspect of PNA synthesis.

What is PNA?

Peptide nucleic acids (PNAs) are synthetic mimics of DNA with a pseudo-peptide backbone, offering high affinity and specificity in hybridizing with complementary DNAs or RNAs due to their uncharged and flexible polyamide structure. These unique properties have led to the development of various research assays and diagnostic applications. PNAs have proven their utility in molecular biology procedures and are now used as molecular hybridization probes, antigene and anticancer drugs, PCR reaction modulators, genomic mutation detectors, and chromosome labeling tools.

PNA Structure and Properties

PNAs, introduced by Nielsen et al., feature a synthetic DNA analog structure with a pseudo-peptide backbone made of N-(2-aminoethyl) glycine units. These backbones provide PNAs with higher stability and a higher thermal melting temperature (Tm) compared to natural DNA or RNA duplexes. PNAs are also resistant to nucleases and proteases, extending their lifespan both in vivo and in vitro.

• Binding Affinity: Due to the neutral peptide backbone, PNAs exhibit stronger binding to complementary DNA and RNA sequences compared to natural nucleic acids. This high affinity is a result of the absence of electrostatic repulsion that is typically present in DNA-DNA or DNA-RNA duplexes.
• Specificity: PNAs can form highly specific and stable complexes with target nucleic acid sequences. This specificity reduces the likelihood of off-target effects, enhancing the accuracy of diagnostic assays and therapeutic interventions.
• Versatility: The unique properties of PNAs allow them to hybridize under a wide range of conditions, including low ionic strength environments. This versatility is advantageous for various experimental settings and applications.

Custom PNA Oligonucleotides Synthesis

BOC Sciences offers comprehensive custom PNA oligonucleotide synthesis services, leveraging advanced technologies and extensive expertise to deliver high-quality PNAs tailored to the precise requirements of our clients. Our service flow include:

Consultation and Design

Our team of experts collaborates with clients to understand their specific needs and design optimal PNA sequences. We offer consultation on sequence design, modification options, and potential applications to ensure the best outcomes.

Custom Synthesis

We provide fully customized PNA synthesis, accommodating specific sequence requirements, modifications, and scales. Our synthesis process ensures high purity and yield, adhering to stringent quality control standards.

Modification and Labeling

BOC Sciences offers a range of modifications and labeling options, including fluorescent dyes, biotin, and other functional groups. These modifications enhance the utility of PNAs in various applications, such as imaging, detection, and immobilization.

Purification and Analysis

Our rigorous purification protocols, including HPLC and mass spectrometry, ensure that the synthesized PNAs meet the highest standards of purity and accuracy. Detailed analytical reports are provided with every order.

Quality Control

Each batch of synthesized PNA is subjected to comprehensive quality control measures, including mass spectrometry and analytical HPLC. This ensures that the final product meets the specified requirements and standards.

Delivery

The purified and quality-verified PNAs are securely packaged and delivered to clients, accompanied by detailed analytical reports. Our efficient logistics ensure timely delivery worldwide.

Applications of PNA Technology

PNAs have diverse applications across various fields, including:

Antisense and Antigene Applications

PNAs can inhibit gene transcription and translation by binding tightly to DNA or mRNA. They can form stable complexes with DNA, blocking transcriptional elongation and inhibiting transcription factor binding. PNAs also interact with mRNA, causing steric interference with RNA processing, transport, or translation.

PNA as Delivery Agents

PNAs can be used to enhance gene transfer efficiency in nonviral gene therapy by linking peptides, drugs, or tracers to plasmid vectors, aiding gene delivery and tissue-specific targeting.

PCR and Q-PNA PCR

PNAs can terminate the elongation of oligonucleotide primers in PCR reactions by binding to the template or competing with primers. PNA-directed PCR clamping uses PNAs to inhibit specific target amplification, useful for mutation screening and gene isolation.

Nucleic Acid Capture

PNAs are employed for sequence-specific capture of single-stranded nucleic acids, utilizing their tight complex formation at low ionic strength. This technique is useful for purifying nucleic acids and detecting specific DNA sequences.

Solid-Phase Hybridization Techniques

PNAs offer advantages in hybridization applications due to their tighter binding and higher specificity. They can accelerate procedures in standard hybridization techniques, such as Southern and Northern blotting, and pregel hybridization.

Peptide nucleic acids are versatile and powerful tools in molecular genetics and cytogenetics, with applications ranging from gene therapy to diagnostic assays. Their unique properties make them valuable for a wide range of molecular biology and medical applications.

Advantages of Our Service

Expertise and Experience

With over two decades of experience in the field, BOC Sciences boasts unparalleled expertise in PNA synthesis. Our team of skilled scientists and advanced technological infrastructure ensure the highest quality products and services.

Customization and Flexibility

We offer fully customizable synthesis services, accommodating specific sequence requirements, modifications, and scales. Our flexible approach allows us to meet the unique needs of each client, delivering tailored solutions.

Quality and Reliability

At BOC Sciences, quality is paramount. Our rigorous synthesis, purification, and quality control processes guarantee high purity and accuracy of the synthesized PNAs. Clients can trust in the reliability and consistency of our products.

Comprehensive Support

From initial consultation to final delivery, we provide comprehensive support throughout the entire process. Our dedicated team is committed to ensuring client satisfaction and successful project outcomes.

Competitive Pricing

We offer competitive pricing without compromising on quality. Our cost-effective solutions make advanced PNA technology accessible to a wide range of researchers and industries.

BOC Sciences is at the forefront of custom PNA oligonucleotide synthesis, providing high-quality, tailored solutions to meet the diverse needs of the scientific community. With our extensive expertise, advanced technologies, and commitment to excellence, we deliver PNAs that empower researchers and drive innovation in molecular biology, diagnostics, and therapeutics. For more customization services, please contact us directly.

Case Study Antisense Peptide Nucleic Acids in Neurodegenerative Disease Research

Therapeutic and diagnostic applications of antisense peptide nucleic acids. (MacLelland, V.; et al, 2024)

In a study focusing on Alzheimer's disease, antisense peptide nucleic acids (PNAs) were designed to target amyloid precursor protein (APP) mRNA. These PNAs were conjugated with cell-penetrating peptides to facilitate crossing the blood-brain barrier and delivery to neuronal cells. The treatment in a mouse model resulted in a significant reduction of amyloid-beta plaques, improved cognitive function, and decreased neuroinflammation. Despite these promising outcomes, challenges such as large-scale synthesis, potential off-target effects, and efficient delivery methods need to be addressed. This study highlights the potential of PNAs as a therapeutic approach for neurodegenerative diseases, underscoring the need for further development and optimization.

FAQ

1. What is PNA vaccine?

PNA vaccine refers to the use of PNA (peptide nucleic acid) as one of the core technologies of the vaccine. This technique utilizes PNA molecules to design and synthesize vaccines, which are commonly used to improve immune response and enhance vaccine stability and efficacy.

2. What are antisense peptide nucleic acids?

Antisense peptide nucleic acids (PNAs) are gene expression modification techniques that block the translation process of RNA by binding to complementary sequences of target genes. PNAs are different from DNA or RNA in that their skeleton is composed of N-(2-aminoethyl) glycine units, which are highly chemically stable and selective. While, antisense oligonucleotides (ASOs) are short DNA or RNA molecules that are able to bind to complementary sequences of target RNA to form double - or triple-stranded structures. This binding can block the translation of the target RNA or enhance its degradation, thereby regulating the expression of the target gene. ASO has important application value in gene silencing, gene modification and treatment of genetic diseases.

3. What is the function of the PNA?

PNA (peptide nucleic acid) is a synthetic DNA simulator with high chemical stability and biological activity. Its primary function is as a analog of DNA and RNA in molecular biology research, for exploration of genomics, drug discovery, and the development of diagnostic tools.

4. What are peptide nucleic acid probes?

A peptide nucleic acid probe (PNA probe) is a synthetic nucleic acid analogue that has peptide bonds rather than glycoside bonds to connect bases. The probe can bind specifically to DNA or RNA sequences for molecular biology research and diagnostic applications.

5. What is a nucleic acid probe?

A nucleic acid probe is a short strand of DNA or RNA that is used to detect a specific sequence of nucleic acids. They recognize and detect the presence and location of the target DNA or RNA by binding specifically to the target sequence. Nucleic acid probes are widely used in molecular biology and medical diagnostics to help scientists study gene function, detect pathogens, and diagnose genetic diseases.

Reference

1. MacLelland, V.; et al. Therapeutic and Diagnostic Applications of Antisense Peptide Nucleic Acids. Mol Cell. 2024, 35(1): 102086.

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