BOC RNA provides antisense oligonucleotides (ASOs) synthesis services according to your application to meet the research needs in biology, diagnostics, and drug discovery.
|Modification by type||Abbreviation||Features|
|5-methylcytosine||5-Me-dC ([5MedC] in sequence constructs)||Replacing dC with 5-methyl dC in the CpG motif will slightly increase the Tm of the antisense oligo.|
|Methyl RNA||2'-OMe-RNA ([mA], [mC], [mG], & [mU] in sequence constructs)||Add modified bases in chimeric antisense designs, such as 2'-O-methoxyethyl (2'-MOE) to increase nuclease stability and affinity (Tm).|
|Phosphorothioate||PS (* in sequence constructs)|
|LNA||Locked nucleic acid|
Antisense oligonucleotides (ASOs) are oligonucleotides with 15-25 bases in length, designed to be antisense to the target RNA. The hybridization between ASOs and target RNA can mediate RNase H cleavage of RNA, thereby inhibiting the function of non-coding RNA (for example, miRNA, siRNA, piRNA, snoRNA, snRNA, exRNA, scaRNA, and lncRNA) or preventing mRNA translation.
ASOs were first used more than two decades ago to suppress gene expression levels in vitro and in vivo. Antisense compounds have become useful tools for basic molecular biology, genomics, and proteomics research and are usually used for drug discovery, targeted screening, and verification.
mRNA folding into the secondary or tertiary structure is likely to prevent ASOs hybridization. Therefore, the unfolded mRNA region should be selected as the hybridization site.
Once the unfolded region is determined, it should be considered whether the region serves as a binding site for spliceosome, ribosome, protein, or other macromolecular assemblies, such as 5'cap, initiation codon, 3'untranslated region/polyA tail. Even if ASOs fail to activate RNase H, it can spatially block the mechanisms required for mRNA maturation or translation, which may lead to silencing.
In vivo and in vitro, nuclease activity quickly renders all-natural DNA ASOs useless. All ASOs need to be chemically modified to resist nuclease degradation to be effective.
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