MicroRNAs (miRNAs) are small, non-coding RNA molecules that have emerged as central regulators in gene expression, impacting a vast array of biological processes such as cell proliferation, differentiation, apoptosis, and metabolism. In recent years, the role of miRNAs has been increasingly elucidated in areas such as developmental biology, oncology, neurobiology, and immune regulation—making them indispensable in functional genomics and disease modeling. At BOC Sciences, we leverage over two decades of biochemical expertise to offer custom miRNA synthesis services with unparalleled accuracy, reproducibility, and biological relevance. Our platform is engineered to deliver synthetic miRNA molecules tailored to your experimental requirements with full customization and stringent quality control, empowering advanced research in gene silencing, pathway elucidation, and functional validation studies.
The synthesis of miRNAs demands precision, as their biological efficacy is highly dependent on sequence fidelity, structural integrity, and chemical modifications. Our proprietary platform uses solid-phase oligonucleotide synthesis (SPOS) combined with high-efficiency purification systems (such as HPLC or PAGE) to deliver synthetic miRNAs with high purity.
Key parameters we control during synthesis include:
This meticulous process ensures functional activity and in vivo-like mimicry of endogenous miRNA behavior.
At BOC Sciences, we recognize the diverse and evolving needs of researchers working with miRNAs. To meet these demands, we provide a full spectrum of tailored miRNA synthesis services designed to support a variety of experimental objectives and biological models. Our offerings encompass chemically synthesized oligonucleotides, advanced chemical modifications, functional mimics and inhibitors, as well as vector-based delivery systems, enabling precise modulation of miRNA activity with optimal stability and specificity.
miRNA mimics are synthetic double-stranded RNA molecules engineered to replicate endogenous mature miRNAs, enabling functional overexpression in cells. Our miRNA mimics are produced with precision strand design to ensure:
This service is ideal for gene knockdown validation, pathway analysis, and high-throughput screening in diverse cell types, including primary cells and stem cells.
miRNA inhibitors (also known as antimiRs) are chemically modified single-stranded antisense oligonucleotides designed to bind and neutralize endogenous miRNAs, effectively silencing their regulatory function. Key features include:
This service facilitates loss-of-function studies, helping elucidate the biological role of specific miRNAs in gene regulation and disease models.
Agomirs are chemically enhanced miRNA mimics modified for improved cellular uptake and in vivo stability, commonly conjugated with cholesterol or other lipophilic molecules. Our agomir synthesis service features:
Agomirs are widely used for systemic delivery in preclinical studies, enabling efficient modulation of miRNA pathways in whole organisms.
Antagomirs are specialized miRNA inhibitors chemically modified for systemic in vivo administration. Key characteristics of our antagomirs include:
This service supports functional inhibition of miRNAs in vivo, aiding therapeutic target validation and mechanistic research.
miRNA sponges are engineered transcripts containing multiple tandem binding sites complementary to target miRNAs, designed to competitively inhibit endogenous miRNA activity. Our miRNA sponge services include:
Sponges enable durable and specific miRNA inhibition, providing a valuable tool for dissecting miRNA regulatory networks.
For applications requiring stable, long-term miRNA modulation, we provide lentiviral packaging of custom miRNA constructs. Our service encompasses:
This approach facilitates miRNA overexpression or inhibition in hard-to-transfect cells, including primary and stem cells, supporting advanced gene function studies and phenotypic screening.
Each of these custom miRNA synthesis and delivery options is supported by BOC Sciences' commitment to scientific rigor, reproducibility, and seamless integration into your research pipeline. Our diverse offerings ensure that you have the optimal toolset for comprehensive miRNA functional analysis.
At BOC Sciences, our miRNA synthesis services follow a robust, highly controlled workflow designed to ensure maximum product quality, bioactivity, and consistency. Each stage is executed with precision and stringent quality control to meet the demanding requirements of advanced research applications.
This systematic approach guarantees that every miRNA product delivered by BOC Sciences is scientifically robust and optimized for your experimental success.
Selecting the right partner for miRNA synthesis is critical for obtaining reliable, reproducible results that accelerate your research. BOC Sciences stands out in the industry by delivering unparalleled quality, customization, and scientific expertise. The following core advantages demonstrate why our miRNA synthesis services are the optimal choice for your preclinical research needs:
We customize miRNA sequences with precise chemical modifications to enhance stability, specificity, and biological activity, minimizing off-target effects.
Using cutting-edge solid-phase synthesis combined with HPLC and PAGE purification, we deliver high-purity miRNA products with excellent sequence fidelity.
Every batch undergoes mass spectrometry, UV quantification, and functional validation. Endotoxin testing ensures safety for cell-based studies.
From mimics and inhibitors to lentiviral packaging, our full-service portfolio supports diverse research needs under one roof.
With 20+ years' experience, our team provides tailored guidance throughout your project to maximize results.
Efficient workflows and cold-chain shipping ensure timely arrival and product integrity.
Choosing BOC Sciences' miRNA Synthesis Services means entrusting your research to a partner who combines scientific rigor, technical excellence, and personalized service. Our commitment is to empower your discovery process with reliable miRNA tools that drive innovation and precision in gene regulation studies.
Synthetic microRNAs (miRNAs) have transformed molecular biology and preclinical research by providing a precise mechanism to modulate gene expression at the post-transcriptional level. The custom miRNA synthesis services offered by BOC Sciences support a wide range of sophisticated applications, enabling researchers to delve deeply into gene regulation, disease mechanisms, and therapeutic target validation.
Synthetic miRNA mimics and inhibitors are essential for detailed functional characterization of miRNAs and their target genes. Through gain-of-function and loss-of-function experiments, researchers can dissect the complex roles miRNAs play in cellular physiology and pathology. This includes:
Example: Introduction of synthetic miR-21 mimics into cancer cells leads to activation of oncogenic pathways, facilitating understanding of tumor progression, whereas application of anti-miR-21 inhibitors reverses malignant phenotypes, thereby elucidating the critical function of miR-21 in tumor biology.
Synthetic miRNAs enable precise interrogation of candidate genes identified in drug discovery pipelines. By selectively enhancing or silencing gene expression post-transcriptionally, miRNA reagents provide a robust platform to:
Use Case: In disease-relevant models, transfection of anti-miR inhibitors targeting a specific miRNA involved in pathogenesis can demonstrate phenotypic rescue or exacerbation, thereby validating the target's therapeutic potential and guiding lead compound optimization.
miRNAs possess several features—high stability in biofluids, tissue specificity, and disease association—that make them excellent biomarkers. Synthetic miRNAs serve critical roles in:
Example: Synthetic miR-155 can be used as a standardized control to confirm elevated expression in inflammatory or autoimmune disease models, enabling robust validation of biomarker candidates.
While clinical development is outside the scope of our services, BOC Sciences plays a pivotal role in supporting preclinical therapeutic research by providing chemically optimized miRNA reagents tailored to enhance stability, specificity, and delivery efficiency. These include:
Such reagents underpin proof-of-concept studies, dose-response optimization, and safety profiling necessary to advance miRNA therapeutics from bench to preclinical validation.
Synthetic miRNAs facilitate sophisticated interrogation of cellular signaling pathways by enabling targeted modulation of nodal regulatory miRNAs within complex networks. This application includes:
Use Case: Co-transfection of miR-34a mimics alongside inhibitors targeting oncogenic pathways can reveal critical dependencies and potential combination therapies in cancer models.
miRNA sponges are engineered constructs containing multiple tandem binding sites complementary to a miRNA of interest. These molecules act as competitive inhibitors by sequestering endogenous miRNAs and preventing them from binding to their natural targets. Applications include:
This approach is particularly powerful for unraveling complex regulatory networks and identifying indirect effects of miRNA modulation.
Synthetic miRNAs also play a critical role in toxicological assessment by helping researchers:
The precision and versatility of synthetic miRNAs make them ideal components in emerging synthetic biology applications, including:
Integration of synthetic miRNAs into these platforms supports cutting-edge research at the interface of molecular biology and engineering.
Yes. We offer full sequence customization based on your experimental design, including sense/antisense strand selection and loop region design. In addition, we provide a wide range of chemical modifications (e.g., 2'-O-methyl, phosphorothioate linkage, cholesterol conjugation, Cy3/Cy5 labeling) to enhance nuclease resistance, stability, delivery efficiency, or fluorescence detection.
Our technical team provides expert guidance during consultation. For example:
Yes. Our in vivo-grade miRNA reagents are synthesized under stringent conditions to ensure high stability, minimal immunogenicity, and endotoxin-free profiles. Agomirs, antagomirs, and cholesterol-conjugated miRNAs are particularly suited for animal delivery via systemic injection, local administration, or hydrodynamic delivery.
Yes. We can synthesize miRNA cocktails, dual-targeting constructs, and multi-binding site sponges to help study miRNA networks, synergistic regulation, or redundant gene suppression. This is particularly useful in cancer, stem cell, and immune regulation studies where miRNA crosstalk is critical.
Yes. We ship globally with lyophilized formulations that remain stable during transit. For temperature-sensitive products, we use validated cold chain logistics with real-time temperature monitoring and customs documentation to ensure smooth clearance and integrity upon arrival.
Global Supplier of High-Quality RNA / DNA Products & Services.
