Rhodamine Labeling of Oligonucleotides

Rhodamine dyes are a class of commonly used fluorescent dyes that can be used to label a variety of biomolecules, including oligonucleotides. BOC Sciences provides nucleic acid labeling services and can perform rhodamine labeling of oligonucleotides according to customer needs.

What is Rhodamine?

Rhodamine is a 3',6' diaminylated xanthene fluorescent dye. Compared to other fluorescent dyes (hydroxylated xanthene), the fluorescence of rhodamine (aminated xanthene) has better pH stability, and aminated dyes are more likely to be structurally diverse, with different structures yielding different wavelengths and colors of fluorescence, making rhodamine-type dyes available in a wider range of fluorescent colors. There are various structures of rhodamine, the commonly used ones are Rhodamine green, Rhodamine 6G, Tetramethylrhodamine (TMR), Rhodamine B, Lissamine rhodamine, X-rhodamine, Texas red, silicon rhodamine (SiR), etc.

Characteristics of Rhodamine Dyes

• Strong fluorescent signal

Rhodamine dyes produce bright and stable fluorescence, which contributes to highly sensitive detection and visualization.

• Wide wavelength range

Rhodamine dyes have a wide range of absorption and emission wavelengths in the visible spectral range, from red to near-infrared, allowing for multi-channel or multi-labeling experiments for multiple fluorescent labeling and detection.

• Chemical stability

Rhodamine dyes have high chemical stability and can maintain their fluorescence performance under different experimental conditions.

Services for Rhodamine-Labeled Oligonucleotides

BOC Sciences currently offers Rhodamine dye labeling types including the following.

• Rhodamine green labeled oligonucleotides
• Rhodamine 6G labeled oligonucleotides
• TMR labeled oligonucleotides
• Rhodamine B labeled oligonucleotides
• Lissamine rhodamine labeled oligonucleotide

• Optimization of Rhodamine labeled oligonucleotides

• Choosing the right reaction conditions

The conditions of the labeling reaction should be optimized for the specific reagents and sequences of oligonucleotides you are using. Different reagents and nucleic acid sequences may require different reaction temperatures, reaction times, buffer conditions, etc.

• Controlling the pH of the reaction

Labeling reactions with Rhodamine dyes are usually performed under alkaline conditions. Ensure that the pH of the buffer is appropriate to facilitate the reaction.

• Verification of labeling effect

After completing the labeling, validation of the labeled product is required. We typically use a fluorescence spectrometer to perform fluorescence measurements and compare them to unlabeled oligonucleotides. Make sure that the labeled oligonucleotide shows a clear fluorescence signal and that the fluorescence intensity is proportional to the amount of labeling.

• Steps for labeling oligonucleotides with rhodamine dye

Advantages of BOC Sciences' Rhodamine Labeling Services

• Rich Experience

We have rich experience and good reputation to ensure the quality and accuracy of labeling.

• Customized Capability

We can provide customized oligonucleotide labeling services according to your requirements, including the selection of appropriate Rhodamine dyes and labeling positions.

• Delivery Time

We can accurately keep track of delivery times when researching your project, thus helping you to better manage your time.

BOC Sciences is committed to providing high-quality Rhodamine-labeled oligonucleotide synthesis products. If you are interested in our services, feel free to contact us.

Online Inquiry

Rhodamine Labeling of Oligonucleotides

- mg g kg ton

We recommend you to leave us your work email address so that we can serve you better. Please leave your organization information here if you don't have a work email address.

SUBMIT