In the development of mRNA vaccines, the design and development of delivery systems is a prerequisite for efficient cytoplasmic delivery and maximization of efficacy. Here, BOC Sciences offers cationic nanoemulsion-based mRNA delivery systems, promising a one-stop service from mRNA synthesis to cationic nanoemulsion encapsulation to efficiently assist our customers in vaccine development and production.
Cationic nanoemulsions are emulsion colloidal systems consisting of oil or lipid droplets dispersed in an aqueous solution with the addition of a cationic surfactant, where the size range of the dispersed phase droplets is typically less than 100 nm. Squalene-based cationic nanoemulsions have been shown to deliver mRNA with good stability and efficient cellular uptake. The nanoemulsions have squalene as the core and include a shell surrounded by cationic lipids (e.g. DOTAP) and surfactants (e.g. Tween 80 and Span 85). mRNA is then adsorbed to its surface by electrostatic binding.
Figure 1. Squalene-based cationic nanoemulsion carriers, where mRNA is adsorbed to the surface by electrostatic binding. (Namit, C.; et al.2021)
Compared to liposomes, cationic nanoemulsion carriers exhibit higher transfection and lower toxicity.
(1)Relatively low cost: cationic nanoemulsions can be produced on an industrial scale at a relatively low cost.
(2)Enhanced mRNA stability: Cationic nanoemulsions provide a protective layer that extends the stability and storage life of the drug.
(3)Improved cellular uptake: The cationic portion of the cationic surfactant interacts with the negative charge of the cell membrane, promoting adsorption and cellular uptake of the nanoemulsion.
(4)High biocompatibility: Many cationic surfactants have good biocompatibility and produce low toxicity and irritation to living organisms.
(5)Functional adjustability: Cationic nanoemulsions can be adjusted to achieve a variety of functions by adjusting surface properties and changing formulations.
BOC Sciences' experts are dedicated to providing design, preparation and development services for mRNA delivery systems based on cationic nanoemulsion carriers according to customer needs. For the design of cationic nanoemulsion carriers our core focus is on squalene, and ice is provided with a choice of cationic surfactants. Of course other cationic nanoemulsion customization services can be realized at BOC Sciences. You can contact us directly with any ideas you may have and let BOC Sciences support you.
Figure 2. Preparation process of cationic nanoemulsions.
Figure 3. In vitro&in vivo validation of cationic nanoemulsion-mRNA.
|Squalene-based Cationic Nanoemulsion
|Squalene-based cationic nanoemulsions use the natural hydrocarbon squalene as the oil phase and incorporate a cationic surfactant to provide a positive charge on the droplet surface. Squalene-based cationic nanoemulsions have the advantages of excellent biocompatibility, enhanced cellular uptake, and high stability.
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