Peptide-Oligonucleotide Conjugation

As a leading supplier of oligonucleotides, BOC Sciences is committed to providing our customers with comprehensive modification and labeling, offering affordable custom oligonucleotides or peptide-oligonucleotide conjugates.

Overview of POC

• What is POC

Peptide-oligonucleotide conjugates (POCs) consist of one or more residues of a linear or less commonly cyclic peptide linked by covalent bonds to an oligonucleotide or its analog. POC can improve cellular uptake, tissue delivery, bioavailability, and thus the overall efficiency of therapeutic nucleic acids such as antisense oligonucleotides and small interfering RNA.

Fig. 1 Schematic illustration of peptide-oligonucleotide conjugates (internal and terminal attachment). (Astakhova IK, 2013)

• How to Synthesize POC

1. Solid-phase stepwise synthesis
   It is the most straightforward method for POCs. In this method, both peptides and oligonucleotides are synthesized using an automated synthesizer.
2. Solid-phase fragment condensation
   Solid-phase fragment condensation (SPFC) refers to the synthesis of two oligomers on their respective solid carriers, with one of the oligomers still on the solid phase carrier when the affinity reaction is carried out. Linked options include:
1. Linked by amide
2. Linked by an oxime bond
3. Linked by phosphoramidate
4. Linked by acylurea bond
3. Solution phase synthesis
   The solution phase synthesis method requires peptides and oligonucleotides to be synthesized in two separate systems. After separation and purification, the two oligomers are then conjugated in the solution phase. Linked options include:
1. maleimide bond
2. Linking using oxime bonds and other related reactions
3. Thioether bond, disulfide bond
4. Using Diels-Alder reaction
4. Biological synthesis
   Synthesis of POCs under the catalysis of biocatalysts.

• Application of POC

1. Intracellular delivery
2. Increasing the stability of oligonucleotides
3. As an aptamer
4. Nanomaterials
5. Fluorescent probes
6. PCR precursors
7. Molecular markers
8. etc.

Introduction to BOC Sciences' POC Services

• What Are Our POC Services?

1. Synthesis of peptide sequences: BOC Sciences can synthesize peptide sequences according to the customer's requirements and needs.
2. Synthesis of oligonucleotide sequences: BOC Sciences can synthesize oligonucleotide sequences according to the customer's requirements and needs.
3. Preparation of peptide-oligonucleotide conjugates: BOC Sciences can couple the synthesized peptides and oligonucleotides, and purify and characterize the couples.
4. Optimization of conjugation reactions: BOC Sciences can improve the yield of peptide-oligonucleotide conjugation and the purity of conjugates by optimizing the reaction conditions.
5. Purification and analysis: BOC Sciences can purify and analyze peptide-oligonucleotide conjugates using a variety of techniques and methods, including HPLC, mass spectrometry, etc.
6. Provide customized services: BOC Sciences can provide customized peptide-oligonucleotide conjugation services to meet the specific needs and requirements of our customers.

• Why Choose Our POC Services

1. Extensive experience and technology: BOC Sciences has extensive experience and technology in peptide synthesis, oligonucleotide synthesis, chemical modification, purification and preparation.
2. Customized design: BOC Sciences provides customized design services according to customer needs and experimental purposes to ensure the best experimental results.
3. High purity products: BOC Sciences uses high quality raw materials and employs optimal reaction conditions and purification steps to ensure high purity and stability of products.
4. Professional technical support: BOC Sciences' scientists and technicians have the experience and knowledge to provide fast, professional and effective technical support to our customers.
5. Fast product delivery: BOC Sciences can provide customers with fast product delivery to meet the time requirements of experiments.
6. Competitive pricing: BOC Sciences offers reasonable and competitive pricing to its customers to ensure that they receive high quality service and products.