With many years of experience in engineered nucleic acid production, BOC RNA has accumulated a wealth of phosphoramidite technology expertise in protection chemistry, modification, production scale-up, and innovative purification strategies. We provide high-quality phosphoramidites for RNA oligonucleotide synthesis. All standard (and customized) phosphoramidite production is strictly controlled by process and quality.
Our phosphoramidite products include RNA phosphoramidites, 2’-OMe-phosphoramidites, 2’-F-phosphoramidites, 2’-MOE-phosphoramidites, 3’-TBDMS-RNA phosphoramidites, 5-modified phosphoramidites, etc.
| Phosphoramidites | Features |
| RNA phosphoramidites |
|
| 2’-OMe-phosphoramidites |
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| 2’-F-phosphoramidites | |
| 2’-MOE-phosphoramidites | |
| 3’-TBDMS-RNA phosphoramidites | |
| 5-modified phosphoramidites |
Custom manufacturing services available to help develop or expand phosphoramidite compounds
Phosphoramidites are activated nucleotide derivatives used as building blocks for RNA oligonucleotide synthesis. They enable precise sequence assembly for research applications such as gene design, antisense, siRNA, and CRISPR-Cas9 gRNA construction.
2'-OMe and 2'-F modifications increase oligonucleotide stability and resistance to enzymatic degradation, improving sequence fidelity and performance in biochemical and molecular biology applications.
Each batch is monitored from raw material procurement to final release, with purity assessed using HPLC and ^31P NMR, ensuring minimal impurities and consistent performance across synthesis applications.
Custom services allow modification of sugar, base, or phosphate components, enabling researchers to develop tailored oligonucleotide sequences for advanced studies such as gene editing or novel RNA constructs.
BOC RNA provides a range of phosphoramidites, including standard RNA, 2'-OMe, 2'-F, 2'-MOE, 3'-TBDMS, and 5-modified phosphoramidites, supporting diverse applications from high-fidelity RNA synthesis to genome editing.
Chemical modifications can enhance hybridization specificity, increase thermal stability, and reduce secondary structure formation, improving efficiency in PCR, sequencing, and RNA-guided molecular experiments.
