As a common RNA methylation modification, m7G modification refers to the modification of adding a methyl group to the 7th N-position of RNA guanine (G). A variety of clinical data suggests that m7G modification enables to affect tRNA function and many important cellular processes in living organisms, and m7G has become a new biomarker with important regulatory functions. Therefore, in order to further understand the role of this kind of modification in various physiological processes, scientists have applied various sequencing technologies to perform the accurate identification of the modification site. BOC Sciences' professional epitranscriptomics & sequencing experts and bioinformatics teams provide in-depth and comprehensive tRNA m7G methylation sequencing and data analysis services.
Fig 1. Positions of the m7G modification in tRNA. (Tomikawa C, 2018)
BOC Sciences provides a variety of m7G RNA methylation sequencing services:
We provide MeRIP-Seq services by using pre-validated commercial antibodies and carefully optimized experimental procedures.
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We offer a TRAC-Seq service for the unbiased global mapping of m7G modification of tRNAs. Our methods combine the selection of small tRNA molecules, AlkB demethylation, and sodium borohydride reduction steps to achieve specific and efficient single-nucleotide resolution analysis of m7G sites in tRNAs.
Our Sequencing Workflow
Fig 2. The formation of m7G modification. (Tomikawa C, 2018)
BOC Sciences provides reliable m7G-quant-seq service, and our experts use chemometric information to detect abasic sites of m7G modifications on tRNA at single-base resolution. Through efficient chemical reduction and mild depurination, we can completely convert almost all m7G sites into RNA abasic sites.
Our Sequencing Workflow
Reference
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