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Methylene Blue Labeling of Oligonucleotides

BOC Sciences' oligonucleotide modification services include a wide range of labeling options, providing customers with personalized solutions to meet their specific needs. With a wide range of options to choose from, we offer the right labeling service. We will help you select the appropriate labeling method and optimize the labeling method according to and for your specific needs.

Steps for labeling oligonucleotidesFig 1. Synthesis of methylene blue oligonucleotide conjugates. (De Laet et al., 2016)

What is Methylene Blue?

Methylene Blue belongs to the phenothiazine family of dyes and is a unique dye with many useful properties. Despite its high extinction coefficient in the visible region, it is weakly fluorescent due to its high intersystem crossing rate from the S1 excited state to the T1 triplet state. This property makes it an excellent photosensitizer and has been widely used for the production of highly reactive single linear oxygen species. Methylene blue-labeled oligonucleotides can be directly observed by common visualization methods such as gel electrophoresis. The methylene blue labeling method is applicable to various types of oligonucleotides, including DNA and RNA oligonucleotides, as well as oligonucleotides with modifying groups.

Methylene Blue Labeling Oligonucleotide Services

BOC Sciences can provide custom synthesis services for methylene blue labeled oligonucleotides. When designing an experiment, we need to select the length and type of oligonucleotide according to the customer's requirements, and then design a suitable experimental protocol. After completing the custom synthesis, the product needs to be characterized and validated for application to ensure that the product obtained is the target product. Throughout the process, strict quality control is required to ensure the consistency and high quality of the product.

Methylene Blue Labeled Oligonucleotide Service Process

Sample Requirements

BOC Sciences provides oligonucleotide labeling services to our customers. In addition to labeling with methylene blue, we also offer a variety of types of labeling molecules to our customers. If you are interested in our services, feel free to contact us.

Reference

  1. De Laet N, et al. Synthesis and evaluation of methylene blue oligonucleotide conjugates for DNA interstrand cross-linking[J]. Journal of Photochemistry and Photobiology A: Chemistry, 2016, 318: 64-70.
* Only for research. Not suitable for any diagnostic or therapeutic use.
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