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Chemically modified siRNAs have better stability and potency compared to unmodified ones, and are important biological tools for studying gene functions and drug targets. BOC Sciences is committed to providing you with high quality and high purity phosphate backbone modified siRNAs, as well as phosphate backbone modified siRNAs according to your needs.
Phosphate Backbone Modifications in siRNA
Phosphate backbone modified siRNAs have been shown to significantly enhance the stability and in vivo bioavailability of siRNAs, mainly by modifying the phosphodiester moiety of ribonucleosides. The non-ester groups of siRNA phosphodiester bonds include -OH and =O, which can be substituted with sulfur, boron or acetate to give phosphorothioate, boron phosphate and phosphonoacetate bonds. Modification of the phosphate backbone of siRNA can alter the chemical structure of the nucleotide chain, thereby increasing the stability of siRNA, improving specificity, and optimizing the function of siRNA.
Figure 1. Phosphate backbone modifications of siRNA.
The modified phosphate backbone can protect the siRNA from being less susceptible to hydrolysis by nucleic acid exonucleases and nucleic acid endonucleases and becomes more stable. However, it is worth noting that some phosphate backbone modifications can affect RISC during RNA interference. For example, phosphorothioate can weaken the efficacy of siRNA by inhibiting the loading of siRNA into the RISC machine. Besides, it can also make the siRNA more immunogenic by making it more likely to bind to immune receptors. However, most of the phosphate backbone modifications contribute to the stability and gene silencing efficiency of siRNA.
Phosphate backbone-modified siRNA from BOC Sciences
| Modification | Quantity | Purification | Details | Price |
| 5'-Bromo-rU | 2 OD-250 OD | HPLC | A bromine atom is introduced at the uridine position at the 5' end of the RNA molecule, which is mainly used for cellular and tissue tracing. | Inquiry |
| 5'-Phosphate | 2 OD-250 OD | HPLC | The introduction of a phosphate group at the 5' end backbone of siRNA enables regulation of stability and affinity and further regulation of transcription and translation. | Inquiry |
| 5'-Amine | 2 OD-250 OD | HPLC | Modification by introducing an amine (NH2) group at the 5' end backbone of siRNA may be useful for subsequent bioconjugation. | Inquiry |
| 5'-Biotin | 2 OD-250 OD | HPLC | The modification of introducing biotin molecules into the 5' end backbone of siRNA can be used to track and localize the effects of RNAi. | Inquiry |
| 5'-Thiol | 2 OD-250 OD | HPLC | This modification increases the half-life of siRNA molecules in biological systems, resulting in more effective and sustained gene silencing. | Inquiry |
| 5'-Ferrocene | 2 OD-250 OD | HPLC | Attachment of a ferrocene group to the 5' end of an siRNA molecule enhances stability and is widely used for bioimaging, electrochemical detection, cell tracing and gene therapy. | Inquiry |
| 5'-Phosphorothioate | 2 OD-250 OD | HPLC | The replacement of one or more non-bridging oxygen atoms in the phosphate backbone of siRNA by sulfur atoms enhances the stability and extends the half-life of siRNA. | Inquiry |
| 5'-Phosphorodithioate | 2 OD-250 OD | HPLC | Phosphomethylation modification can increase the stability and intracellular stability of siRNA as well as improve the targeting effect. | Inquiry |
| 5'-Phosphoramidate | 2 OD-250 OD | HPLC | The introduction of alcohol ester group modification on the phosphate group in the phosphate backbone improves the stability of siRNA. | Inquiry |
| 5'-Phosphorothioate methyl ester | 2 OD-250 OD | HPLC | The introduction of methyl ester group on the sulfur atom of the phosphate backbone of siRNA can change the hydrophilicity and lipid solubility of siRNA and increase the intracellular stability. | Inquiry |
| 5'-Phosphocholine | 2 OD-250 OD | HPLC | The introduction of choline groups onto the phosphate backbone improves the stability and cell permeability of siRNAs and helps to enhance the biodistribution and transport of siRNAs. | Inquiry |
| 5'-Phosphoglycerol | 2 OD-250 OD | HPLC | Phosphoglycerol modification can alter the water solubility, stability and cell permeability of siRNA. | Inquiry |
| 5'-Phosphoethoxy | 2 OD-250 OD | HPLC | Replacement of one or more phosphate groups on the phosphate backbone using vinyl groups can alter the physical and chemical properties, stability and affinity of siRNA. | Inquiry |
BOC Sciences has a team of experts with a decade of experience in RNA synthesis and state-of-the-art facilities to ensure high quality and competitive prices for phosphate backbone-modified siRNA. For more custom phosphate backbone-modified siRNA, please feel free to contact us.
* Only for research. Not suitable for any diagnostic or therapeutic use.
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