BOC Sciences provides high-quality, cost-effective custom RNA Oligos synthesis services with flexible synthesis specifications to meet the diverse needs of a wide range of researchers.
RNA activation is a type of RNA regulation that activates the expression of target genes at the transcriptional level. Small molecule RNAs that can activate gene transcription are known as small activating RNAs (saRNAs). saRNAs are short, double-stranded oligonucleotides, usually consisting of 19 to 30 nucleotides, with a specific sequence and structure. Compared with traditional gene therapy, saRNAs have unparalleled advantages in tumor therapy, and have broad application prospects in both tumor pathogenesis and tumor research.
Similar to other RNAs (antisense oligonucleotide/ASO, RNAi, siRNA, etc.), saRNAs work by binding to target nucleic acids, but unlike other RNAs, saRNAs ultimately activate gene expression.
The mechanism of saRNA action is that the double-stranded saRNA enters the cell by endocytosis and is then loaded onto the AGO2 protein. After the carrier chain is degraded, AGO2 and the active chain complex are transported into the nucleus and bind to the promoter region. Subsequently, it attracts RNA polymerase by binding to RNA deconjugating enzyme and RNA polymerase linker protein (CTR9) to activate the transcription of the corresponding genes, and ultimately promotes the expression of the corresponding proteins.
Fig 1. Proposed model of small activating RNA mechanism. (Kwok et al., 2019)
BOC Sciences has developed a powerful bioinformatics platform to design saRNAs for target gene activation. These saRNA sequences recognize elements upstream of the mRNA transcription start site and downstream of the polyA site.
Currently, it is difficult to screen for suitable saRNA target sites, and multiple saRNA molecules usually need to be designed for screening. saRNA design and screening methods need to be further optimized to improve the efficiency of RNAa. BOC Sciences needs to consider the following factors when performing saRNA design. Selection of the positive-sense DNA sequence as the template and the complementary RNA single-stranded sequence as the guide strand.
The custom synthesis service of saRNA is similar to that of dsRNA, which requires the setting of sequence length, purity, and modification according to the requirements, and then the products are prepared under strict quality control. Customers can directly provide us with the designed sequences or let us design them according to the requirements. Our technical team is able to provide you with customized synthesis of small-scale, medium-scale and large-scale saRNA.
The saRNA chemical modification services offered by BOC Sciences are important for the stability of small molecule nucleic acid sequences in the physiological environment. The choice of motifs and sites for chemical modification needs to be based on the principle of safety first and ensure that saRNA activation remains unchanged. Although chemical moiety modification does not solve the problem of precise delivery of nucleic acid sequences, this in vitro experiment validates the use of chemical modification to improve saRNA sequence stability in a human physiological environment.
The synthesized products need to be strictly purified by HPLC, avoiding RNase contamination in the whole process, and providing you with free HPLC test reports.
We provide various types of Oligo synthesis services, such as RNA Oligo/DNA Oligo synthesis and modification.
BOC Sciences provides you with the most considerate service at the most favorable price.
BOC Sciences' advanced laboratory equipment and perfect technical support allow you to get the most professional service in the shortest time.
Small activating RNA (saRNA) is a short, double-stranded RNA molecule that activates gene expression at the transcriptional level. It works by binding to specific target regions in the genome and promoting RNA polymerase activity, leading to the expression of the target gene.
saRNA design involves selecting target sites in the promoter regions of genes. The design process requires careful consideration of the base-pairing of the RNA to the DNA, avoiding regions with high GC content, and ensuring the 3' end of the RNA is thermodynamically stable for effective gene activation.
The primary challenge is identifying suitable target sequences for gene activation. saRNA design often requires screening multiple sequences to find the most effective targets that minimize off-target effects and maximize activation efficiency.
BOC Sciences offers custom saRNA synthesis services, including design, production, and optimization. Our bioinformatics platform ensures the best possible sequences for gene activation, and we can synthesize small, medium, or large-scale saRNAs based on your requirements.
We offer a variety of chemical modifications for saRNA, such as phosphorothioate modifications, fluorophore labeling, and stabilizing chemical groups. These modifications help enhance the stability of saRNA in physiological environments and improve its delivery efficiency.
Our saRNA products undergo rigorous purification through HPLC to ensure they are free from RNase contamination. We also provide HPLC test reports and other quality checks to confirm the accuracy and purity of the synthesized saRNAs.
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