BOC Sciences is an industry leader in high-quality oligonucleotide synthesis and formulation services. We provide professional custom ssRNA oligonucleotides with flexible synthesis scales to meet customers' diverse needs. All ssRNA sequences are produced using proprietary technology, providing industry-leading RNA quality.
Single-stranded RNA (ssRNA) is an RNA molecule containing only one strand. Unlike double-stranded DNA, most RNAs in organisms are single-stranded molecules. When they are folded into their secondary structures, RNA molecules can be present in both single-stranded and double-stranded domains. Although certain RNA viruses are double-stranded and composed of two complementary strands, it is generally presented in a viral form when it comes to ssRNA.
1. Positive-strand RNA virus (+ssRNA virus)
The ssRNA of positive-strand RNA viruses (e.g., poliovirus) can directly function as mRNA to translate early proteins, including RNA polymerase and regulatory proteins that inhibit host cell anabolism. Under the action of RNA polymerase, ssRNA (positive-strand) is used as a template for the complementary strand (negative-strand), thus creating the complemented double-strand, namely replication type. Moreover, new RNAs (positive-strand) are produced using the negative strand, which becomes replication intermediates. Nevertheless, new positive-strand RNAs can be used as mRNAs in synthesizing coating proteins.
2. Negative-strand RNA virus (-ssRNA virus)
Unlike positive-strand RNA viruses, negative-strand RNA viruses (e.g., orthomyxoviruses and paramyxoviruses) cannot be used as mRNAs. Furthermore, its complementary strand (positive-strand) must be synthesized as mRNA first and then translated into the protein molecule, producing a replicative form of nucleic acid that can be used as the template for daughter viral RNA. As for retroviruses (RVs), they reserved machinery of higher complexity, such as reverse transcriptase. Their reverse transcriptase genomic RNAs first synthesize proviral DNAs under the action of reverse transcriptase so that provirus can be integrated into host chromosomal DNA.
| Services | Custom ssRNA synthesis | |
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| Sequence Length | All ssRNAs are available from a few dozens up to several thousand bases | |
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| Purification | Desalted, deprotected, PAGE, HPLC, and/or MS | |
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Single-stranded RNA (ssRNA) consists of a single strand of nucleotides and is typically used in research to modulate gene expression and develop RNA-based assays. Unlike double-stranded RNA (dsRNA), which has two complementary strands, ssRNA can fold into secondary structures and is essential in processes like RNA interference and gene silencing.
ssRNA oligonucleotides are versatile tools used in a variety of applications including custom microRNA inhibitors, antisense oligos, ribozymes, aptamers, and RNA/DNA hybrids. They are also commonly used in RNA-based diagnostics, gene editing, FISH/ISH probes, and custom RNA duplexes for research purposes.
Custom ssRNA synthesis involves creating RNA sequences using chemical or enzymatic methods. The process includes selecting the right reverse transcriptase or RNA polymerase, choosing appropriate primers, and optimizing conditions to ensure high yield, sequence accuracy, and stability. We ensure that each sequence is synthesized to meet your specific research needs with high purity and precision.
We use rigorous quality control measures such as mass spectrometry (MS), HPLC, and PAGE to verify the purity and sequence identity of all synthesized ssRNA. Each batch is tested for copy number control and undergoes normalization to ensure consistent quality, and we provide a Certificate of Analysis (COA) for transparency and reliability.
BOC Sciences offers a wide range of modifications for ssRNA synthesis, including 2'-O-methyl RNA bases, backbone modifications, phosphorylations, amino modifiers, thiol modifiers, and spacers. These modifications are used to enhance the stability, functionality, and performance of ssRNA in your specific applications, such as RNA interference, gene expression modulation, or diagnostic assays.
We offer flexible scaling options for ssRNA synthesis, ranging from small-scale (0.05 µmol) to large-scale (1.0 µmol or more). Whether you need a small batch for preliminary research or a larger volume for extended studies, we can accommodate your specific project needs, ensuring high-quality results at any scale.
