Custom BNA Oligonucleotide Synthesis

As a leading biotechnology company, BOC Sciences provides bridging nucleic acid (BNA) oligonucleotide synthesis services to customers worldwide with its proprietary nucleic acid technology. The third-generation nucleic acid analog, BNA, has better affinity and stability, and can better meet the specific needs of customers.

BOC Sciences' BNA Oligos Synthesis Service

What is Bridged Nucleic Acid?

Bridging nucleic acid (BNA) is a novel nucleic acid analogue. The first generation BNA, LNA, is a bicyclic nucleotide analogue. LNA has good RNA-specific binding affinity. The second generation BNA, BNA^NC (2'-O,4'-amino ethylene bridging nucleic acid) is a six-membered bridging oligonucleotide. Oligonucleotides containing BNA^NC are more thermostable and water-soluble and lead to RNase H degradation of the target RNA. The new generation of BNA contains a five-membered or six-membered bridged structure with a fixed" C3'-endo sugar puckering. This bridge binds to the 2', 4' position of the ribose to obtain a 2', 4'-BNA monomer. The potential of the new generation BNA is the iteration of various BNAs. Such as, the recent synthesis of (S)-cEt-BNA, amino-BNA (AmNA), sulphonamide-BNA, guanidino-BNA and benzylidene acetal-type BNA. Compared with the previous generations of restricted nucleic acids (LNA or BNA^NC), the new generation of BNA exhibits a remarkably high affinity for its complementary strand. Their extraordinary levels of sensitivity and specificity for nucleic acid resistance make BNAs an excellent tool for the development of high-value detection systems and therapeutic products.

Fig. 1 The structure of BNA^coc and other 2', 4' BNA^NC nucleic acids. (Kim S, 2015)

Advantages of BNA Oligos

Incorporation of BNA into oligonucleotides allows the production of modified synthetic oligonucleotides with the following characteristics:

• Strong binding affinity to complementary single-stranded DNA or RNA
• Improve selectivity and specificity of hybridization
• Assess its double-strand formation ability for ssDNA or dsRNA
• Better resistance to nucleases and better flexibility
• Compared with common DNA or RNA oligonucleotides, the resulting oligonucleotides have good water solubility

Applications of BNA Oligos

• DNase and ribozyme
• Biosensor
• Antigen inhibition
• Gapmer antisense studies
• Inhibition of RNA function
• In vivo and in vitro delivery
• RNAi
• RT-PCR
• AS-PCR
• ISH

Frequently Asked Questions (FAQ)

What distinguishes BNA from earlier nucleic acid technologies like LNA?

BNA incorporates advanced bridged structures with optimized ring sizes that provide superior binding affinity, enhanced nuclease resistance, and improved specificity compared to first-generation LNA technology.

How does BNA modification improve oligonucleotide performance in research applications?

BNA modifications significantly increase thermal stability, enhance hybridization specificity, and improve resistance to enzymatic degradation, making them ideal for demanding applications requiring high precision.

What types of BNA modifications are available for custom synthesis?

Multiple BNA variants including 2',4'-BNA, BNANC, and novel bridged structures are available, each offering distinct advantages in affinity, specificity, and biochemical properties.

Can BNA be combined with other modifications in oligonucleotide design?

Yes, BNA can be effectively integrated with various backbone modifications, fluorescent labels, and functional groups to create multifunctional oligonucleotides for complex research applications.