Inquiry Basket
tRNA plays a crucial role in cellular translation in living organisms, and its expression and mutations have have been associated with certain diseases, including neuropathology and cancer development. It is important to monitor and determine changes in tRNA abundance and modification during biological processes. In recent years, quantification of tRNA gene expression has also received increasing attention due to its importance since it exert different roles depending on expression and post-transcriptional modification. BOC Sciences provides comprehensive tRNA expression analysis services to determine changes in tRNA expression by analyzing tRNA expression levels at the gene, codon, and amino acid levels.
Fig 1. Evaluation of differential tRNA gene expression. (Torres AG, 2019)
BOC Sciences provides a variety of services to quantify tRNA expression levels, including RT-qPCR, which accurately measures tRNA abundance to determine tRNA expression patterns; tRNA microarray, which achieves codon-level resolution by identifying tRNA anticodon loops thus determining gene expression of tRNAs; tRNA-sequencing methods, which provide rich data and information for identifying and validating differentially expressed tRNAs.
At BOC Sciences, we offer classic RT-qPCR services to quantify changes in the abundance of individual tRNAs to determine whether cells can selectively regulate protein expression by altering tRNA abundance.
Total RNA extraction, DNA digestion, first-strand cDNA synthesis, RNA digestion, Reverse transcription.
Multivariate analysis of tRNA expression pattern by employing t-SNE and K-means clustering methods.
Aiming to predict the regulation of gene expression, we introduce a microarray method based on fluorescent dye labeling technology to quantify tRNA in the samples. BOC Sciences provides tRNA microarray services to reliably quantify tRNA levels in human samples, helping to figure out the relationship between tRNA abundance and codon distribution. The probe used for our tRNA arrays are designed based on the genomic tRNA database and BOC Sciences’ own database. Here is our microarray-based tRNA analysis process:
BOC Sciences offers tRNA-seq service to generate full-length tRNA sequences with modification data for assessing differences in relative tRNA expression across treatments, tissues or subcellular fractions, our RNA-seq service has higher sensitivity to detect a wider range of expression.
Reference
Global Supplier of High-Quality RNA / DNA Products & Services.
