Spacer Modifiers

BOC RNA offers a variety of different spacer modifiers for DNA and RNA oligonucleotide modifications. These spacers have different atomic numbers and are typically used to create distances between the functional part and the hybridized region of the oligonucleotide. With our extensive experience in nucleotide chemistry, we repeatedly control and check the entire manufacturing process at each stage to achieve high quality levels.

Type of Spacer Modifiers

• C3/C6 Spacer

C3 refers to 3 CH₂ and C6 refers to 6 CH₂, which acts as a linker to increase the spatial distance, and can be modified at the 5 end, the 3 end, and the middle, and more than one middle can be modified. When modified at the 3' end of the primer, it acts as a blocker to prevent the extension of DNA polymerase.

• Spacer 9/Spacer 18

Spacer 9/18 is a linker chain of three/six consecutive glycols (CH₂CH₂O-), which can be modified at the 5 end, 3 end and the middle of the chain. Spacer 18 is commonly used to introduce a strong hydrophobic group.

• PC Spacer /PC Linker

The PC (Photo-Cleavable) spacer is placed between the DNA bases. It can be broken under UV irradiation in the 300-350 nm spectral range. It releases Oligo with a 5'-phosphate group.

• dspacer THF

It is used to introduce an empty deoxyribonucleotide without a base at a nucleotide site because the site does not contain a base and cannot form a hydrogen bond, also known as the AP site (abasic site). Commonly used for RPA probes.

Advantages of Spacer Modifications

• Spacer can reduce spatial interactions to increase hybridization of oligonucleotides bound to support .
• Spacer can reduce interactions by increasing the distance.
• C3 spacers can be used to simulate oligonucleotide base mimicry
• dSpacer-modified oligonucleotides can be used to mimic base-free sites within oligonucleotides.

We Provide the Following Spacer Modifiers

Advantages

• Monitor the process from material procurement to final quality control and product release, providing batch-to-batch reproducibility
• Flexible packaging. 10, 20 and 50 nmol scale oligonucleotides. Custom packaging and bulk packaging are available.
• Our extensive experience in oligonucleotide chemical synthesis, exclusive equipment, reagents and expertise ensure the high quality of each oligonucleotide.
• One-on-one service. Real-time technical guidance from a team of experienced experts.
• More affordable options. We offer competitive pricing throughout the industry.

The expert team at BOC RNA has extensive experience in the synthesis and modification of custom oligonucleotides. We are committed to providing end-to-end customized DNA/RNA modification services, from consulting, DNA/RNA oligonucleotide design, production and purification, to quality control and delivery. Please contact us for more details.