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With the increasing demand and application of oligonucleotide-based diagnostic probes in biomedical research and industrial production, BOC Sciences looks to the global market to provide you with customization and development of dual-labeled probes. Your various dual-labeled probes project needs, such as various modifications or labeling at the 5' end, 3' end, and specified positions are all available at BOC Sciences.
Dual-labeled probes, also known as hydrolysis probes, are 20-30 bp DNA or RNA oligonucleotides that are labeled with two different dye groups at the 5' and 3' ends of the probe. Usually the reporter group is located at the 5' end of the dual-labeled probe and the quenching group is located at the 3' end of the dual-labeled probe.
Figure.1 The sturcture of dual labeled probes.
Dual-labeled probes have the advantages of high sensitivity and high specificity, thus realizing accurate quantification in the fields of pathogen detection, gene polymorphism research, and gene mutation detection. Moreover, the features of dual-labeled probes make it possible to perform multiplexed assays to detect multiple target sequences simultaneously.
BOC Sciences has continuously optimized oligonucleotide synthesis and modification technologies and procedures to provide customized dual-labeled probes for your single or multiplexed real-time PCR applications. We offer a wide selection of fluorescent probes, or you can inform us of the labeling site and submit the dye for modification, leaving us to complete the dual-labeled probe preparation.
Figure 2. The FRET principle for selection of quencher and reporter.
| ATTO-390(ex: 390 nm, em: 476 nm) | ATTO-425(ex: 439nm, em: 485 nm) | LCCyan500(ex: 439nm, em: 485 nm) |
| 6-FAM(ex: 495 nm, em: 520 nm) | Fluo(ex: 495 nm, em: 520 nm) | FITC(ex: 490 nm, em: 525 nm) |
| ATTO-495(ex: 498 nm, em: 526 nm) | TET(ex: 521 nm, em: 536 nm) | ATT0-520(ex: 517nm, em: 538nm) |
| JOE(ex: 522 nm, em: 548 nm) | Yakima Yellow(ex: 530 nm, em: 549 nm) | HEX(ex: 535 nm, em: 556 nm) |
| ATTO-Rho6G(ex: 533 nm, em: 557 nm) | Cy3(ex: 546 nm, em: 563 nm) | TAMRA(ex: 564 nm, em: 579 nm) |
| ROX(ex: 576 nm, em: 601 nm) | Texas Red(ex: 586 nm, em: 610 nm) | LCRed610(ex: 590 nm, em: 610 nm) |
| ATTO-Rho13(ex: 603 nm, em: 627 nm) | DY480XL(ex: 500 nm, em: 630 nm) | ATTO-Rho14(ex: 625 nm, em: 646 nm) |
| LCRed640(ex: 625 nm, em: 640 nm) | Cy5.5(ex: 683 nm, em:705 nm) | IRD700(ex: 685 nm, em: 705 nm) |
If you have any other oligonucleotide modification requirements, please feel free to contact us.
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