Oligonucleotides have been modified to provide richer and superior properties for a wider range of applications. BOC Sciences offers labeling of oligonucleotides for a variety of molecules, such as cholesterol, tocopherol, acridine, etc.
Acridine-labeled oligonucleotides are molecular probes commonly used in biological experiments. Acridine-labeled oligonucleotides can be used to detect the presence and localization of target nucleic acid sequences when performing nucleic acid hybridization experiments. In addition, acridine-labeled oligonucleotides can be used in genomics research, gene expression regulation research, DNA sequencing, molecular diagnosis and other fields. By labeling specific oligonucleotide sequences as acridine-tagged oligonucleotides, recognition and detection of target sequences can be achieved.
Based on its rich labeling technology and experience, BOC Sciences can provide multiple ways of acridine labeling of oligonucleotides and apply them in different fields.
BOC Sciences can design acridine-labeled oligonucleotides from various aspects, which are mainly divided into the following.
Identify the target sequences that the customer wants to probe or analyze, which can be specific gene fragments, RNA sequences, or other nucleic acid sequences of interest.
Select the location where the acridine marker is to be introduced on the oligonucleotide. The acridine marker is usually placed at the end of the oligonucleotide or at an internal location.
Common methods include the introduction of a modifying moiety with a fluorophore during chemical synthesis, or subsequent modification reactions on already synthesized oligonucleotides.
The length of the oligonucleotide should be long enough to ensure specific binding to the target sequence, but it is also important to avoid excessively long lengths that result in reduced hybridization efficiency. Sequences should be chosen to be complementary to the target sequence and avoid their own secondary structure or repetitive sequences.
The modifying group of the marker should have good stability, which can maintain the integrity and activity of the marker under the experimental conditions. In addition, the labeled oligonucleotide should have sufficient specificity to ensure specific binding to the target sequence.
In the process of synthesizing an oligonucleotide, a modifying group with an acridine label is introduced at the end. This process can be achieved by reacting between the labeled modifying group and the nucleotide unit using a suitable chemical coupling reagent.
BOC Sciences offers internally labeled oligonucleotides in which an acridine tag is introduced into an internal position of the oligonucleotide. Internally labeled oligonucleotides can be used for a variety of applications such as hybridization and probe design. For internally labeled oligonucleotides, it is necessary to choose the appropriate position and modification strategy to ensure the stability and specificity of the label. Internal labeling is more flexible than end-labeled oligonucleotides, suitable for qualitative and quantitative analysis, and can be applied to a variety of experimental techniques.
BOC Sciences is committed to providing comprehensive customized acridine-labeled oligonucleotides services within tight deadlines, at reasonable prices, and with stringent quality control of our products. If you are interested in our services, feel free to contact us .
Acridine-labeled oligonucleotides are used as molecular probes to detect and localize specific nucleic acid sequences in applications like nucleic acid hybridization. They are widely used in genomics research, DNA sequencing, and gene expression analysis.
Acridine labeling involves covalently attaching the acridine molecule to an oligonucleotide, either at the end or internally, to enable detection and analysis. This labeled oligonucleotide can then bind to complementary target sequences, providing insight into gene activity or sequence identification.
Yes, BOC Sciences offers custom design services for acridine-labeled oligonucleotides, including selecting the target sequence, labeling position, and synthesis method. We ensure the final product meets your specific experimental needs with high specificity and stability.
End-labeled oligonucleotides have the acridine tag attached at one end, while internally labeled oligonucleotides have the tag positioned at an internal location. Internally labeled oligonucleotides offer more flexibility, making them suitable for quantitative and qualitative analysis, while end-labeled versions are ideal for simpler applications.
Yes, acridine-labeled oligonucleotides can be combined with other detection methods like fluorescence and gel electrophoresis. Their ability to produce a detectable signal allows integration into multi-modal research applications for comprehensive analysis.
