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BOC Sciences is an industry leader in high-quality oligonucleotide synthesis and formulation services. We provide professional custom ssRNA oligonucleotides with flexible synthesis scales to meet customers' diverse needs. All ssRNA sequences are produced using proprietary technology, providing industry-leading RNA quality.
Single-stranded RNA (ssRNA) is an RNA molecule containing only one strand. Unlike double-stranded DNA, most RNAs in organisms are single-stranded molecules. When they are folded into their secondary structures, RNA molecules can be present in both single-stranded and double-stranded domains. Although certain RNA viruses are double-stranded and composed of two complementary strands, it is generally presented in a viral form when it comes to ssRNA.
1. Positive-strand RNA virus (+ssRNA virus)
The ssRNA of positive-strand RNA viruses (e.g., poliovirus) can directly function as mRNA to translate early proteins, including RNA polymerase and regulatory proteins that inhibit host cell anabolism. Under the action of RNA polymerase, ssRNA (positive-strand) is used as a template for the complementary strand (negative-strand), thus creating the complemented double-strand, namely replication type. Moreover, new RNAs (positive-strand) are produced using the negative strand, which becomes replication intermediates. Nevertheless, new positive-strand RNAs can be used as mRNAs in synthesizing coating proteins.
2. Negative-strand RNA virus (-ssRNA virus)
Unlike positive-strand RNA viruses, negative-strand RNA viruses (e.g., orthomyxoviruses and paramyxoviruses) cannot be used as mRNAs. Furthermore, its complementary strand (positive-strand) must be synthesized as mRNA first and then translated into the protein molecule, producing a replicative form of nucleic acid that can be used as the template for daughter viral RNA. As for retroviruses (RVs), they reserved machinery of higher complexity, such as reverse transcriptase. Their reverse transcriptase genomic RNAs first synthesize proviral DNAs under the action of reverse transcriptase so that provirus can be integrated into host chromosomal DNA.
| Services | Custom ssRNA synthesis | |
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| Sequence Length | All ssRNAs are available from a few dozens up to several thousand bases | |
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| Purification | Desalted, deprotected, PAGE, HPLC, and/or MS | |
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