Oligo Fluorescent Labeling

BOC Sciences offers a complete oligonucleotide fluorescent labeling service. Our fluorescent labeling includes 6-FAM, HEX, and TET, among others. Our custom services are designed to meet your requirements through strict quality control and fast turnaround, providing scientists with the necessary tools to meet the most challenging applications. You can also send us your reactive dyes and we can label them for you quickly and efficiently.

Introduction

Fluorescent labeling is by far the most commonly used labeling for oligonucleotide synthesis, and it is possible to transduce analyte-bioreceptor binding into analytical signals by attaching one or more fluorophore groups or fluorescent compounds to oligonucleotide chains. Oligo fluorescent labeling is extensively used in gene sequencing, forensic and genetic analysis.

• Hexachloro-fluorescein (HEX)

HEX is the hexachloro derivative of carboxyfluorescein with a maximum absorbance of 535 nm and a maximum emission wavelength of 556 nm, which is red.

• 5'-Dichloro-dimethoxy-fluorescein dye (JOE)

JOE is a conventional fluorophore with a maximum excitation wavelength of 520 nm and a maximum emission wavelength of 548 nm.

• Tetrachloro fluorescein (TET)

TET is a highly stable dye that modifies the 5' end of oligonucleotides. TET has a maximum absorbance of 521 nm, a maximum emission wavelength of 536 nm, and an orange color.

• Rhodamine red X

Rhodamine red X is a sulfonated xanthene dye with a maximum absorbance of 570 nm and a maximum emission wavelength of 590 nm.

Synthetic Methods

• Direct oligonucleotide labeling during chemical synthesis

This method is based on the solid-phase synthesis, with nucleoside phosphoramidites as key building blocks, and is compatible with almost any natural or non-natural modifications as long as undesired side reactions are avoided during synthesis and reactive hydroxyl and amino groups are protected.

• Post-synthetic fluorescent oligo conjugation

The linkage of fluorescent dyes to oligonucleotides can be achieved by using reactive dyes containing functional groups such as thio, aldehyde, azide, amino and carboxyl groups as well as modified enzymes. After proper HPLC processing, the corresponding product can be obtained.

Our Oligo Fluorescent Labeling Services Specification

Advantages

• High-quality products
• competitive price
• Multiple labels to choose from
• Data analysis, detailed reporting including results and discussion
• All samples are carefully monitored for stability and characterized to ensure batch-to-batch consistency.

Application

• Nucleic acid sequencing
• Detection of pathogenic pathogens
• Genetic disease, diagnosis of tumor
• Gene expression analysis