Ferrocene Labeling of Oligonucleotides

What is Ferrocene Labeling Oligonucleotides?

Ferrocene Labeling Oligonucleotides is a labeling strategy that introduces a Ferrocene moiety into an oligonucleotide molecule. This labeling is achieved by synthetically attaching the Ferrocene moiety precisely to a specific position in the oligonucleotide. Ferrocene is a molecule with specific electrochemical properties, so the introduction of this moiety results in labeled nucleic acid molecules with unique electrochemical properties. This labeling strategy can be used to study the electrochemical behavior of DNA and RNA, including their redox properties under different conditions.Ferrocene Labeling Oligonucleotides can also be applied in electrochemical sensors and biosensors by monitoring the electrochemical signals of the Ferrocene to enable the detection of nucleic acid molecules. Such labeled nucleic acid molecules have potential applications in biomedical and analytical chemistry, providing powerful tools for studying biomolecular interactions and developing novel biosensors.

Figure 1. Ferrocene-labeled oligonucleotide chains end-tethered to gold electrode surfaces. (A, Agne.; et al, 2003)

Applications of Ferrocene Labeling Oligonucleotides

• Electrochemical Analysis: Ferrocene Labeling Oligonucleotides can be used to study the redox properties of DNA and RNA under electrochemical conditions. This aids in understanding the electrochemical behavior of nucleic acid molecules and provides a basis for the design of electrochemical sensors.
• Biosensor Development: Nucleic acid molecules can be used in biosensors by introducing Ferrocene labeling. These sensors can detect specific nucleic acid sequences and achieve highly sensitive and selective nucleic acid detection by monitoring the electrochemical signal of Ferrocene.
• Optimizing Oligonucleotide Drug Delivery: The introduction of Ferrocene Labeling Oligonucleotides can also be used in nucleic acid drug delivery systems. Such labeling can provide additional functionality, such as altering the hydrophilicity of the nucleic acid or the permeability of the cell membrane, thereby enhancing the efficiency of nucleic acid delivery within the cell.
• Gene Therapy Development: In the biomedical field, Ferrocene-labeled nucleic acid molecules can be used to study gene expression, gene regulation, and other biological processes that interact with nucleic acids.

BOC Sciences Offers Ferrocene Oligonucleotides

As an expert in nucleic acid synthesis, BOC Sciences mentions the introduction of Ferrocene labeling into oligonucleotide molecules as a custom modification service, which imparts special electrochemical properties to nucleic acids and assists in the field of electrochemistry and sensor development. The service processes we have used for optimization include sequence design, synthesis and labeling, purification and characterization, and even biological function evaluation.

BOC Sciences offers the following ferrocene labeling services.

• Terminal Ferrocene Labeling: Ferrocene labeling is attached to the end of an oligonucleotide, usually the 5' end or the 3' end. This type of labeling is commonly used to study the electrochemical properties of Ferrocene in oligonucleotide molecules.
• Internal Ferrocene Labeling: Ferrocene labeling is introduced at an internal position in the oligonucleotide sequence. This type of labeling may involve multiple Ferrocene labeling sites for specific research purposes such as electrochemical detection and analysis.
• Multiple Ferrocene Labeling: The introduction of multiple Ferrocene markers on an oligonucleotide molecule can increase the sensitivity and specificity of the electrochemical signal. This is useful for some highly sensitive electrochemical studies.
• Site-specific Ferrocene Labeling: Ferrocene labeling is introduced at specific base positions on oligonucleotides to study the interaction of Ferrocene with specific structures of DNA /RNA .
• Functional Ferrocene Labeling: Ferrocene labeling may be attached to specific functional groups to achieve specific properties or functions of the oligonucleotide molecule, such as for biosensors or drug delivery .
• Reversible Ferrocene Labeling: Some designs also include reversibly attached Ferrocene markers to enable modification or release of the oligonucleotide when needed.

Based on industry-leading oligonucleotide synthesis technology platforms and state-of-the-art equipment, more tailor-made services not mentioned are available at BOC Sciences. Please relax and contact us, we always look forward to partnering with you.

Reference

1. A, Agne.; et al. 3'-Ferrocene-Labeled Oligonucleotide Chains End-Tethered to Gold Electrode Surfaces: Novel Model Systems for Exploring Flexibility of Short DNA Using Cyclic Voltammetry.J. AM. CHEM. SOC.2003, 125: 1112-1113.

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Ferrocene Labeling of Oligonucleotides

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