Custom miRNA Agomir Synthesis

miRNA agomir is a specially labeled and chemically modified double-stranded small RNA. BOC Sciences provides miRNA agomir synthesis services to accelerate miRNA research progress.

What is miRNA Agomir

• Introduction to miRNA

miRNA, also known as microRNA, is an RNA molecule of 21-23 nt in length that widely exists in eukaryotes. miRNA inhibits mRNA transcription by binding to mRNA. Therefore, it plays an important role in regulating gene expression, cell cycle, and developmental events of organisms.

Fig. 1 miRNA biogenesis and functions (Alsharafi WA, 2015)

• Definition of miRNA Agomir

miRNA agomir is a specially labeled and chemically modified double-stranded small RNA (miRNA agonist) that regulates the biological functions of target genes by mimicking endogenous miRNA into the miRiSC complex.

• Basic Principle of miRNA Agomir

miRNA agomir is a synthetic miRNA molecule that mimics natural miRNA molecules, binding to mRNA of target genes and inhibiting their translation and expression. Compared to traditional siRNAs, miRNA agomir has a broader targeting and longer-lasting effect. miRNA agomir synthesis requires strict quality control and purification to ensure its safety and efficacy.

• Application Scenarios for miRNA Agomir

miRNA agomir has a wide range of application scenarios and can be used to treat a variety of diseases, such as cancer, cardiovascular diseases, diabetes, etc. As an example, miR-34a agomir can inhibit the proliferation and metastasis of tumor cells, thus enabling tumor treatment. In addition, miRNA agomir can be used in gene editing, stem cell therapy, and other fields.

Our miRNA Agomir Synthesis Service Content

1. Synthesize miRNA agomir - Synthesize the corresponding miRNA agomir according to the miRNA sequence information provided by customers.
2. Purify miRNA agomir - Purify the synthesized miRNA agomir by ion exchange chromatography, reverse phase high performance liquid chromatography and other techniques to improve the product purity.
3. Identify miRNA agomir - Use techniques such as mass spectrometry and nuclear magnetic resonance to identify the synthesized miRNA agomir to ensure the quality and purity of the product.
4. Packaging miRNA agomir - The purified miRNA agomir will be packaged according to customer requirements, and different sizes of packaging can be provided, such as milligram level, microgram level, etc.
5. Provide warranty service - Provide warranty service for miRNA agomir to ensure the quality and stability of the product.
6. Provide technical support - Provide the usage and technical support of miRNA agomir to ensure customers can use the product smoothly.

Advantages of BOC Sciences' miRNA Agomir Service

• Covers human, mouse, rat miRNA
• miRNA agomir possessed a higher affinity with cell membrane than common miRNA mimics and miRNA inhibitors
• Higher stability and inhibitory
• Enriched in target cells to achieve efficient specificity and stable interference
• Suppression lasts over an extended period(at least one week) and interference effect lasts up to 5-6 weeks

Frequently Asked Questions (FAQ)

What is miRNA Agomir and how does it work?

miRNA Agomir is a specially labeled, chemically modified double-stranded small RNA that mimics endogenous miRNAs. It binds to target mRNA molecules and regulates gene expression by inhibiting translation, making it a powerful tool for gene silencing and regulation.

What are the applications of miRNA Agomir?

miRNA Agomir is widely used in research fields such as gene regulation, gene editing, and stem cell therapy. It can also be applied to study gene functions, explore regulatory networks, and enhance the specificity and longevity of RNA interference experiments.

How does miRNA Agomir differ from traditional RNA interference methods like siRNA?

miRNA Agomir offers broader targeting and longer-lasting effects compared to traditional siRNA. It is more stable, has higher affinity for cell membranes, and can achieve more efficient gene silencing with sustained interference lasting up to 5-6 weeks.

How is miRNA Agomir synthesized and purified?

At BOC Sciences, miRNA Agomir is synthesized using advanced chemical methods and then purified through techniques like ion exchange chromatography and reverse-phase high-performance liquid chromatography (HPLC) to ensure high purity and activity.

What quality control measures does BOC Sciences implement for miRNA Agomir?

We use mass spectrometry and nuclear magnetic resonance (NMR) to identify and confirm the purity and structure of synthesized miRNA Agomir. This ensures the product's quality and suitability for downstream applications.