Methylene Blue Labeling of Oligonucleotides

BOC Sciences' oligonucleotide modification services include a wide range of labeling options, providing customers with personalized solutions to meet their specific needs. With a wide range of options to choose from, we offer the right labeling service. We will help you select the appropriate labeling method and optimize the labeling method according to and for your specific needs.

Fig 1. Synthesis of methylene blue oligonucleotide conjugates. (De Laet et al., 2016)

What is Methylene Blue?

Methylene Blue belongs to the phenothiazine family of dyes and is a unique dye with many useful properties. Despite its high extinction coefficient in the visible region, it is weakly fluorescent due to its high intersystem crossing rate from the S1 excited state to the T1 triplet state. This property makes it an excellent photosensitizer and has been widely used for the production of highly reactive single linear oxygen species. Methylene blue-labeled oligonucleotides can be directly observed by common visualization methods such as gel electrophoresis. The methylene blue labeling method is applicable to various types of oligonucleotides, including DNA and RNA oligonucleotides, as well as oligonucleotides with modifying groups.

Methylene Blue Labeling Oligonucleotide Services

BOC Sciences can provide custom synthesis services for methylene blue labeled oligonucleotides. When designing an experiment, we need to select the length and type of oligonucleotide according to the customer's requirements, and then design a suitable experimental protocol. After completing the custom synthesis, the product needs to be characterized and validated for application to ensure that the product obtained is the target product. Throughout the process, strict quality control is required to ensure the consistency and high quality of the product.

Methylene Blue Labeled Oligonucleotide Service Process

• The customer submits the oligonucleotide labeling synthesis request and synthesis requirements, agrees with the company on the specific oligonucleotide parameters and synthesis price, and both parties sign a contract.
• The customer provides the oligonucleotide sequence, we arrange the synthesis and purification experiments of methylene blue labeled oligonucleotide according to the agreement, and complete the synthesis commission within the specified time.
• After the completion of the synthesis commission, we will return the product to the customer and provide the synthesis report at the same time.

Sample Requirements

• The samples to be labeled should be over 90% pure, and the samples should not contain any components that interfere with the binding of the marker. If there are any special components, please mark them in advance.
• The minimum labeling amount of the sample to be labeled is 1 mg, if it is liquid, the concentration should be more than 1 mg/mL.
• The concentration of the sample should be suitable for the labeling reaction. Ensure that the sample concentration provides sufficient signal intensity in the labeling reaction while avoiding problems caused by over-dilution or excessive concentration.
• Use appropriate buffers to maintain proper pH and ionic strength in the labeling reaction. Commonly used buffers include sodium bicarbonate buffer, phosphate buffer, etc. The specific choice should be based on the experimental requirements and optimal conditions for the labeling reaction.

BOC Sciences provides oligonucleotide labeling services to our customers. In addition to labeling with methylene blue, we also offer a variety of types of labeling molecules to our customers. If you are interested in our services, feel free to contact us.

Reference

1. De Laet N, et al. Synthesis and evaluation of methylene blue oligonucleotide conjugates for DNA interstrand cross-linking[J]. Journal of Photochemistry and Photobiology A: Chemistry, 2016, 318: 64-70.