BOC Sciences focuses on advancing drug delivery development through bioconjugation. Relying on our state-of-the-art manufacturing facilities and comprehensive experience in conjugation technologies, BOC Sciences offers conjugation services to combine oligonucleotides with cell-penetrating peptides(CPPs). We have the capability and confidence to meet your needs for novel conjugates.
Cell-penetrating peptide (CPP), also known as protein transduction domain (PTD), is a short peptide that plays an important role in intercellular material transport. CPP is capable of entering cells and transporting various types of molecules, such as proteins, nucleic acids and nanoparticles across cell membranes.
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Figure 1. Glycosylated cell-penetrating peptides(GCPPs).( Gallego, I; et al, 2019)
The main role of CPP in CPP-oligonucleotide conjugation is to enhance the cellular uptake and intracellular delivery of oligonucleotides, thus improving their bioavailability and efficacy, ultimately leading to enhanced therapeutic or imaging effects.
| Service | CPP-Oligonucleotide Conjugation |
| Customer provides | Oligonucleotide sequence |
| Purification | QC analysis by MS, HPLC, or PAGE |
| Delivery | Typical delivery is lyophilized samples in individual vials that are fully labeled |
| Request a Quote | Inquiry |
1. CPP-Oligonucleotide Conjugation Synthesis: The preparation of CPP-oligonucleotide conjugates usually involves two main steps. The first is the synthesis or acquisition of CPP and oligonucleotides, followed by the selection of a suitable chemical coupling strategy to couple them together. BOC Sciences offers the following coupling strategies for the synthesis of CPP-Oligonucleotide Conjugation.
2. Conjugation Purification & Identification: BOC Sciences has optimized purification and evaluation methods to ensure the quality of the conjugates.
3. Detailed Analytical Reports: BOC Sciences will provide you with a detailed conjugate-based identification report at the end to ensure high quality delivery.
Figure 2. Service flow of CPP-oligonucleotide conjugation.
Ultra-high purity
BOC Sciences' unique separation, purification and characterisation solutions ensure high purity of conjugation products.
Efficient Delivery Times
BOC Sciences' large-scale synthesis capabilities are recognised by many customers, ensuring on-time or even early delivery.
Technical Support from Experienced Experts
With decades of experience in the synthesis of oligonucleotides, BOC Sciences' experts provide expert technical support and advice.
On-demand Customisation Available
Based on a proprietary oligonucleotide coupling customisation solution, BOC Sciences offers a flexible on-demand customisation service.
CPP-oligonucleotide conjugation involves linking cell-penetrating peptides (CPPs) with oligonucleotides to enhance the cellular uptake and intracellular delivery of oligonucleotides. This conjugation improves the bioavailability of the oligonucleotides and optimizes their therapeutic or imaging efficacy.
Cell-penetrating peptides (CPPs) are short peptides that can cross cell membranes efficiently. When conjugated with oligonucleotides, CPPs promote their entry into cells, enhancing their stability and effectiveness in intracellular environments, which is particularly useful for gene therapy and drug delivery.
CPPs are conjugated to oligonucleotides through covalent coupling, disulfide bond formation, or non-covalent complexation, depending on the application and desired outcome.
CPP-oligonucleotide conjugates are used in gene therapy, antimicrobial therapy, targeted delivery, and diagnostics by improving the efficiency and specificity of oligonucleotide delivery.
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