Custom Oligo Synthesis

BOC RNA provides high purity customized oligonucleotide production services with a variety of chemistry modifications and specifications, which can be constructed according to your application to meet the research needs in biology, diagnostics, and drug discovery.

Introduction

Oligonucleotides are a general term for a class of short-chain nucleotides with less than 20 base pairs, oligonucleotides can be oligodeoxyribonucleotides or oligoribonucleotides. As a class of nucleic acid molecules with short sequences, oligonucleotides are basic tools for regulating gene expression in life science and biomedical research.

Applications

• Oligonucleotide primers

As primers, oligonucleotides are often used to initiate enzymatic reactions (e.g. oligosaccharides) to generate millions to billions of copies of short or long target sequences. Well-known examples are polymerase chain reaction (PCR) or Sanger sequencing methods. Applications of oligonucleotide primers include DNA sequencing, gene expression, cloning and molecular diagnostics.

• Oligonucleotide probes

As probes, oligonucleotides are used to identify and bind to a specific DNA or RNA target sequence to confirm the presence of that sequence in a specific material. Applications using oligonucleotide probes include blotting procedures, such as Northern blotting (for RNA) or Southern blotting (for DNA), as fluorophore-conjugated sequences in microarrays to detect changes in gene expression, or for screening genetic diseases or identifying specific pathogens (molecular diagnostics).

• Oligonucleotide therapy/gene therapy

In therapeutic applications, antisense oligonucleotides (ASO) use natural biology to promote gene repression or gene silencing (disruption) of undesirable or overactive RNA sequences, thereby blocking the expression of certain damaged or overactive proteins that have the potential to cause or induce disease.

Oligonucleotide synthesis services

Why Us

• Oligonucleotides customization

BOC RNA uses phosphoramidite solid-phase synthesis to synthesize unmodified or modified oligonucleotides with specific sequences and provides large-scale customized unmodified oligonucleotide synthesis.

• Purification

Each oligonucleotide is strictly monitored during the synthesis process and controlled under BOC RNA's strict quality assurance and quality control standards. The final product is identified by mass spectrometry, and its purity is analyzed by high-throughput electrophoresis or HPLC according to purification options to achieve the required purity.

• Modifications/labeling

BOC RNA provides comprehensive modifications for your research needs, including

For more details, please refer to our oligo modifications service.

• Available scales

Flexible Synthesis Scales: 20 nmol, 50 nmol, 200 nmol, 1μmol, 5μmol and 10μmol. Bulk orders are available upon request.

Standard Oligo Synthesis Process