Long RNA transcription synthesis is a key process in many applications in biotechnology and medicine, such as gene therapy, RNA interference and aptamer development. However, the synthesis of long RNA transcripts remains challenging due to the complexity of synthesizing target RNA sequences with high fidelity and yield, and BOC Sciences offers a comprehensive range of services for the preparation of long RNA transcripts, including solid-phase synthesis, enzymatic synthesis, and chemical synthesis.
BOC Sciences offers Long RNA transcription synthesis services for design and gene synthesis using plasmids containing phage promoters such as T7, T3, or SP6. Long RNA transcription synthesis from phage can produce high-quality RNA thousands of nucleotides long, and RNA modifications such as fluorescent labeling, end-capping, or modified RNA bases can also be performed. In addition, we offer the following synthesis services.
Our solid phase synthesis service can prepare RNA transcripts up to 300 nt in length. Solid phase synthesis involves attaching the 5' end of the RNA to a solid phase support and then sequentially extending the RNA strand by adding nucleotide monomers. This method allows long RNA transcripts to be prepared with high precision and purity.
Our enzymatic synthesis services can be used to prepare RNA transcripts with modified nucleotides, which can be used for a variety of applications. Enzymatic synthesis involves the use of RNA polymerases to synthesize RNA transcripts by doping with nucleotides triphosphate. This method allows the incorporation of modified nucleotides into the RNA, which can enhance the stability and specificity of the RNA molecule.
We also offer chemical synthesis of RNA transcripts, which involves chemical coupling of nucleotide monomers to form RNA strands. This method can be used to prepare modified RNA transcripts with unnatural nucleotides or modified linkages. Chemical synthesis can also be used to prepare RNA transcripts with site-specific modifications, such as fluorescent or biotinylated labeling.
The synthesis of long RNA transcription provides a variety of synthetic scales with yields ranging from micrograms to milligrams. Our quoted prices for RNA transcripts are based on the starting synthesis scale. This is due to the high degree of variability that exists between templates. These variations are affected by sequence, length and base composition. The incorporation of modifying bases can also affect the final yield. Some modifications (e.g., 5-methyl-C) have little effect on the final yield, while 2' fluorine modifications can have a large effect.
All RNA transcripts are produced under a strict quality management system, from reagent acquisition from qualified suppliers to oligonucleotide to DNA template production. Each step is screened by mass spectrometry, agarose size check and bidirectional DNA sequencing at multiple locations. After transcription, the RNA is scrutinized using denaturing PAGE electrophoresis, real-time PCR, and other additional custom assay development as requested by the customer.
At BOC Sciences, we are committed to quality and customer satisfaction. Our team of experts works closely with each client to understand their specific requirements and provide customized solutions that meet their needs. Our expertise in the use of modified nucleotides, chemical synthesis and purification methods enables us to provide tailored solutions for each project.
Contact us for more about your needs. The expertise of our scientists, engineers and support team are at your disposal.
Long RNA transcription synthesis refers to the process of creating RNA molecules of significant length (typically greater than 100 nucleotides) through methods like solid-phase synthesis, enzymatic synthesis, or chemical synthesis. It is essential for various applications such as gene expression studies, RNA interference, and aptamer development.
BOC Sciences offers three primary methods for long RNA transcription synthesis: solid-phase synthesis, enzymatic synthesis, and chemical synthesis. These methods allow for the production of high-quality RNA transcripts with specific modifications for diverse applications.
Solid-phase synthesis involves attaching the RNA to a solid support and adding nucleotide monomers in a sequential manner, while enzymatic synthesis uses RNA polymerases to extend the RNA strand with nucleotide triphosphates. Enzymatic synthesis allows for the incorporation of modified nucleotides, improving stability and specificity.
Yes, BOC Sciences offers RNA modification services during synthesis, including the incorporation of modified bases such as fluorescent labels, biotinylation, or end-capping. These modifications are useful for enhancing RNA stability, cellular uptake, and detection sensitivity.
The yield of RNA transcription synthesis can be influenced by factors such as the length of the RNA, the base composition, and the presence of modified nucleotides. Some modifications, like 2' fluorine modifications, can significantly impact the final yield, while others, like 5-methyl-C, have a lesser effect.
BOC Sciences provides RNA synthesis services on various scales, ranging from micrograms to milligrams of RNA transcript. The scale of synthesis is tailored to the customer's needs, with pricing based on the starting synthesis scale and template variations.
