As a professional oligonucleotide synthesis company, BOC RNA has designed and built a standard plate oligonucleotide synthesis solution. Our advanced and innovative manufacturing process ensures you get a high-quality product. In addition, our scientists will provide you with free consultation and technical support for your research needs in molecular biology, diagnostics and drug discovery.
Oligonucleotides are short single-stranded fragments of nucleic acids that can easily bind to their respective complementary fragments in a sequence specific manner to form double-stranded bodies. Oligonucleotides are usually synthesized in vitro by solid-phase chemistry synthesis.
As early as the early 1990s, DA Lashkari et al. developed a 96-well plate-based automated multiplex oligonucleotide synthesizer (96-well-plate-AMOS). AMOS utilizes 5'-protected deoxynucleosides anchored to the controlled pore glass matrix (which is in a modified 96-well plate enclosed in an argon flow chamber) support at the 3' -terminal through chemically cleavable linker. Then a series of phosphamide synthesis chemistry was carried out to rapidly synthesize up to 96 different oligonucleotides simultaneously in 96-well microtitration. Upon completion of the strand assembly process, the oligonucleotide is released from the solid support and eluted from the column or pore. Finally, the quality of oligonucleotide was evaluated by capillary electrophoresis (CE) combined with High Performance Liquid Chromatography (HPLC), and the amount of oligonucleotide product was measured by mass spectrometry (MS).
Subsequently, more advanced oligonucleotide synthesizers were constructed, such as MerMade (oligos synthesizer for high-throughput production of oligonucleotide in dual 96-well plates), NG-1536-AMOS (1536 well plate oligonucleotide synthesizer). Their emergence further reduces the production cost while meeting the need for higher throughput oligonucleotide synthesis.
Service Options | Product | Synthesis Scale | Length | Price |
Custom DNA Plate Oligo | Single-stranded DNA Plate Oligo | 10 nmol | 15 - 60 Bases | Inquiry |
25 nmol | 15 - 60 Bases | Inquiry | ||
50 nmol | 10 - 90 Bases | Inquiry | ||
100 nmol | 10 - 90 Bases | Inquiry | ||
Primer mix DNA Plate Oligo | 10 nmol | 15 - 60 Bases | Inquiry | |
25 nmol | 15 - 60 Bases | Inquiry | ||
50 nmol | 10 - 90 Bases | Inquiry | ||
100 nmol | 10 - 90 Bases | Inquiry | ||
Duplexed DNA Plate Oligo | 50 nmol | 10 - 90 Bases | Inquiry | |
100 nmol | 10 - 90 Bases | Inquiry | ||
250 nmol | 5 - 100 Bases | Inquiry | ||
Custom RNA Plate Oligo | Single-stranded RNA Plate Oligo | 10 nmol | 5-60 bases | Inquiry |
25 nmol | 5-60 bases | Inquiry | ||
50 nmol | 5-60 bases | Inquiry |
If you have any needs, please feel free to contact us.
The concept of "plate-based oligonucleotides" is not only applied to production but also to detection and quantification. For example:
Fig. 1 Principles of OLISA (Han K C, 2010)
Fig. 2 Illustration of the POE immunoassay sandwich (Han K C, 2020)
References