BOC RNA offers a variety of different spacer modifiers for DNA and RNA oligonucleotide modifications. These spacers have different atomic numbers and are typically used to create distances between the functional part and the hybridized region of the oligonucleotide. With our extensive experience in nucleotide chemistry, we repeatedly control and check the entire manufacturing process at each stage to achieve high quality levels.
C3 refers to 3 CH2 and C6 refers to 6 CH2, which acts as a linker to increase the spatial distance, and can be modified at the 5 end, the 3 end, and the middle, and more than one middle can be modified. When modified at the 3' end of the primer, it acts as a blocker to prevent the extension of DNA polymerase.
Spacer 9/18 is a linker chain of three/six consecutive glycols (CH2CH2O-), which can be modified at the 5 end, 3 end and the middle of the chain. Spacer 18 is commonly used to introduce a strong hydrophobic group.
The PC (Photo-Cleavable) spacer is placed between the DNA bases. It can be broken under UV irradiation in the 300-350 nm spectral range. It releases Oligo with a 5'-phosphate group.
It is used to introduce an empty deoxyribonucleotide without a base at a nucleotide site because the site does not contain a base and cannot form a hydrogen bond, also known as the AP site (abasic site). Commonly used for RPA probes.
| Spacer Modifiers | Short Code | Price |
| C18 Spacer | 18S | Inquiry |
| C3 Spacer | C3 | Inquiry |
| C9 Spacer | 9S | Inquiry |
| dSpacer | dab | Inquiry |
| rSpacer | rab | Inquiry |
The expert team at BOC RNA has extensive experience in the synthesis and modification of custom oligonucleotides. We are committed to providing end-to-end customized DNA/RNA modification services, from consulting, DNA/RNA oligonucleotide design, production and purification, to quality control and delivery. Please contact us for more details.
Spacer modifiers are chemical linkers used in oligonucleotide synthesis to create distance between functional groups and the hybridized region. They help optimize hybridization efficiency and enable more precise control in experiments like DNA amplification and sequencing.
C3 and C6 spacers, made up of 3 or 6 CH2 units, are used to increase the spatial distance in oligonucleotides. These spacers are commonly applied at the 5' or 3' end to prevent unwanted DNA polymerase extension or to modify the oligonucleotide's structural properties.
Spacer 9 (three glycols) and Spacer 18 (six glycols) are often used to introduce hydrophilic or hydrophobic groups into the oligonucleotide structure. Spacer 18 is particularly useful for modifying the oligonucleotide's chemical properties and improving its stability and interaction efficiency in assays.
A PC (Photo-Cleavable) spacer is used in oligonucleotides to facilitate controlled cleavage under UV light (300-350 nm). It is typically used to release oligonucleotides with a 5'-phosphate group, making it valuable in applications like gene synthesis, PCR, and sequencing.
A dSpacer is a deoxyribonucleotide site without a base, known as an abasic (AP) site. It is commonly used in experiments that require the introduction of a base-free site, such as in RPA (recombinase polymerase amplification) probes, to simulate gaps in DNA sequences.
BOC RNA employs strict quality control measures throughout the entire manufacturing process, from material procurement to final product release. Our expert team monitors each stage to ensure high-quality, reproducible results for your oligonucleotide modifications.
