Solid Phase Oligonucleotide Synthesis

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With a state-of-the-art molecular biology platform, BOC Sciences' experienced oligonucleotide R&D and production team provides you with high-quality, customized, large-scale oligonucleotide synthesis services. We are equipped with state-of-the-art equipment and technology to synthesize DNA and RNA oligonucleotides in the mg to kg range.

Fig 1. Schematic of the solid-phase synthesis of PS oligonucleotides. (Yang et al., 2018)

What is Solid Phase Oligonucleotide Synthesis?

Oligonucleotides can be synthesized by solid-phase phosphoramidite chemistry, and the synthesis method is the solid-phase phosphoramidite method (referred to as solid-phase synthesis). The synthesis vehicle plays an important role in the solid phase synthesis reaction. Commonly used carriers include controllable microporous glass (CPG) and polystyrene (PS). CPG carriers have the advantages of good mechanical properties, insolubility, and controllable pore size. PS carriers have the advantage of high loading (350 μmol/g) and can be applied as a large-scale synthesis of oligonucleotide carriers. Solid-phase synthesis of oligonucleotides is an important technique to synthesize short nucleic acid sequences by adding nucleotide units one by one on a solid-phase support. With high purity, high yields and a wide range of applications, this synthesis method is important for nucleic acid-related work in research and applications.

Advantages of Solid Phase Synthesis of Oligonucleotide

Solid phase synthesis is carried out on a solid support between filters in a column that allows all reagents and solvents to pass freely. Solid phase synthesis offers many advantages over solution synthesi.

Large excesses of solution phase reagents are available to drive the reaction to rapid completion.
Impurities and excess reagents are washed away and purification is not required after each step.
The process lends itself to automation on a computer-controlled solid phase synthesizer.

Steps in Solid Phase Synthesis

Each cycle of solid phase synthesis consists of four main steps: deprotection, coupling, oxidation, and capping. Each cycle attaches a new nucleotide until the oligonucleotide chain is extended to the desired length. The oligonucleotide is then treated with ammonia to cut the oligonucleotide from the solid phase carrier and remove the protective groups, followed by a series of purification, desalting and lyophilization steps to obtain the final purified oligonucleotide.