BOC RNA provides antisense oligonucleotides (ASOs) synthesis services according to your application to meet the research needs in biology, diagnostics, and drug discovery.
We provide many types of ASOs modifications, including
| Modification by type | Abbreviation | Features |
| 5-methylcytosine | 5-Me-dC ([5MedC] in sequence constructs) | Replacing dC with 5-methyl dC in the CpG motif will slightly increase the Tm of the antisense oligo. |
| 7-deaza-dG | Deaza-Dg | |
| Methyl RNA | 2'-OMe-RNA ([mA], [mC], [mG], & [mU] in sequence constructs) | Add modified bases in chimeric antisense designs, such as 2'-O-methoxyethyl (2'-MOE) to increase nuclease stability and affinity (Tm). |
| Phosphorothioate | PS (* in sequence constructs) | - Increase nuclease resistance.
- Higher solubility and nuclease stability
- Better membrane permeability
|
| LNA | Locked nucleic acid | - Lower immunostimulatory potential (compared to PS ASO)
- Shorter sequence
- Favorable binding affinity
- High affinity to the target sequence, which greatly improving mismatch discrimination
- Low overall toxicity
- Higher metabolic stability
|
What are antisense oligonucleotides (ASOs)?
Antisense oligonucleotides (ASOs) are oligonucleotides with 15-25 bases in length, designed to be antisense to the target RNA. The hybridization between ASOs and target RNA can mediate RNase H cleavage of RNA, thereby inhibiting the function of non-coding RNA (for example, miRNA, siRNA, piRNA, snoRNA, snRNA, exRNA, scaRNA, and lncRNA) or preventing mRNA translation.
Applications of ASOs
ASOs were first used more than two decades ago to suppress gene expression levels in vitro and in vivo. Antisense compounds have become useful tools for basic molecular biology, genomics, and proteomics research and are usually used for drug discovery, targeted screening, and verification.
Design considerations
- Hybridization site
mRNA folding into the secondary or tertiary structure is likely to prevent ASOs hybridization. Therefore, the unfolded mRNA region should be selected as the hybridization site.
Once the unfolded region is determined, it should be considered whether the region serves as a binding site for spliceosome, ribosome, protein, or other macromolecular assemblies, such as 5'cap, initiation codon, 3'untranslated region/polyA tail. Even if ASOs fail to activate RNase H, it can spatially block the mechanisms required for mRNA maturation or translation, which may lead to silencing.
- Nuclease degradation
In vivo and in vitro, nuclease activity quickly renders all-natural DNA ASOs useless. All ASOs need to be chemically modified to resist nuclease degradation to be effective.
Service process
Competitive advantages
- Flexible synthesis scales: 20nmol, 50 nmol, 100 nmol, 200 nmol, 1 µmol, 5 µmol, and 10 µmol. Bulk orders are available upon request.
- Experienced team: scientists and staff with over 15 years of experience in oligo synthesis and modifications.
- High purity: Chemical purification by PAGE or RP-HPLC (can be requested).
- High quality: QC analysis by MS, HPLC, or PAGE.
- Competitive prices.
- Fast delivery.
Contact us now to get technical advice and more information. Our technical support team will review your inquiries or provide a quotation as soon as possible!
