Custom FISH Probe Service

BOC Sciences offers custom FISH probe design and manufacturing services, from simple modifications to comprehensive novel designs. Our expert team is capable of designing probes that fulfill the specific needs of our customers.

Simply contact us to discuss your specific requirements with our experts.

What is FISH

FISH (Fluorescence In Situ Hybridization) is an oligonucleotide probe using direct fluorescent group labeling or indirect labeling, such as biotin. FISH provides a method for visualizing and mapping genetic material in individual cells to validate chromosomal abnormalities and other genetic mutations.

How does FISH work?

FISH is a macromolecular recognition technique based on the complementary nature of DNA or DNA/RNA double strands. Its basic principles are shown below. (a) DNA probe and target sequence are the basic elements of FISH. (b) Prior to hybridization, DNA probe is labeled using PCR, cut-and-pan, and labeled with random primer. Two labeling strategies, indirect labeling (left panel) and direct labeling (right panel) are commonly used. Modified nucleotide-labeled probes containing semi-antigens are used for indirect labeling, while direct labeling adapts directly modified nucleotides containing fluorophores. (c-d) Both labeled probes and target DNA apply the denatured-annealed-denatured process to hybridize target DNA and nucleic acid probe. (e) If probe is indirectly labeled, an additional step is required to visualize the non-fluorescent semi-antigen with an enzymatic or immunological detection system. The hybrid between target gene and probe is formed according to the principle of base complementarity, and target signal is visualized by fluorescence microscopy. This allows for the determination of relative qualitative, quantitative and localization analysis of the nucleic acid sequence.

The principle of fluorescence in situ hybridization (FISH). Fig.1 The principle of fluorescence in situ hybridization (FISH). (Michael R., 2005)

What is the application of Fish?

  • Experimental techniques for qualitative, localized, or quantitative analysis of DNA or RNA at the level of tissues, cells, chromosomes, etc.
  • Applications in scientific research and disease diagnosis studies.
  • Comparison of gene chromosome arrangement between related species.

Benefits of BOC Sciences' FISH probes

  • Safe, fast, and sensitive; the probe pertains longer production life.
  • Small molecular weight and low sequence complexity minimize hybridization time by 10 times compared to cloning probes.
  • Oligonucleotide probe sequence length optional range is flexible can meet a variety of gene form detection.
  • Direct labeling of multiple motif tags, minimizes synthesis time, and competitive prices for massive synthesis.
  • Capable of multiple gene form detection analysis, capable of performing temporal, spatial and quantitative multi-dimensional analysis.
  • Particular oligonucleotide probes can be designed by screening sequences and/or selecting relatively long sequences.
  • Can be applied to fresh, frozen, or paraffin-embedded specimens, as well as punctures, exfoliated cells and many other materials.


  1. Michael R.; et al. The New Cytogenetics: Blurring The Boundaries With Molecular Biology. Nature Reviews Genetics. (2005): 782-792.
  2. Cui C; et al. Fluorescence In situ Hybridization: Cell-Based Genetic Diagnostic and Research Applications. Frontiers in Cell and Developmental Biology. 2016, 4.
* Only for research. Not suitable for any diagnostic or therapeutic use.
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