Plate oligonucleotide synthesis is designed for projects that need many defined sequences delivered in an organized, automation-compatible format. Compared with ordering individual tubes, plate production places greater emphasis on sequence-to-well mapping, cross-sample consistency, normalization strategy, plate layout, and downstream liquid-handling compatibility. Our plate oligonucleotide synthesis service supports DNA and RNA sequence sets for PCR, qPCR, NGS, screening, cloning, probe development, RNAi research, and other high-throughput molecular workflows.
We support 96-well and 384-well project formats with options for single-stranded oligos, primer pairs or mixes, duplexed constructs, selected modifications, purification, dry or resuspended delivery, and project-specific QC. Each plate project is reviewed as a complete sequence set so that scale, sequence length, modification load, well assignment, concentration targets, and assay requirements can be managed consistently.
Plate Mapping: A single well-position error can propagate through an entire screening experiment. We use a defined sequence-to-well map and naming convention so that sample identity remains traceable from order intake through delivery.
Sequence Diversity: Large projects may combine different lengths, GC content, degenerate positions, or modification types. We review the sequence distribution and can recommend subgroups when one processing condition is unlikely to fit the full plate.
Yield & Normalization: Raw synthesis yield varies by sequence and chemistry. Projects that require comparable working concentrations may need post-synthesis quantitation, normalization, or controlled resuspension rather than simple dry delivery.
Purification Throughput: High-resolution purification can improve sequence quality but may not be necessary for every plate workflow. We match desalting, cartridge-style cleanup, HPLC, PAGE, or other available options to sequence length and experimental sensitivity.
Automation Compatibility: Plate type, working volume, sealing, orientation, and final concentration affect downstream robotics. We align delivery specifications with the customer's liquid handler or assay workflow when those requirements are provided.
Our plate service is intended for teams that need organized sequence sets rather than isolated custom oligos. DNA and RNA projects can be configured around sequence count, plate density, synthesis scale, modification strategy, purification, QC, and final presentation.
For projects that include specialized constructs, we can coordinate with custom oligo synthesis, custom ssDNA oligo synthesis, or RNA-specific workflows as appropriate.
| Service Options | Product | Synthesis Scale | Length | Price |
| Custom DNA Plate Oligo | Single-stranded DNA Plate Oligo | 10 nmol | 15 - 60 Bases | Inquiry |
| 25 nmol | 15 - 60 Bases | Inquiry | ||
| 50 nmol | 10 - 90 Bases | Inquiry | ||
| 100 nmol | 10 - 90 Bases | Inquiry | ||
| Primer mix DNA Plate Oligo | 10 nmol | 15 - 60 Bases | Inquiry | |
| 25 nmol | 15 - 60 Bases | Inquiry | ||
| 50 nmol | 10 - 90 Bases | Inquiry | ||
| 100 nmol | 10 - 90 Bases | Inquiry | ||
| Duplexed DNA Plate Oligo | 50 nmol | 10 - 90 Bases | Inquiry | |
| 100 nmol | 10 - 90 Bases | Inquiry | ||
| 250 nmol | 5 - 100 Bases | Inquiry | ||
| Custom RNA Plate Oligo | Single-stranded RNA Plate Oligo | 10 nmol | 5-60 bases | Inquiry |
| 25 nmol | 5-60 bases | Inquiry | ||
| 50 nmol | 5-60 bases | Inquiry |
Plate configuration should be chosen from the downstream workflow backward. Sequence type, well density, pairing requirements, and delivery format all affect how easily the plate can be used in an automated or manual assay.
| Plate Configuration | Typical Content | Key Planning Variables | Delivery Options | Best-Fit Workflows |
| 96-well ssDNA plate | One DNA oligo per occupied well | Sequence count, length distribution, scale, modifications | Dry or resuspended; optional normalization | PCR/qPCR, cloning, sequencing, screening |
| 384-well ssDNA plate | High-density DNA sequence sets | Automation compatibility, low working volume, plate map accuracy | Dry or resuspended; automation-oriented formatting | Large assay panels, screening libraries, NGS support |
| Primer-pair plate | Forward/reverse primers arranged by assay | Pairing scheme, Tm balance, orientation, concentration | Separate wells or project-defined paired layout | PCR and qPCR assay setup |
| Duplex DNA plate | Annealed complementary DNA pairs | Strand pairing, stoichiometry, annealing, duplex identity | Pre-annealed duplexes with defined well map | Hybridization assays, controls, screening constructs |
| RNA plate | Single-stranded or paired RNA sequences | RNase control, modification pattern, sequence length | Dry or resuspended; optional duplexing | RNAi research, RNA probes, antisense screening |
| Modified oligo plate | DNA or RNA with selected functional groups | Modification compatibility, purification burden, well grouping | Project-specific format and QC | Probe panels, capture assays, conjugation-ready libraries |
A complete sequence file and plate map are the most important starting materials. The following information helps prevent avoidable reformatting and downstream handling problems.
| Project Input | What to Provide | Why It Matters | Common Risk if Missing | Affected Service Step |
| Sequence list | 5′→3′ sequence for every oligo | Defines chemistry and sample identity | Transcription or mapping errors | Design review and synthesis |
| Plate map | Well ID matched to sequence/sample name | Maintains traceability | Well-position confusion | Formatting and delivery |
| Oligo type | DNA, RNA, primer pair, duplex, mixed project | Determines synthesis and handling route | Incompatible processing assumptions | Production planning |
| Modification map | Exact modification and position for each sequence | Controls chemistry and purification | Incorrect functional group or attachment site | Synthesis and QC |
| Final concentration/volume | Requested resuspension target or dry delivery | Aligns plate with downstream automation | Extra manual normalization | Post-synthesis formatting |
| QC expectation | Representative, per-oligo, or other agreed plan | Balances analytical depth and project scale | Under- or over-testing | Release documentation |
The workflow emphasizes data integrity and plate-level consistency in addition to oligonucleotide chemistry.
Import the sequence list, confirm naming conventions, detect formatting inconsistencies, and identify unusual lengths, degenerate bases, or modification requests.
Finalize 96- or 384-well layout, oligo type, synthesis scale, purification level, QC plan, and dry versus resuspended delivery.
Produce the defined oligonucleotide set using chemistry appropriate for the DNA, RNA, and modification requirements in each project group.
Apply desalting or higher-resolution purification according to the agreed specification and the needs of the sequence set.
Quantify and format the oligos into the approved plate map, with optional resuspension, normalization, pairing, or annealing.
Complete the agreed analytical checks and deliver the plate map, sample identifiers, QC documentation, and sealed plates in the specified format.
High-throughput oligo programs benefit when sequence data, chemistry, and plate logistics are managed as one project.
Plate delivery is useful whenever many related oligonucleotides must be tracked, transferred, and tested in parallel.
For a plate oligo quote, provide the sequence list, plate format, preferred well map, DNA or RNA type, desired scale, modifications, purification level, QC expectations, and dry or resuspended delivery requirements. If the plate will feed an automated workflow, include the target concentration, working volume, and plate constraints so the final format can be planned accordingly. Contact us to discuss a 96-well or 384-well oligonucleotide synthesis project.
Provide a sequence list, sample names, well positions or preferred plate map, DNA or RNA type, scale, modifications, purification level, QC expectations, and dry or resuspended delivery requirements.
Yes, plate format can be selected according to sequence count, liquid-handling workflow, working volume, and downstream assay requirements.
Yes, concentration normalization or controlled resuspension can be included when requested. This is useful when the plate will feed screening or automated assay workflows.
Selected labels, affinity tags, phosphorylation, reactive handles, spacers, backbone or sugar modifications may be incorporated after chemistry and plate-processing feasibility review.
QC can be configured as representative, per-oligo, or another project-defined testing plan. The appropriate depth depends on sequence count, chemistry complexity, downstream sensitivity, and budget.
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