Stable expression cell lines refer to the consistent and stable expression of a specific gene or interference with the expression of a specific gene in a cell. The plasmid DNA of the target gene of a stable cell line is integrated into the cell chromosome so that the cell consistently expresses the gene or consistently interferes with the target gene over a long period of time. BOC Sciences has expertise in stable cell line construction and we can perform RNAi stable cell line screening services as required by our clients.
Stable transfection is the integration of the transfected plasmid DNA into the chromosome of the host cell so that the host cell can express the target gene and protein for a long time. It is necessary to screen target cells based on transient transfection or use high-transfection-efficiency viruses, and select corresponding drugs according to the resistance markers contained in different gene carriers for cell passage, to obtain cell lines that can stably express the target gene.
Stable cell line screening methods: